- |||||||||| low molecular weight dextran sulfate (ILB) / TikoMed
Journal: Porous polymeric microparticles as an oral drug platform for effective ulcerative colitis treatment. (Pubmed Central) - Jul 29, 2020
Furthermore, oral administered porous MPs achieved statistically significantly better therapeutic outcomes against ulcerative colitis (UC) mouse model induced by dextran sulfate sodium, in comparison with non-porous MPs. These findings confirmed that porous MPs could be served as a promising platform for the treatment of UC via oral route.
- |||||||||| low molecular weight dextran sulfate (ILB) / TikoMed
Clinical, Journal: Switching to a Healthy Diet Prevents the Detrimental Effects of Western Diet in a Colitis-Associated Colorectal Cancer Model. (Pubmed Central) - Jul 29, 2020
We hypothesized that Western-style diet (WD) aggravates azoxymethane (AOM)/dextran sulfate sodium salt (DSS)-induced colitis-associated tumorigenesis and that switching to the standard AIN93G diet will ameliorate disease symptoms even after cancer initiation...The switch to the AIN93G diet ameliorated this effect, reflected by longer colons, fewer (p < 0.05) and smaller (p < 0.01) aberrant colonic crypt foci, comparable with the AIN group. Our results show that switching to a healthy diet, even after cancer initiation is able to revert the deleterious effect of the WD and could be an effective preventive strategy to reduce colitis symptoms and prevent tumorigenesis.
- |||||||||| low molecular weight dextran sulfate (ILB) / TikoMed
Preclinical, Journal: Inhibitory Effect of Steamed Soybean Wastewater Against DSS-Induced Intestinal Inflammation in Mice. (Pubmed Central) - Jul 28, 2020
It suggests that SSW could prevent intestinal inflammation in humans, although its efficacy should be verified through careful study design in humans. These findings have implications for enhancement of the value-added of SSW and for reduction of wastewater treatment costs incurred by the food industry.
- |||||||||| low molecular weight dextran sulfate (ILB) / TikoMed
Journal: Effect of Sishen Pill on Memory T Cells From Experimental Colitis Induced by Dextran Sulfate Sodium. (Pubmed Central) - Jul 28, 2020
Furthermore, SSP markedly inhibited activation of PI3K, Akt, phospho-Akt, Id2, T-bet, forkhead box O3a, Noxa, and C-myc proteins in the PI3K/Akt signaling pathway and activated Rictor, Raptor, tuberous sclerosis complex (TSC)1, TSC2, phospho-AMP-activated kinase (AMPK)-α, AMPK-α, eukaryotic translation initiation factor 4E-binding protein 2, kinesin family member 2a, and 70-kDa ribosomal protein S6 kinase. These results indicate that SSP effectively controls Tem cells in the peripheral blood to relieve experimental colitis induced by DSS, which were potentially related with inhibiting the PI3K/Akt signaling pathway.
- |||||||||| low molecular weight dextran sulfate (ILB) / TikoMed
Preclinical, Journal: β-Sitosterol improves experimental colitis in mice with a target against pathogenic bacteria. (Pubmed Central) - Jul 25, 2020
β-Sitosterol significantly reduces the weight loss, colon length, and alleviated microscopic appearances of colitis induced by dextran sulfate sodium...β-Sitosterol treatment to intestinal epithelial cells significantly increases expression of antimicrobial peptides and reduces survival of intracellular Salmonella typhimurium. These results showed the multiple effects of β-sitosterol against pathogenic bacteria for a novel approach to the treatment of colonic inflammation.
- |||||||||| low molecular weight dextran sulfate (ILB) / TikoMed
Journal: 5-Hydroxytryptamine (5-HT)-exacerbated DSS-induced colitis is associated with elevated NADPH oxidase expression in the colon. (Pubmed Central) - Jul 21, 2020
These results showed the multiple effects of β-sitosterol against pathogenic bacteria for a novel approach to the treatment of colonic inflammation. As 5-HT induced NOX1 and DUOX2 gene and protein expression in colon epithelial and HT-29 cells, NOX2 and NOX4 in the infiltrating leukocyte in mouse colon and in U-937 cells, the exacerbate colitis induced by combined 5-HT and DSS treatment might be relevant to increased NOX expression in mice colons.
