- |||||||||| Review, Journal: Human Borrelia miyamotoi Infection in North America. (Pubmed Central) - Apr 28, 2023
Antibiotics are effective in clearing infection and are the same as those used for Lyme disease, including doxycycline, tetracycline, erythromycin, penicillin, and ceftriaxone. Preventive measures include avoiding areas where B. miyamotoi-infected ticks are found, landscape management, and personal protective strategies such as protective clothing, use of acaricides, and tick checks with rapid removal of embedded ticks.
- |||||||||| doxycycline / Generic mfg.
Journal: Caveats of Using Overexpression Approaches to Screen Cellular Host IFITM Proteins for Antiviral Activity. (Pubmed Central) - Apr 28, 2023
Previously, we used a doxycycline-inducible overexpression system, in conjunction with approaches to modulate the expression of endogenous protein, to demonstrate the antiviral activity of IFITM1, IFITM2, and IFITM3 against influenza A virus (IAV) but not parainfluenza virus-3 (PIV-3) in A549 cells...We propose that exceedingly high levels of overexpressed IFITMs may not accurately reflect the true function of endogenous protein, thus contributing to discrepancies when attributing the antiviral activity of individual IFITM proteins against different viruses. Our findings clearly highlight the caveats associated with overexpression approaches used to screen cellular host proteins for antiviral activity.
- |||||||||| Journal: Towards a Standardized Antimicrobial Susceptibility Testing Method for Mycoplasma hyorhinis. (Pubmed Central) - Apr 28, 2023
With this newly proposed method, we aim to provide an improved AST method for diagnostic laboratories and monitoring purposes with better comparability between times and countries. In addition, this new method will allow for an improvement of targeted treatments using antimicrobial agents and thereby reduce the options for resistance development.
- |||||||||| doxycycline / Generic mfg.
Journal: Role of Doxycycline as an Osteoarthritis Disease-Modifying Drug. (Pubmed Central) - Apr 28, 2023
Current evidence does not favor the regular use of doxycycline for the treatment of osteoarthritis as an individual treatment option or in combination with others. However, multicenter large cohort studies are warranted to determine the long-term benefits of doxycycline.
- |||||||||| doxycycline / Generic mfg., tetracycline / Generic mfg., sulphadiazine / Generic mfg.
Journal: Assessment of Heavy Metals Contamination and Antimicrobial Drugs Residue in Broiler Edible Tissues in Bangladesh. (Pubmed Central) - Apr 28, 2023
Irrespective of the source, broiler meat was found to contain antimicrobial drugs and heavy metals residues below the MRL, except for lead, suggesting that broiler meat is safe for human consumption. Therefore, raising public awareness regarding misconceptions about broiler meat consumption among consumers would be warranted.
- |||||||||| doxycycline / Generic mfg.
Journal: Doxycycline Attenuates Pig Intestinal Microbial Interactions and Changes Microbial Metabolic Pathways. (Pubmed Central) - Apr 28, 2023
Functional prediction showed that doxycycline significantly altered metabolic pathways related to the cell membrane. The results revealed that the use of doxycycline during pig breeding can affect bacterial abundance during the withdrawal period, and it may affect interactions among bacteria and change the intestinal metabolic pathways.
- |||||||||| doxycycline / Generic mfg.
Journal: Identification of Cutibacterium modestum in Spondylitis by Metagenomics Analysis. (Pubmed Central) - Apr 27, 2023
These results provide comprehensive genetic diversity, potential risks, and probiotic characteristics of Bacillus in the mariculture system in China, and provide basic support for green and healthy development of aquatic industry. This application of next-generation sequencing provides a new perspective in the clinical approach to spinal osteomyelitis and illustrates the potential of this technique in rapid etiological diagnosis.
- |||||||||| doxycycline / Generic mfg.
Journal: Green ecofriendly electrochemical sensing platform for the sensitive determination of doxycycline. (Pubmed Central) - Apr 27, 2023
This application of next-generation sequencing provides a new perspective in the clinical approach to spinal osteomyelitis and illustrates the potential of this technique in rapid etiological diagnosis. The electrochemical sensor, based on a screen-printed electrode modified with the hybrid material, was able to detect DOXY in the range of 5.1
- |||||||||| doxycycline / Generic mfg.
