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Preclinical, Journal: Protective Effects of Zerumbone on Colonic Tumorigenesis in Enterotoxigenic Bacteroides fragilis (ETBF)-Colonized AOM/DSS BALB/c Mice. (Pubmed Central) - Nov 19, 2020
In this study, we investigated the chemopreventive effects of oral administration of zerumbone in ETBF-colonized mice with azoxymethane (AOM)/dextran sulfate sodium (DSS)-induced tumorigenesis...Zerumbone treatment significantly inhibited colonic polyp numbers and prevented macroadenoma progression. Taken together, these findings suggest that oral treatment with zerumbone inhibited ETBF-promoted colon carcinogenesis in mice indicating that zerumbone could be employed as a promising protective agent against ETBF-mediated colorectal cancer.
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Journal: Carriers Based on Zein-Dextran Sulfate Sodium Binary Complex for the Sustained Delivery of Quercetin. (Pubmed Central) - Nov 18, 2020
The release mechanism of Que-loaded Zein/DSS composites is in accordance with the Higuchi model (Q = 0.0913t+0.1652, R = 0.953). Overall, nanoparticles based on Zein-DSS complexes stand out as an attractive carrier system of quercetin and the outcome could be extended to several bioactive compounds.
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Journal: Activation of PXR by alantolactone ameliorates DSS-induced experimental colitis via suppressing NF-κB signaling pathway. (Pubmed Central) - Nov 12, 2020
The present study demonstrated that ALA significantly ameliorated the clinical symptoms of dextran sulfate sodium (DSS)-induced mice colitis as determined by body weight loss, diarrhea, colon shortening, inflammatory infiltration and histological injury...Finally, molecular docking studies confirmed the binding affinity between hPXR-LBD and ALA. Collectively, the current study indicates a beneficial effect of ALA on experimental IBD possibly via PXR-mediated suppression of the NF-κB inflammatory signaling.
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Journal: Dietary Fiber Pectin Ameliorates Experimental Colitis in a Neutral Sugar Side Chain-Dependent Manner. (Pubmed Central) - Nov 12, 2020
Male C57BL/6 mice were fed a pectin-free diet, or a diet supplemented with characteristically high (5% orange pectin) or low (5% citrus pectin) side chain content for 10-14 days, and then administered 2,4,6-trinitrobenzene sulfonic acid or dextran sulfate sodium to induce colitis...IL-6 production from RAW264.7 cells stimulated with the toll-like receptor agonist Pam3CSK4 or lipopolysaccharide was suppressed by pre-treatment with orange pectin in vitro. Taken together, these results suggest that the side chains of pectin not only augment prebiotic effects but also directly regulate IL-6 production from intestinal host cells in a microbiota-independent fashion to attenuate colitis.
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Journal: Role of innate lymphoid cells in chronic colitis during anti-IL-17A therapy. (Pubmed Central) - Nov 12, 2020
An anti-IL-17A antibody, secukinumab, was also used to inhibit IL-17A function in the colitis model...In conclusion, RORγt ILCs may have an important role in the pathogenesis of chronic colitis in the absence of IL-17A. Blocking the function of IL-17A may upregulate Il6 and recruit RORγt ILCs in chronic colitis, thereby upregulating IL-22 and worsening the clinical outcomes of patients with Crohn's disease.
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Journal, IO Biomarker: Overexpression of miR-31 regulates TLR4/NF-κB signaling pathway and apoptotic protein in colitis model mice (Pubmed Central) - Nov 12, 2020
Objective: To investigate the effects of miR-31 on TLR4/NF-κB signaling pathway and apoptosis-related proteins in dextran sulfate sodium (DSS) induced mouse colon colitis...② In cell experiments, compared with the control group, the expression levels of NF-κB p65 and TLR4 protein in the over-expressed miR-31 group of HCT 116 cells were significantly increased (P＜0.05 or P＜0.01), the expressions of NF-κB p65 and TLR4 protein in miR-31 knockdown group were decreased (P＜0.05). miR-31 promotes the development of colitis by promoting TLR4/NF-κB signaling pathway and mediating apoptosis of intestinal epithelial cells.
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Preclinical, Journal: Berberine ameliorates colonic damage accompanied with the modulation of dysfunctional bacteria and functions in ulcerative colitis rats. (Pubmed Central) - Nov 7, 2020
UC rats were induced by dextran sulfate sodium (DSS) and administrated with berberine...Furthermore, the serum amino acids were regulated and showed high correlation with the gut microbiota after berberine treatment. In conclusion, the study confirms the effect of berberine on ameliorating the colonic damage and highlights some specific bacteria and relevant functions linked with berberine treatment, exploring the potential of gut microbiota as a diagnostic biomarker or a therapeutic target in UC treatment.