- |||||||||| low molecular weight dextran sulfate (ILB) / TikoMed
Preclinical, Journal: Circadian Pharmacological Effects of Berberine on Chronic Colitis in Mice: Role of the Clock Component Rev-erbα. (Pubmed Central) - Jul 21, 2020
Chronic colitis was induced by feeding mice with dextran sulfate sodium in drinking water...In conclusion, circadian pharmacological effects of berberine on chronic colitis were mainly contributed by diurnal rhythms of both disease severity and Rev-erbα (as a drug target). The findings may have implications for chronotherapeutic practice on colitis or related diseases.
- |||||||||| low molecular weight dextran sulfate (ILB) / TikoMed
Preclinical, Journal: Endogenous murine microbiota member Faecalibaculum rodentium and its human homologue protect from intestinal tumour growth. (Pubmed Central) - Jul 21, 2020
Reconstitution of Apc or azoxymethane- and dextran sulfate sodium-treated mice with an isolate of F. rodentium (F...Both F. PB1 and H. biformis produced short-chain fatty acids that contributed to control protein acetylation and tumour cell proliferation by inhibiting calcineurin and NFATc3 activation in mouse and human settings. We have thus identified endogenous anti-tumourigenic bacterial strains with strong diagnostic, therapeutic and translational potential.
- |||||||||| low molecular weight dextran sulfate (ILB) / TikoMed
Preclinical, Journal: Effects of sDR5-Fc fusion protein on infant mice with ulcerative colitis via the TRAIL-DR5 pathway. (Pubmed Central) - Jul 19, 2020
To explore effects of the sDR5-Fc fusion protein on ulcerative colitis of infant mice via the TRAIL-DR5 pathway, 50 female mice were randomly divided into 5 groups, i.e., control group (group A), dextran sulfate sodium group (group B), hIgG group (group C), 10 mg/kg sDR5-Fc group (group D), and 20 mg/ kg sDR5-Fc group (group E)...Thus, sDR5-Fc could inhibit the activation of NLRP3 inflammasomes induced by TRAIL, thereby decreasing the apoptosis of NCM460 cells. In conclusion, the sDR5-Fc fusion protein could block the TRAIL-DR5 pathway to reduce the expression of NLRP3 inflammasomes, thereby improving ulcerative colitis.
- |||||||||| low molecular weight dextran sulfate (ILB) / TikoMed
[VIRTUAL] SETDB1 MAINTAINS INTESTINAL EPITHELIAL GENOME STABILITY AND PREVENTS INTESTINAL INFLAMMATION (Hall 2) - Jul 19, 2020 - Abstract #UEGW2020UEGW_1822;
Our study unveils a critical role of SETDB1 in maintenance of genome stability in IECs, and consequently intestinal epithelial homeostasis and prevention of intestinal inflammation. As certain rare variants in SETDB1 are over-represented in IBD patients, we continue to investigate if these variants can contribute to IBD pathogenesis, potentially in a monogenic manner.
- |||||||||| low molecular weight dextran sulfate (ILB) / TikoMed
Journal: Preventive effects of Korean red ginseng on experimentally induced colitis and colon carcinogenesis. (Pubmed Central) - Jul 17, 2020
In this study, we investigated effects of KRG on dextran sulfate sodium (DSS)-induced colitis and azoxymethane (AOM) plus DSS-induced colon carcinogenesis in mice...The up-regulation of COX-2, iNOS, c-Myc and Cyclin D1 by AOM plus DSS was attenuated in KRG fed mice which was associated with suppression of NF-κB and STAT3 activation. These results suggest that KRG is a potential candidate for chemoprevention of inflammation-associated cancer in the colon.