Genome-wide CRISPR-Cas9 screening to identify CDS2 as an essential gene in uveal melanoma. () - Apr 26, 2023 - Abstract #ASCO2023ASCO_6645;
Disruption of CDS2 results in reduced cellular fitness in uveal melanoma. Given the synthetic lethal relationship between CDS1 and CDS2, in the context of low CDS1 gene expression as observed in uveal melanomas and in other tumour types, CDS2 is a potential novel therapeutic target.
- |||||||||| Xpovio (selinexor) / Karyopharm, Menarini, FORUS Therap
Targeting Exportin 1 as treatment for cutaneous squamous cell carcinoma. () - Apr 26, 2023 - Abstract #ASCO2023ASCO_6625;
Additionally, cSCC cells had lower IC50 values for Selinexor, suggesting cSCC may be more sensitive to XPO1 inhibition compared to normal keratinocytes. Further, targeting XPO1 also increases apoptosis in these cells.
- |||||||||| doxycycline / Generic mfg.
Acute infectious purpura fulminans secondary to Rickettsial infection: A case series (Level 3 - Nicoll 2) - Apr 24, 2023 - Abstract #WCD2023WCD_2283;
All the cases were treated with doxycycline along with empirical antibiotics, anticoagulants, fresh frozen plasma, heparin, low molecular weight dextran and other supportive measures...The outcome was favorable with complete resolution of lesions in two children, residual gangrene and auto amputation of digits was noted in children who presented with peripheral gangrene. KEY MESSAGE: Purpura fulminans is a fatal condition, rickettsial infection causing PF is not uncommon, early diagnosis and timely initiation of treatment can reduce the morbidity and mortality
- |||||||||| doxycycline / Generic mfg.
Transactivator-Free, Doxycycline-Inducible CAR-T Cells (iTRUCKs) for Improved Antitumor Efficacy (Board No. 583) - Apr 21, 2023 - Abstract #ASGCT2023ASGCT_1383;
Dox addition to iTRUCK-1918, increased T stem cell memory/T central memory phenotypes, reduced exhaustion markers and polarize M2 (pro-tumor) to M1 (anti-tumor) macrophages in a co-culture system. These results showed that LOP LVs can generate iTRUCKs that can be controlled with Dox in vitro and in vivo, constituting a promising tool for improving CAR-T cell potency, while controlling side effects.
- |||||||||| doxycycline / Generic mfg.
LentiVEX Packaging Cell Line v2.0 Yields Comparable LVV Titres to the 4-plasmid Process (Board No. 794) - Apr 21, 2023 - Abstract #ASGCT2023ASGCT_1368;
It only requires transfection of the LV genome plasmid with doxycycline induction to achieve LVV yields of 2 x 108 TU/mL (with eGFP as Gene of Interest), comparable to the four-plasmid process with WXATUS0028...Scale-up vector production and process development with the packaging cell line are ongoing, with the Master Cell Bank (MCB) being generated for GMP-standard vector manufacturing. With a reduced plasmid cost and a simplified transfection process, the LentiVEX Packaging Cell Line v2.0 is a flexible platform to support both early-phase discovery studies with easy screening of multiple GOIs and large-scale LVV manufacture.
- |||||||||| doxycycline / Generic mfg.
Developing Cas13-ADAR-Mediated DUX4 mRNA Editing as a Prospective Therapy for FSHD (Board No. 672) - Apr 21, 2023 - Abstract #ASGCT2023ASGCT_1313;
Our strategy involved developing complex stable cell lines expressing doxycycline-inducible Cas13/ADAR; a transcriptionally active but non-toxic DUX4 ORF; and NanoLuciferase reporter gene driven by a DUX4-induced promoter...Our results may provide another treatment option for FSHD, and has implications for using this system to edit other transcripts. We will discuss prospects for uncovering better "rules" for designing effective RNA-editing gRNAs.
- |||||||||| chloroquine phosphate / Generic mfg., doxycycline / Generic mfg., sorafenib / Generic mfg.
