- |||||||||| doxycycline / generics
Journal: Leaky Expression of the TET-On System Hinders Control of Endogenous miRNA Abundance. (Pubmed Central) - Jun 17, 2019
...The authors show a significant reduction in cell proliferation and culture viability when maintained in media supplemented with the TET-On induction agent Doxycycline at concentrations commonly reported...We suggest a solution involving isolation of highly inducible clones and use a single cell analysis platform to demonstrate the heterogeneity of basal expression and inducibility. Finally, we describe numerous strategies to minimize leaky transgene expression and alterations to current miRNA sponge design.
- |||||||||| rifampicin / generics, doxycycline / generics
Journal: Brucella - Virulence Factors, Pathogenesis and Treatment. (Pubmed Central) - Jun 17, 2019
The treatment of brucellosis is based on two-drug therapy, the most common combinations of antibiotics are: doxycycline with rifampicin or fluoroquinolones with rifampicin. Currently, also other methods are used to disrupt Brucella intracellular replication (tauroursodeoxycholic acid or ginseng saponin fraction A).
- |||||||||| doxycycline / generics
Journal: An improved Tet-on system in microRNA overexpression and CRISPR/Cas9-mediated gene editing. (Pubmed Central) - Jun 16, 2019
Finally, the system was applied in CRISPR/Cas9-mediated knockout of nuclear factor of activated T cells-5 (NFAT5), a protective transcription factor in cellular osmoregulation. This study established an improved Tet-on system for powerful and stringent gene regulation in functional genetic studies.
- |||||||||| doxycycline / generics, gentamicin sulfate / generics, amoxicillin / generics
Journal: Antibiotic resistance of bacteria isolated from heavy metal-polluted soils with different land uses. (Pubmed Central) - Jun 15, 2019
The MIC profiles can be used to contribute towards a determination of an ECOFF for this species for these agents, which will aid in the setting and refining of clinical breakpoints for the most important antimicrobials used to treat melioidosis. The higher percentage of isolates with multiple antibiotic resistance in the agricultural soils that in the mining waste soils may be related to (1) the level of soil heavy metals, (2) the population and diversity of soil bacteria, (3) the application of manures, and (4) other factors affecting gene transfer between bacteria.
- |||||||||| doxycycline / generics
Retrospective data, Journal: Murine Typhus in South Texas Children: An 18-Year Review. (Pubmed Central) - Jun 15, 2019
Children who were treated earlier with doxycycline had a shorter hospitalization than were those who began therapy later. Recognition of murine typhus is important to prevent delay in treatment and development of complications.
- |||||||||| doxycycline / generics
Journal: Marine Develops Chronic Right Knee Granuloma After Initial Injury at The Basic School. (Pubmed Central) - Jun 15, 2019
Initial M. marinum infections can be mistaken as methicillin resistant Staph aureus infections in the active duty population. If the infection recurs or does not resolve with methicillin resistant Staph aureus-targeted antibiotics, consider cultures specific to acid-fast bacilli and keep in the differential until long term culture results are obtained.
- |||||||||| doxycycline / generics
Journal: Medical knowledge related to Rocky Mountain spotted fever in Sonora, Mexico. (Pubmed Central) - Jun 15, 2019
Rocky Mountain spotted fever (RMSF) is a tick-borne disease with a high case-fatality rate unless diagnosed promptly and treated timely with doxycycline...Inadequate medical knowledge may adversely affect how patients infected with Rickettsia rickettsii are diagnosed and treated. Educational programs that improve the risk perception and medical knowledge about RMSF should be targeted at physicians most likely to have initial contact with diseased patients.
- |||||||||| doxycycline / generics
Preclinical, Journal: Using TARGATT™ Technology to Generate Site-Specific Transgenic Mice. (Pubmed Central) - Jun 15, 2019
Using this approach, site-specific transgenic mice can be generated as fast as in 3 months. The technique presented here greatly facilitates murine transgenesis and precise structure/function dissection of mammalian gene function and regulation in vivo.
- |||||||||| Flagyl (metronidazole) / SLA
Review, Journal: Antibiotic Penetration into Bone and Joints: An Updated Review. (Pubmed Central) - Jun 15, 2019
Of note, studies on joint space penetration were fewer than studies on bone tissue penetration. Although clinical studies in osteomyelitis and septic arthritis are not available for all of the evaluated antibiotics, these pharmacokinetic results indicate that agents with good penetration profiles would have a potential utilization in such infections.
- |||||||||| doxycycline / Generic mfg., azithromycin / Generic mfg.