- |||||||||| low molecular weight dextran sulfate (ILB) / TikoMed
Preclinical, Journal: Anti-inflammatory effect of fucoxanthin on dextran sulfate sodium-induced colitis in mice. (Pubmed Central) - Jul 16, 2020
In addition, the DSS-induced overexpressions of inflammation-related molecules including COX-2 and NF-κB were significantly decreased in fucoxanthin-treated mice. These finding suggested that the use of fucoxanthin provides a new and attractive alternative to control UC.
- |||||||||| low molecular weight dextran sulfate (ILB) / TikoMed
Preclinical, Journal: FAM96A Protects Mice From Dextran Sulfate Sodium (DSS)-Induced Colitis by Preventing Microbial Dysbiosis. (Pubmed Central) - Jul 16, 2020
Taken together, our data indicate that FAM96A helps to maintain colonic homeostasis and protect against DSS-induced colitis by preventing gut microbial dysbiosis. This study used gene knockout animals to help to understand the in vivo effects of the Fam96a gene for the first time and provides new evidence regarding host-microbiota interactions.
- |||||||||| low molecular weight dextran sulfate (ILB) / TikoMed
Preclinical, Journal: Sodium Butyrate Alleviates Mouse Colitis by Regulating Gut Microbiota Dysbiosis. (Pubmed Central) - Jul 11, 2020
The present study aimed to elucidate the protective effects and underlying mechanisms of a short-chain fatty acid salt, sodium butyrate, on colonic inflammation induced by dextran sulfate sodium (DSS) in mice...This work verifies that sodium butyrate can improve mouse colitis via microbe-host interactions by regulating the microbial community. Taken together, the findings demonstrate that sodium butyrate shows great potential as a probiotic agent for ameliorating colitis.
- |||||||||| low molecular weight dextran sulfate (ILB) / TikoMed
Preclinical, Journal: Anti-Inflammatory Activity and Mechanism of Hydrostatin-SN1 From Hydrophis cyanocinctus in Interleukin-10 Knockout Mice. (Pubmed Central) - Jul 9, 2020
In our previous study, Hydrostatin-SN1, a bioactive peptide extracted from the Hydrophis cyanocinctus venom gland T7 phage display library, demonstrated anti-inflammatory activity in a dextran sulfate sodium-induced murine colitis model...Additionally, the positive expression rate of TNF-α in mice colon was decreased. Overall, our results suggest that Hydrostatin-SN1 has significant anti-inflammatory effects, both in vitro and in vivo.
- |||||||||| low molecular weight dextran sulfate (ILB) / TikoMed
Journal: Regulatory Effect of Lactobacillus brevis Bmb6 on Gut Barrier Functions in Experimental Colitis. (Pubmed Central) - Jul 8, 2020
In a 14-day dextran sulfate sodium (DSS)-induced colitis mouse model, treatment with L. brevis Bmb6 significantly decreased in the disease activity index score...Consistently, the gene expression of inflammatory cytokines (tumor necrosis factor-α and interferon-γ) was also suppressed in the Bmb6-treated mice. Hence, our findings suggest that suppression of inflammatory conditions enhanced expression of TJ protein, ZO-1, or vice versa, contributing to a colitis-ameliorating effect in L. brevis Bmb6.
- |||||||||| low molecular weight dextran sulfate (ILB) / TikoMed
[VIRTUAL] Scavenger Receptor-Mediated Uptake of DNAZyme-Gold Nanoparticle Conjugates in Alveolar Macrophages Using Two Photon Microscopy Analysis (ATS 2020 Virtual) - Jul 6, 2020 - Abstract #ATSI2020ATS-I_3660;
Methods : Using two-photon photoluminescence microscopy, we measured the emission intensity of DzNPs in murine alveolar macrophages (MH-S) treated for 24 hours with increasing doses of DzNP with and without increasing doses of the SR inhibitor dextran sulfate sodium (DSS)...DzNP uptake in alveolar macrophages is dose dependent with a Poisson distribution and is mediated by scavenger receptors. With spectral resolution around 170 nm, spacial localization of DzNP within the cell is possible with relative precision without the need for fluorescent labeling, thus offering greater optical versatility when fluorescent labeling is necessary to visualize other proteins or organelles of interest.