Inhibition of c-MYC in Hepatocellular Carcinoma via AAV8 Mediated Gene Transfer (Board No. 600) - Apr 21, 2023 - Abstract #ASGCT2023ASGCT_1132;
Expression of Omomyc in HCC cell lines in vitro by transient transfection and doxycycline induced expression induces an 8-20 fold inhibition of proliferation depending on cell type, including G2 cell cycle arrest (about 50%) in the high c-MYC expressing cell line Hep3B...Our preliminary data suggests that this could be due to induction of autophagic cell death, with mitigation of the effect seen when treated with Bafilomycin A1 or Chloroquine...We have also demonstrated a potential synergistic effect of combining autophagy inducing compounds like sorafenib and mTOR inhibitors being able to increase transduction efficiency by approximately 50-60% in our HCC cells lines. Taken together, our data suggest that our novel AAV8 delivery of Omomyc has the ability to inhibit cellular proliferation, induce autophagy, and induce cell death in HCC which may prove to be a novel therapeutic approach for the treatment of HCC, especially when combined with conventional therapeutics.
- |||||||||| Journal: Comprehensive profiling of serotypes, antimicrobial resistance and virulence of Salmonella isolates from food animals in China, 2015-2021. (Pubmed Central) - Apr 21, 2023
Antibiotic susceptibility testing showing that the majority of the Salmonella isolates were resistant to neomycin (99.7%), tetracycline (98.1%), ampicillin (97.4%), sulfadiazine/trimethoprim (97.1%), nalidixic acid (89.1%), doxycycline (83.1%), ceftria xone (70.3%), spectinomycin (67.7%), florfenicol (60.0%), cefotaxime (52.0%) and lomefloxacin (59.8%)...However, the rate of resistance to fosfomycin were observed from higher to lower by S. Derby, S. Enteritidis, and S. Typhimurium...12 out of 17 virulence genes selected in this study were commonly presented in the chromosomes of tested isolate, with a detection rate of over 80%, including misL, spiA, stn, pagC, iroN, fim, msgA, sopB, prgH, sitC, ttrC, spaN. This study provided a systematical updating on surveillance on prevalence of Salmonella from food animals in China, shedding the light on continued vigilance for Salmonella in food animals.
- |||||||||| ceftriaxone / Generic mfg., doxycycline / Generic mfg.
AN UNEXPECTED CAUSE OF RIGHT UPPER QUADRANT ABDOMINAL PAIN IN A TEENAGER (E-Poster Area) - Apr 21, 2023 - Abstract #ESPID2023ESPID_653;
Learning Points/ Early detection and treatment of pelvic inflammatory disease is essential to prevent complications such as infertility. The nonspecific presentation of Fitz-Hugh-Curtis syndrome as a complication of pelvic inflammatory disease makes it a challenging diagnosis, however it should be considered in the differential diagnosis of right upper quadrant pain in the sexually active adolescent.
- |||||||||| doxycycline / Generic mfg.
SENLAT (SCALP ESCHAR AND NECK LYMPHADENOPATHY AFTER TICK BITE) CAUSED BY RICKETTSIA SLOVACA AND RICKETTSIA RAOULTII AFTER A TICK BITE: PAEDIATRIC CASE SERIES, PORTUGAL (E-Poster Area) - Apr 21, 2023 - Abstract #ESPID2023ESPID_642;
slovaca patient had eschar,fever,rash and adenopathies,while R. raoultii patients had fewer symptoms,1 only presenting scalp eschar with erythema.2/3 symptomatic patients underwent laboratory evaluation,unremarkable, and were treated with doxycycline with resolution of symptoms and without sequelae.In the 3 asymptomatic patients,2 ticks were infected with R. massiliae and 1 with R. monacensis...Molecular characterization of Rickettsia spp. and associated ticks removed from asymptomatic or symptomatic patients can contribute to a better understanding of rickettsial diseases.
- |||||||||| PEDIATRIC TUBULAR LYMPHANGITIS CAUSED BY RICKETTSIA SIBIRICA MONGOLITIMONAE (E-Poster Area) - Apr 21, 2023 - Abstract #ESPID2023ESPID_641;
He began treatment with oral azithromycin and intravenous amoxicillin-clavulanate, with a favorable evolution...Learning Points/ When LAR is suspected, empirical treatment with doxycycline should be started...In Spain, the human transmission of other life-threatening zoonotic agents by Hyalomma ticks, such as Crimean-Congo virus has already been reported. These potential risks emphasize the importance of the development of new prevention strategies and the evaluation of this threat to public health.
