- |||||||||| Journal: SARS-CoV-2 NSP13 Inhibits Type I IFN Production by Degradation of TBK1 via p62-Dependent Selective Autophagy. (Pubmed Central) - Jan 27, 2022
Finally, TBK1 and p62 degradation and p62 aggregation were observed during SARS-CoV-2 infection in HeLa-ACE2 and Calu3 cells. Overall, our study shows that NSP13 inhibits type I IFN production by recruiting TBK1 to p62 for autophagic degradation, enabling it to evade the host innate immune response, which provides new insights into the transmission and pathogenesis of SARS-CoV-2 infection.
- |||||||||| Journal, Cancer stem cells: Targeting cancer stem cells in medulloblastoma by inhibiting AMBRA1 dual function in autophagy and STAT3 signalling. (Pubmed Central) - Jan 26, 2022
Importantly, pharmacological inhibition of autophagy profoundly affects both stem and invasion potential of MB stem cells, and a combined anti-autophagy and anti-STAT3 approach impacts the MB outcome. Taken together, our data support the c-MYC/AMBRA1/STAT3 axis as a strong oncogenic signalling pathway with significance for both patient stratification strategies and targeted treatments of MB.
- |||||||||| Journal: SKP-SC-EVs Mitigate Denervated Muscle Atrophy by Inhibiting Oxidative Stress and Inflammation and Improving Microcirculation. (Pubmed Central) - Jan 26, 2022
To conclude, SKP-SC-EVs may alleviate the decrease of target muscle blood perfusion and passivate the activities of ubiquitin-proteasome and autophagy-lysosome systems by inhibiting oxidative stress and inflammatory response, then reduce skeletal muscle atrophy caused by denervation. This study not only enriches the molecular regulation mechanism of denervated muscle atrophy, but also provides a scientific basis for SKP-SC-EVs as a protective drug to prevent and treat muscle atrophy.
- |||||||||| Journal, Tumor Mutational Burden: High Level of Aristolochic Acid Detected With a Unique Genomic Landscape Predicts Early UTUC Onset After Renal Transplantation in Taiwan. (Pubmed Central) - Jan 26, 2022
Especially, mammalian target of rapamycin (mTOR) activation predominated in dA-AL-I-detected samples compared with those without dA-AL-I detection and might be associated with UTUC initiation through cell proliferation and suppression of UTUC progression via autophagy inhibition. Accordingly, dA-AL-I detection can provide more direct evidence to AA exposure and serve as a more specific predictive and prognostic biomarker for patients with de novo onset of UTUC after kidney transplantation.
- |||||||||| erastin - Whitehead Institute for Biomedical Research, Dana / Farber Cancer Institute, Columbia University, Prolexys
Journal: Iron metabolism regulation of epithelial-mesenchymal transition in idiopathic pulmonary fibrosis. (Pubmed Central) - Jan 26, 2022 To further ascertain the role of autophagy in erastin-induced EMT, chloroquine, which is an autophagy inhibitor, was employed, and was found to effectively reduce EMT in this process. These results support the role of the enhanced accumulation of iron as a mechanism for increasing the vulnerability of lung epithelial cells to iron-driven oxidant injury that triggers further autophagy during EMT.
- |||||||||| chloroquine phosphate / Generic mfg.
Journal, Combination therapy: Menin-mediated repression of glycolysis in combination with autophagy protects colon cancer against small molecule EGFR inhibitors. (Pubmed Central) - Jan 21, 2022 Inhibition of autophagy with chloroquine further sensitizes colorectal cancers to treatment with the combination of an EGFRi and menin inhibitor...Additionally, these findings illustrate the importance of autophagy as a protective mechanism against EGFRis, especially in the presence of menin inhibition. Ultimately this data opens the possibility of using menin-mediated regulation of glycolysis to potentially improve treatment modalities for CRC.
- |||||||||| cisplatin / Generic mfg.
NUMB PROMOTES AUTOPHAGOSOME MATURATION IN AKI THROUGH DDB1-CUL4 COMPLEX (Poster Area (Virtual)) - Jan 21, 2022 - Abstract #ISNWCN2022ISN_WCN_626; Numb plays a protective role in cisplatin induced AKI, through promoting the maturation of autophagosomes in tubule cells. Numb-DDB1-PI3KC3 may be a potential therapeutic target for AKI.
- |||||||||| cisplatin / Generic mfg.
MECP2 PROMOTES CISPLATIN-INDUCED ACUTE KIDNEY INJURY THROUGH EPIGENETIC REGULATION OF IRF8 (Poster Area (Virtual)) - Jan 21, 2022 - Abstract #ISNWCN2022ISN_WCN_608; Numb-DDB1-PI3KC3 may be a potential therapeutic target for AKI. Taken together, our data demonstrated that MeCP2 promoted cisplatin-induced renal tubular damage by facilitating the apoptotic process and inhibiting autophagy activity, which may be attributed to the epigenetic regulation of pro-apoptotic gene IRF8 by MeCP2.
- |||||||||| gemcitabine / Generic mfg.
Journal: Panax Notoginseng Saponins Promote Cell Death and Chemosensitivity in Pancreatic Cancer through the Apoptosis and Autophagy Pathways. (Pubmed Central) - Jan 20, 2022 Taken together, our data demonstrated that MeCP2 promoted cisplatin-induced renal tubular damage by facilitating the apoptotic process and inhibiting autophagy activity, which may be attributed to the epigenetic regulation of pro-apoptotic gene IRF8 by MeCP2. PNS may exhibit cytotoxicity and increase chemosensitivity of pancreatic cancer cells to Gem by inhibiting autophagy and inducing apoptosis, providing a new strategy and potential treatment option for pancreatic cancer.
- |||||||||| sirolimus / Generic mfg.
Preclinical, Journal: Profiling of Autophagy-Associated microRNAs in the Osteosarcoma Cell Line of U2OS. (Pubmed Central) - Jan 20, 2022 Our findings could facilitate the development of novel therapeutics for ischemic stroke. Together, we propose that the expressions of miR-3141, miR-4296, miR-133b, and miR-720 might exacerbate the pathogenesis of osteosarcoma; therefore, the suppression of these miRNAs with the loss-of-function approaches could be an appropriate strategy that worths testing in osteosarcoma.
- |||||||||| Journal: The neuroprotective effect of increased PINK1 expression following glutamate excitotoxicity in neuronal cells. (Pubmed Central) - Jan 20, 2022
Finally, we found that L-Glu could induce the expression of PINK1/Parkin/ LC3 in neurons at both mRNA and protein levels, while RAP could further increase their expression, and 3-MA decreased their expression. Taken together, PINK1 protects against L-Glu-induced neuronal injury by protecting mitochondrial function, and the potential protective mechanism may be closely related to the enhancement of mitophagy mediated by the PINK1/Parkin signaling pathway.
- |||||||||| Journal: Autophagy and pressure overload-induced cardiac hypertrophy. (Pubmed Central) - Jan 20, 2022
Cardiac hypertrophy may be alleviated through regulation of autophagy. This review aims to highlight the pathways and therapeutic targets identified in the prevention and treatment of cardiac hypertrophy by regulating autophagy.
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