- |||||||||| low molecular weight dextran sulfate (ILB) / TikoMed, indomethacin / PLx Pharma Inc, Generic mfg.
Journal: Gastroprotective effects of the nonsaponin fraction of Korean Red Ginseng through cyclooxygenase-1 upregulation. (Pubmed Central) - Jul 5, 2020
In addition, NSF treatment in mice ameliorated the symptoms of gastrointestinal inflammation, including histological score, colon length, gastric juice pH, gastric wall thickness, and myeloperoxidase activity. These results suggest that NSF has gastroprotective effects on gastritis and colitis in in vivo mouse models through COX-1 upregulation.
- |||||||||| low molecular weight dextran sulfate (ILB) / TikoMed
Preclinical, Journal: Andrographolide inhibits inflammatory responses in LPS-stimulated macrophages and murine acute colitis through activating AMPK. (Pubmed Central) - Jul 4, 2020
In this study, we confirmed the inhibitory effect of Andro on inflammatory activity and studied its mechanism in depth to find potential anti-inflammatory targets of Andro using lipopolysaccharide (LPS)-induced macrophages in vitro and a dextran sulfate sodium (DSS)-induced mouse model of acute colitis in vivo...Moreover, Andro attenuated DSS-induced intestinal barrier dysfunction and inflammation by suppressing the NF-κB and MAPK pathways in colon tissues while activating the AMPK pathway. In conclusion, our study demonstrates that Andro effectively inhibits LPS-induced inflammatory responses via AMPK activation in macrophages, whereby Andro can ameliorate DSS-induced acute colitis in mice.
- |||||||||| low molecular weight dextran sulfate (ILB) / TikoMed, sulfasalazine / Generic mfg.
Journal: Combination of probiotics with different functions alleviate DSS-induced colitis by regulating intestinal microbiota, IL-10, and barrier function. (Pubmed Central) - Jul 4, 2020
The combined species, as well as the species individually, were tested and compared with sulfasalazine (SASP) and two Chinese herbal therapies...In addition, probiotic mixture significantly enhanced the expression of IL-10 and intestinal barrier function. These results suggest that a combination of multiple probiotics with different functions has synergistic effects and can restore the balance of interactions between microorganisms and immunological niches.
- |||||||||| low molecular weight dextran sulfate (ILB) / TikoMed
Journal: Enhancement of epithelial cell autophagy induced by sinensetin alleviates epithelial barrier dysfunction in colitis. (Pubmed Central) - Jul 2, 2020
These results suggest that a combination of multiple probiotics with different functions has synergistic effects and can restore the balance of interactions between microorganisms and immunological niches. Animal model of colitis were established by intracolonic administration of 2, 4, 6-trinitrobenzene sulfonic acid and oral treatment with dextran sulfate sodium...No abstract available
- |||||||||| low molecular weight dextran sulfate (ILB) / TikoMed, sulfasalazine / Generic mfg.
Preclinical, Journal: Effects of 6-Shogaol on Notch signaling pathway in colonic epithelial cells of ulcerative colitis mice (Pubmed Central) - Jul 1, 2020
Animal model of colitis were established by intracolonic administration of 2, 4, 6-trinitrobenzene sulfonic acid and oral treatment with dextran sulfate sodium...No abstract available 6-Shogaol can inhibit the over activation of Notch pathway and regulate the balance of differentiation between colonic epithelialabsorptive cell line and secretory cell line and repair damaged mucosal tissue.
- |||||||||| low molecular weight dextran sulfate (ILB) / TikoMed
Journal: MK2 Is Required for Neutrophil-Derived ROS Production and Inflammatory Bowel Disease. (Pubmed Central) - Jul 1, 2020
Here, we found that MAPK-activated protein kinase 2 (MK2) modulates ROS production and is required for dextran sulfate sodium (DSS)-induced IBD in the mouse model...Furthermore, upon treatment with formylated peptide N-formyl-methionyl-leucyl-phenylalanine (fMLF), the generation of ROS was attenuated in MK2-deficient neutrophils, in which the phosphorylation of Akt and p38 MAPK was also reduced. Collectively, these findings indicated that MK2 is required for neutrophil-derived ROS production and IBD, and MK2 and ROS are promising therapeutic targets for IBD.