- |||||||||| doxycycline / Generic mfg.
Preclinical, Journal: Acute generation of reactive oxygen species that induced by doxycycline pretreatment results in rapid cell death in polyphyllin G-treated osteosarcoma cell lines. (Pubmed Central) - Apr 18, 2023
Co-administration of the ROS inhibitor NAC reversed the suppressed cell viability and colony-forming ability, and abolished the increased level of ?-H2A.X in the cells with the combined treatment, indicating that the enhanced ROS was involved in the anti-proliferative effect of the combined treatment. Overall, the results demonstrated that doxycycline may function as chemosensitizers by inducing an acute and lethal ROS production to enhance cytotoxic of polyphyllin G in osteosarcoma cell lines, and the combined use of drugs may provide an alternative thinking for the development of new therapeutic agents.
- |||||||||| lithium carbonate ER / Generic mfg.
Journal: An update on potential pharmacotherapies for cognitive impairment in bipolar disorder. (Pubmed Central) - Apr 17, 2023
Traditional treatments, such as lithium, anticonvulsants (lamotrigine), antipsychotics (aripiprazole, asenapine, cariprazine, lurasidone, and olanzapine), antidepressants (vortioxetine, fluoxetine, and tianeptine) and psychostimulants (modafinil), and emerging interventions, such as acetylcholinesterase inhibitors (galantamine and donepezil), dopamine agonists (pramipexole), erythropoietin, glucocorticoid receptor antagonists (mifepristone), immune modulators (infliximab, minocycline and doxycycline), ketamine, metabolic agents (insulin, metformin, and liraglutide), probiotic supplements, and Withania somnifera are discussed...In the past, methodological pitfalls in BD cognition trials have also been a critical limiting factor. Expanding on the existing literature and identifying novel pharmacological and non-pharmacological treatments for cognitive impairment in BD should be a priority.
- |||||||||| doxycycline / Generic mfg.
Journal, IO biomarker: Nanodrug Inducing Autophagy Inhibition and Mitochondria Dysfunction for Potentiating Tumor Photo-Immunotherapy. (Pubmed Central) - Apr 15, 2023
Herein, a liposome nanodrug co-encapsulating doxycycline hydrochloride (Doxy) and chlorin e6 (Ce6) to simultaneously induce autophagy inhibition and mitochondria dysfunction for potentiating tumor photo-immunotherapy is developed...The nanodrugs elicit remarkable antitumor therapy by combining Ce6-mediated PDT and Doxy-induced autophagy inhibition and mitochondria dysfunction. The developed nanodrugs represent a highly efficient strategy for improving cancer immunotherapy.
- |||||||||| doxycycline / Generic mfg.
Knockdown of a novel target gene enhances liver regeneration and attenuates chronic liver damage related liver fibrosis (Poster Area) - Apr 12, 2023 - Abstract #EASLILC2023EASL_ILC_651;
A genome-wide mouse shRNA library (shERWOOD-UltramiR) was cloned into transposon-based doxycycline (Dox)-inducible vector, divided into 32 pools and delivered into C57BL/6 mice liver using hydrodynamic-tail vein (HDTV) injections... Knockdown of a novel gene about which very little is known except that is a protein coding gene and enables RNA binding activity accelerates liver repopulation, regeneration and attenuates chronic liver damage related fibrosis.
- |||||||||| doxycycline / Generic mfg.
The unexpected effect of the protease CPAF from Chlamydia trachomatis is to increase the global synthesis of the host proteins (P445) (Exhibit Hall; Poster Board No. P445) - Apr 11, 2023 - Abstract #IMMUNOLOGY2023IMMUNOLOGY_1475;
T-REx-293 cells stably transfected with CPAF were induced with doxycycline and its effects on several known substrates were assayed by Western blot...More than 3000 MHC-I-restricted peptides were identified showing that the peptidome is altered when CPAF is induced, increasing the diversity and altering the physicochemical properties of the peptides.As conclusion, CPAF deeply alters the host proteome contributing to alter the peptide supply of the antigen processing pathway which affect the composition of the MHC-I-associated immunopeptidome. These data suggest that the generation of
- |||||||||| doxycycline / Generic mfg.
