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Interpreting High Levels of Unfolded Von Willebrand Factor in Patients with the Antiphospholipid Syndrome (Halls G-H (San Diego Convention Center)) - Nov 6, 2024 - Abstract #ASH2024ASH_6870;
Unfolded VWF levels were measured using a llama-derived single-domain antibody (VHH) directed against a cryptic epitope in the A1 domain of VWF, which is only exposed upon unfolding of VWF...For non-APS, non-thrombosis AID a 3.7-fold higher OR was found. These data suggest an additive effect of the auto-immune disease component and the thrombosis component of the APS pathology, resulting in a stronger association of high unfolded VWF levels and APS, although this needs to be confirmed in other populations.
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Journal: Sustained release of a human pd-l1 single-domain antibody using peptide-based hydrogels. (Pubmed Central) - Feb 19, 2024
Additionally, after encapsulation in the hydrogel and s.c. administration, a significantly extended systemic presence and tumor uptake of K2 was observed in mice bearing a melanoma tumor expressing human PD-L1. Altogether, this study describes how peptide hydrogels can be exploited to provide the sustained release of sdAbs, thereby potentially enhancing its clinical and therapeutic effects.
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Review, Journal: Promises and challenges of single-domain antibodies to control influenza. (Pubmed Central) - Jan 14, 2024
SdAbs directed against influenza A matrix protein 2 or its ectodomain have been reported, while no sdAbs have been described targeting the influenza B matrix protein 2. Known for their high specificity, ease of production and formatting, sdAb-based antivirals could be a major leap forward in influenza control.
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Journal: Safety assessment of fluorescently labeled anti-EGFR Nanobodies in healthy dogs. (Pubmed Central) - Oct 2, 2023
Images of the oral mucosa, acquired with a fluorescence imaging device (Fluobeam), suggest rapid clearance, which was in accordance with previous in vivo studies. These are the first results to indicate that 7D12-s775z is well tolerated in dogs and paves the way to conduct clinical trials in canine patients with EGFR-overexpressing spontaneous tumors.
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Journal: GlycoVHH: optimal sites for introducing N-glycans on the camelid VHH antibody scaffold and use for macrophage delivery. (Pubmed Central) - May 26, 2023
A VHH carrying predominantly a ManGlcNAc N-glycan at one of these preferred sites showed highly efficient, glycan-dependent uptake by Mf4/4 macrophages in vitro and by alveolar lung macrophages in vivo, illustrating one potential application of glyco-engineered VHHs: a glycan-based targeting approach for lung macrophage endolysosomal system delivery. The set of optimal artificial VHH N-glycosylation sites identified in this study can serve as a blueprint for targeted glyco-engineering of other VHHs, enabling site-specific functionalization through the rapidly expanding toolbox of synthetic glycobiology.
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Journal: Site-Specific Fluorescent Labeling, Single-Step Immunocytochemistry, and Delivery of Nanobodies into Living Cells. (Pubmed Central) - Feb 22, 2022
The smallest natural antibody fragments currently available are single-domain antibodies obtained from camelid species and sharks (variable new antigen receptors)...Also described are methods for delivery of fluorescent nanobodies into the cytoplasm of mammalian cells by photoporation, a very low-toxicity approach involving laser light and graphene quantum dots. The combined protocol embodies a novel route for studying protein function in living cells at high resolution.
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Clinical, Journal: Improving the yield of recalcitrant Nanobodies® by simple modifications to the standard protocol. (Pubmed Central) - Jan 28, 2022
Nanobodies are single-domain antibody constructs derived from the variable regions of heavy chain only (VH) camelid IgGs...The periplasmic extraction protocol was also changed to ensure efficient osmolysis, prevent both protein degradation and prevent downstream chelation of Ni ions during IMAC purification. Adoption of this protocol will lead to an improvement of the expression of Nanobodies in general, and specifically, those that are recalcitrant.
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Review, Journal: An Inside Job: Applications of Intracellular Single Domain Antibodies. (Pubmed Central) - Sep 11, 2021
In this review we provide a brief overview of recent advances in the field of intracellular single domain antibodies, focusing on their use as research tools and potential therapeutic applications. Special attention is given to the available methods that allow delivery of single domain antibodies into cells.
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[VIRTUAL] TEACHING AN OLD DOG NEW TRICKS: ACTIVITY-ON-TARGET INTERFERON TO TREAT T-ALL () - May 16, 2020 - Abstract #EHA2020EHA_628;
Mutated IFNa2Q124R, with a strongly reduced affinity for its receptor complex, was fused to single domain antibodies targeting cell-specific domains, which selectively restores the AcTaferon (AFN) activity in a cell-type specific manner...No synergism was observed between asparaginase and the indirect immune-mediated Clec9A-AFN anti-leukemic effect. Conclusion In conclusion, we have developed novel optimized immunocytokines as an off-the-shelve targeted immunotherapy for T-ALL.
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