- |||||||||| low molecular weight dextran sulfate (ILB) / TikoMed
Preclinical, Journal: Murine Appendectomy Model of Chronic Colitis Associated Colorectal Cancer by Precise Localization of Caecal Patch. (Pubmed Central) - Jun 28, 2020
This report introduces a facile protocol for caecal patch removal in mice followed by the chemical induction of chronic colitis-associated colorectal cancer using a combination of dextran sulfate sodium (DSS) and azoxymethane (AOM)...Histological results confirmed colonic inflammation and different degrees of adenocarcinoma. This model can be used for the study of the functional role of the appendix in maintaining gut microbiota homeostasis and pathogenesis of gut colitis and malignancies, as well as for the potential development of drug targeting therapies.
- |||||||||| low molecular weight dextran sulfate (ILB) / TikoMed
Preclinical, Journal: Sucralose Promotes Colitis-Associated Colorectal Cancer Risk in a Murine Model Along With Changes in Microbiota. (Pubmed Central) - Jun 27, 2020
Our study demonstrated that sucralose greatly aggravated dextran sulfate sodium (DSS)-induced colitis along with causing changes in gut microbiota, the gut barrier and impaired inactivation of digestive proteases mediated by deconjugated bilirubin...The results showed that sucralose caused significant increases in the number and size of AOM/DSS-induced colorectal tumors along with changes in other parameters such as body and spleen weight, pathological scores, mortality, fecal β-glucuronidase and digestive proteases, gut barrier molecules, gut microbiota, inflammatory cytokines and pathways (TNFα, IL-1β, IL-6, IL-10, and TLR4/Myd88/NF-κB signaling), and STAT3/VEGF tumor-associated signaling pathway molecules. These results suggest that sucralose may increase tumorigenesis along with dysbiosis of gut microbiota, impaired inactivation of digestive protease, damage to the gut barrier, and exacerbated inflammation.
- |||||||||| low molecular weight dextran sulfate (ILB) / TikoMed
Journal: Mevalonate promotes differentiation of regulatory T cells. (Pubmed Central) - Jun 26, 2020
Furthermore, we demonstrated that mevalonate treatment ameliorated dextran sulfate sodium (DSS)-induced colitis and resulted in an increased percentage of Tregs in vivo. Our results suggest that mevalonate enhanced Treg differentiation and ameliorated DSS colitis, indicating its potential for treatment of inflammatory diseases.
- |||||||||| low molecular weight dextran sulfate (ILB) / TikoMed
Journal: Quercetin-Induced miR-369-3p Suppresses Chronic Inflammatory Response Targeting C/EBP-β. (Pubmed Central) - Jun 26, 2020
Our results suggest that mevalonate enhanced Treg differentiation and ameliorated DSS colitis, indicating its potential for treatment of inflammatory diseases. These findings indicate that quercetin-induced miR-369-3p regulates the inflammatory cascade in chronic inflammatory response and present promising therapeutic implications.
- |||||||||| low molecular weight dextran sulfate (ILB) / TikoMed
Journal: EXOGENOUS LEPTIN REINFORCES INTESTINAL BARRIER FUNCTION AND PROTECTS FROM COLITIS. (Pubmed Central) - Jun 19, 2020
After 1 week, acute colitis was induced to C57BL/6 mice using dextran sulfate sodium (DSS) in drinking water...Leptin-treated mice displayed an increase in the expression of tight junction proteins and proliferative expression markers in colon, indicating a reinforcement in the mucosal barrier function induced by leptin administration. PEG-leptin treatment conferred protection to mice in the DSS model of colitis by reinforcing mucosal barrier function.
|