Beta cell expression of ST8Sia6 protects from autoimmune diabetes in the murine NOD model (P761) (Exhibit Hall; Poster Board No. P761) - Apr 11, 2023 - Abstract #IMMUNOLOGY2023IMMUNOLOGY_1316;
Flow cytometric analysis of islet infiltrating immune cells from euglycemic mice at 14 weeks of age demonstrated reductions in short-lived effector CD8 T cells and increased thymically derived Tregs in ?ST mice compared to littermate controls. These results highlight the therapeutic potential of sialyltransferase mediated immune regulation and present a potential path toward improved curative approaches for T1D.
- |||||||||| doxycycline / Generic mfg.
Investigating HIV-1 TAT protein role in Atherogenesis (P552) (Exhibit Hall; Poster Board No. P552) - Apr 11, 2023 - Abstract #IMMUNOLOGY2023IMMUNOLOGY_964;
To understand the contributions of TAT protein in HIV-associated atherogenesis, a doxycycline-inducible astrocyte-specific HIV-1 TAT transgenic mouse was used...Our preliminary data suggest that TAT is involved in the regulation of peripheral blood neutrophil numbers and therefore can serve as a regulator of inflammation in HIV-associated atherosclerosis. Future analysis will provide insight into potential mechanisms of HIV TAT-dependent involvement in atherosclerosis development and progression.
- |||||||||| doxycycline / Generic mfg.
Does intravenous vaccination with self-killing BCG lead to development of an immune response that protects against (Exhibit Hall; Poster Board No. P315) - Apr 11, 2023 - Abstract #IMMUNOLOGY2023IMMUNOLOGY_88;
ksBCG uses two transcriptionally repressible lysins; the inducer doxycycline is required for repression, with the absence of inducer leading to BCG death...Bronchoalveolar lavages were routinely taken throughout the study, and alongside tissue samples, analyzed using spectral flow cytometry. The presence of multiple cell types (CD4, CD8, and gd T cells, B cells, NK cells), cytokines (IFNg, IL-2, IL-17, TNF) and cytotoxic molecules (granzymes B and K, perforin, granulysin), as well as M. tuberculosis bacterial burden will be assessed to determine the level of protection provided by IV ksBCG.
- |||||||||| terbinafine HCL / Generic mfg.
A Rare Side Effect of Terbinafine () - Apr 6, 2023 - Abstract #AFMRSER2023AFMR_SER_14;
Rhabdomyolysis with ensuing acute kidney injury is not a well-known adverse effect of terbinafine. The purpose of this case report is to ensure providers are aware of this potential effect and monitor creatinine levels in patients taking this medication.
- |||||||||| doxycycline / Generic mfg.
Antigen Binding Domain Influences CD47-CAR T Cell Function in Both Constitutively Active and Inducible Systems (Board No. 614) - Apr 6, 2023 - Abstract #ASGCT2023ASGCT_595;
Despite limited expansion, B6H12-CAR T cells exert antitumor activity in vitro and, consistent with CV1-CAR T cells, lack antitumor activity in vivo likely due to macrophage mediated elimination.Given that multiple constitutively active CD47-CAR T cell products downregulate CD47 and fail to function in vivo, we generated CD47-CARs under control of a doxycycline inducible (Tet-On) promoter that constitutively express ZsGreen (ZsG) as a detection marker (indB6H12- and indCV1-CAR)...Overall, these results demonstrate the CAR binding domain influences CD47-CAR T cell functional characteristics in both constitutive and inducible systems.While studies are ongoing, we demonstrate that 1) similar to CV1-CAR T cells, B6H12-CAR T cells downregulate CD47 surface expression and lack antitumor activity in vivo and 2) CD47-CAR binding domain determines CAR surface expression upon activation in an inducible system. We speculate that CAR affinity or binding site on CD47 is responsible for differing CD47-CAR T cell functional phenotypes and studies are underway to elucidate mechanisms of these findings.
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