Trial completion date, Trial primary completion date: Trial of Azithromycin vs. Doxycycline for the Treatment of Rectal Chlamydia in MSM (clinicaltrials.gov) - Jun 14, 2019
P4, N=246, Recruiting,
SMA is common amongst Malaysian students and, despite understanding why SMA is unwise, even medical students self-medicate. Trial completion date: Oct 2019 --> Oct 2020 | Trial primary completion date: Jun 2019 --> May 2020
- |||||||||| doxycycline / generics
Clinical, Journal: Conditional Overexpression of rtn4al in Muscle of Adult Zebrafish Displays Defects Similar to Human Amyotrophic Lateral Sclerosis. (Pubmed Central) - Jun 9, 2019
After doxycycline induction, adult zebrafish displayed denervation at neuromuscular junction during the first week, then muscle disintegration and split myofibers during the third week, and, finally, significant weight loss in the sixth week. These results suggest that this zebrafish transgenic line, representing the inducible overexpression of Rtn4a1 in muscle, may provide an alternative animal model with which to study ALS because it exhibits ALS-like phenotype.
- |||||||||| Noxafil (posaconazole) / Merck (MSD)
Journal: Treatment of Aspergillus fumigatus infection with posaconazole delayed-release tablets. (Pubmed Central) - Jun 7, 2019
The results of this study highlight the importance of rational antimicrobial use and of further research on pharmacokinetics of antimicrobials and their degraded products in different environmental compartments, to efficiently control the spread of residues and/or resistance genes from manure to these matrices. A 13-year old patient with CF was successfully treated for an A. fumigatus infection with posaconazole delayed-release tablets.
- |||||||||| doxycycline / generics
Preclinical, Journal: Doxycycline improves cage activity, but not exercised, supraspinatus tendon and muscle in a rat model. (Pubmed Central) - Jun 7, 2019
Combined with exercise, doxycycline decreased average muscle fiber cross-sectional area. Results of this study suggest that administration of doxycycline at pharmaceutical doses induces beneficial supraspinatus tendon adaptations without negatively affecting the muscle in cage activity animals, supporting the use of doxycycline to combat degenerative processes associated with underuse; however, when combined with exercise, doxycycline does not consistently produce the same beneficial adaptations in rat supraspinatus tendons and reduces muscle fiber cross-sectional area, suggesting that doxycycline is not advantageous when combined with activity.
- |||||||||| doxycycline / generics
Journal: Generation of H11-albumin-rtTA Transgenic Mice: A Tool for Inducible Gene Expression in the Liver. (Pubmed Central) - Jun 6, 2019
...Expression of the H2BGFP fusion protein was observed exclusively upon doxycycline (Dox) induction in the liver of H11-albumin-rtTA/TetO-H2BGFP double transgenic mice...Finally, to characterize the expression-inducing effects of Dox in H11-albumin-rtTA/TetO-H2BGFP mice in detail, we examined GFP expression in embryos at different developmental stages and found that newly conceived H11-albumin-rtTA/TetO-H2BGFP embryos of Dox-treated pregnant female mice were expressing reporter GFP by E16.5. Our study demonstrates that these new H11-albumin-rtTA transgenic mice are a powerful and efficient tool for the temporally and spatially conditional manipulation of gene expression in the liver, and illustrates how genetic crosses with these new mice enable the generation of complex multi-locus transgenic animals for mechanistic studies.
- |||||||||| doxycycline / generics
Review, Journal: Update on Feline Hemoplasmosis. (Pubmed Central) - Jun 6, 2019
Although cats with hemoplasmosis respond rapidly to antibiosis and supportive care, initial monotherapy treatment rarely results in clearance of infection. A protocol now exists for the clearance of the most pathogenic feline hemoplasma M haemofelis."
- |||||||||| doxycycline / generics
Preclinical, Journal: Mouse embryo Hox gene enhancers assayed in cell culture: Hoxb4, b8 and a7 are activated by Cdx1 protein. (Pubmed Central) - May 31, 2019
...Cultures to be compared receive identical DNAs and differ only in whether or not they also receive inducer (doxycycline)...The magnitude of transfected chick Hoxa7 activation by Cdx1 is increased by multiple copies of its enhancer, but for maximum effect these must contain intact Cdx binding motifs. Cdx1 protein was found not to activate Hoxb4, b8 or a7 enhancers in P19 mouse pluripotential cells.
- |||||||||| doxycycline / generics
Journal: Yellow nail syndrome resulting from cardiac mitral valve replacement. (Pubmed Central) - May 30, 2019
Two prior reports involved with coronary artery bypass graft whereas we performed mitral valve replacement. In angiogram thoracic duct was not identified so that it seems post cardiac surgery Yellow nail the syndrome has iatrogenic origin due to the thoracic duct or its tributaries injury and requires meticulous assessment and management.
- |||||||||| doxycycline / generics
Journal: Molecular determinants of Burkholderia pseudomallei BpeEF-OprC efflux pump expression. (Pubmed Central) - May 29, 2019
...Acquired multidrug resistance, including resistance to doxycycline and co-trimoxazole used for melioidosis eradication phase therapy, is mainly attributed to constitutive expression of the BpeEF-OprC efflux pump...The binding site contains the conserved T-N11-A LTTR box motif involved in binding of LysR proteins, which in concert with two other possible LTTR boxes may mediate BpeT and BpeS regulation of BpeEF-OprC expression. These studies form the basis for further investigation of BpeEF-OprC expression and regulation at the molecular level by yet unknown external stimuli.
- |||||||||| doxycycline / generics
Journal: Reprogramming factors induce proliferation and inhibit apoptosis of melanoma cells by changing the expression of particular genes. (Pubmed Central) - May 29, 2019
...The results identified that expression of the four reprogramming factors was highly induced by doxycycline treatment in the stable melanoma cell clone that was transfected with a plasmid expressing these factors, driven by the Tet‑On element...Induced expression of these factors also decreased the apoptosis, as well as upregulated B‑cell lymphoma 2 (BCL‑2) and downregulated BCL‑2‑associated X (BAX) mRNA expression levels. Taken together, the results suggested that upregulated JAK2 and Cyclin‑B1 may be responsible for the enhanced proliferation of melanoma cells, and that BCL‑2 upregulation and BAX downregulation may account for the suppressed apoptosis of these cells.
- |||||||||| doxycycline / generics
Journal: Partial Rescue of Ocular Pigment Cells and Structure by Inducible Ectopic Expression of Mitf-M in MITF-Deficient Mice. (Pubmed Central) - May 29, 2019
...A new mouse line was created with doxycycline-inducible ectopic Mitf-M expression on an Mitf-deficient Mitfmi-vga9 background (DMV mouse)...Ectopic expression of Mitf-M during development of Mitf-deficient mice is capable of partially rescuing ocular and retinal structures and uveal melanocytes. These findings provide novel information about the roles of Mitf isoforms in the development of mouse eyes.
- |||||||||| doxycycline / generics
Journal: Elaborations on Corallopyronin A as a Novel Treatment Strategy Against Genital Chlamydial Infections. (Pubmed Central) - May 28, 2019
...Treatment failures using the standard regimens of doxycycline and azithromycin have been observed...Importantly, in an ex vivo human fallopian tube model, the growth of C. trachomatis was significantly inhibited by CorA at concentrations of 1-2 μg/mL under normoxic and hypoxic conditions. The overall high efficacies of CorA against C. trachomatis in cell culture and an ex vivo human fallopian tube model under physiological oxygen concentrations qualifies this drug as a candidate that should be further investigated.
- |||||||||| doxycycline / generics
Review, Journal: The Neuroprotective Effect of Doxycycline on Neurodegenerative Diseases. (Pubmed Central) - May 26, 2019
In this regard, a commonly used antibiotic, doxycycline (Doxy), has been shown to reduce the progression and severity of disease in different experimental models of neurodegeneration by counteracting these common features. This review will focus on the effects reported for Doxy regarding its neuroprotective properties, the "off-target" effects, thereby supporting its evaluation as a new therapeutic approach for diseases associated with a neurodegeneration.
- |||||||||| doxycycline / generics
Clinical, Journal: Doxycycline-induced photosensitivity in patients treated for erythema migrans. (Pubmed Central) - May 24, 2019
P=N/A
This review will focus on the effects reported for Doxy regarding its neuroprotective properties, the "off-target" effects, thereby supporting its evaluation as a new therapeutic approach for diseases associated with a neurodegeneration. Photosensitivity reactions in adult patients with EM treated with doxycycline 100 mg twice daily for 10-15 days occurred rarely, were not severe, and had no long-term sequelae.
- |||||||||| doxycycline / Generic mfg., Pierre Fabre
Clinical, Journal: Early Identification of Primary Hypothyroidism in Neonates Exposed to Intra-Lymphatic Iodinated Contrast: A Case Series. (Pubmed Central) - May 24, 2019
Patient 4 had a lymphatic malformation and underwent sclerotherapy with doxycycline with intralymphatic iodine exposure on DOL 4 and was found to have a TSH of 16.7 µU/mL (reference range, 1.7 to 9.1) 3 days later with spot urine iodine of 228,712 µg/L (normal, <200)...Intra-lymphatic iodine should be considered a major risk factor in the development of iatrogenic primary hypothyroidism, particularly in premature neonates soon after exposure. Close monitoring of TFTs is imperative to avoid potential long-term adverse outcomes in this population.
|