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Journal: Moxifloxacin based fluorescence imaging of intestinal goblet cells. (Pubmed Central) - Nov 6, 2020
Moxifloxacin based GC imaging was demonstrated not only by 3D microscopies but also by wide-field fluorescence microscopy, and intestinal GCs in the superficial region were imaged. Moxifloxacin based endomicroscopy has a potential for the application to human subjects by using FDA approved moxifloxacin.
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Preclinical, Journal: Intestinal inflammation increases convulsant activity and reduces antiepileptic drug efficacy in a mouse model of epilepsy. (Pubmed Central) - Nov 4, 2020
Intestinal inflammation was induced by dextran sulfate sodium (DSS) administration for 6 days...Furthermore, it appears that intestinal inflammation may reduce the antiepileptic effects of VPA, although we confirm that it decreases seizure threshold in this group. Therefore, we suggest that intestinal inflammation may represent a valid antiepileptic target which should also be considered as a participating factor to seizure incidence in susceptible patients and also could be relevant in reducing standard antiepileptic drug efficacy.
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Journal: Epithelial EP4 plays an essential role in maintaining homeostasis in colon. (Pubmed Central) - Nov 4, 2020
Our study is the first to demonstrate that epithelial EP4 loss resulted in potential "inflammatory" status under physiological conditions. These findings provided insights into the crucial role of epithelial PGE/EP4 axis in maintaining intestinal homeostasis.
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Journal: Modulation of gut microbiota by Ilex kudingcha improves dextran sulfate sodium-induced colitis. (Pubmed Central) - Nov 4, 2020
Furthermore, the correlation analysis suggested that KDCE could attenuate DSS-induced colitis which might be related to the alteration of gut microbiota. Therefore, the modulation of gut microbiota by KDCE might be a potential strategy for improving inflammation-driven diseases.
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Journal: Exosomes Derived From M2b Macrophages Attenuate DSS-Induced Colitis. (Pubmed Central) - Nov 2, 2020
Here, we analyze the roles of exosomes derived from M2a, M2b, and M2c macrophage phenotypes in dextran sulfate sodium (DSS)-induced colitis...The M2b macrophage exosomes exerted protective effects on DSS-induced colitis, mainly mediated by the CC chemokine 1 (CCL1)/CCR8 axis. These findings provide a novel approach for the treatment of IBD.
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Journal: Prolyl Hydroxylase Inhibition Mitigates Pouchitis. (Pubmed Central) - Oct 27, 2020
Cyclosporine treatment modulates butyrate uptake via the post-transcriptional upregulation of membrane MCT1 levels in IECs. Together, these results establish a strong therapeutic rationale for targeting PHD1 with small-molecule inhibitors in pouchitis after IPAA for UC.
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Preclinical, Journal: Adoptive transfer of Trichinella spiralis-activated macrophages can ameliorate both Th1- and Th2-activated inflammation in murine models. (Pubmed Central) - Oct 25, 2020
In a single application, we introduced macrophages obtained from T. spiralis-infected mice and T. spiralis ES protein-treated macrophages into mice tail veins before the induction of dextran sulfate sodium (DSS)-induced colitis, ovalbumin (OVA)-alum sensitization, and OVA challenge...Helminth infection and helminth ES proteins induce M2 macrophages. Adoptive transfer of macrophages obtained from helminth-infected mice and helminth ES protein-activated macrophages is an effective treatment for preventing and treating airway allergy in mice and is promising as a therapeutic for treating inflammatory diseases.
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Preclinical, Journal: A20 Restores Impaired Intestinal Permeability and Inhibits Th2 Response in Mice with Colitis. (Pubmed Central) - Oct 24, 2020
Adoptive transfer of macrophages obtained from helminth-infected mice and helminth ES protein-activated macrophages is an effective treatment for preventing and treating airway allergy in mice and is promising as a therapeutic for treating inflammatory diseases. A20 rectal treatment restores impaired intestinal permeability and inhibits activated Th2 cell response in mice with colitis.
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Journal: Gadolinium-Loaded Solid Lipid Nanoparticles for Colorectal Tumor in MR Colonography. (Pubmed Central) - Oct 23, 2020
Twenty mice received azoxymethane/dextran sulfate sodium (AOM/DSS) to induce adenocarcinoma of the colon and were divided into 4 groups, and 5 in per group...Enema of Gd-FITC-SLNs can provide marked enhancement of colorectal tumors quickly, and safer while intravenous injection of Gd-FITC-SLNs can provide a long-lasting enhancement of colorectal tumors in MR colonography. These findings present a potential clinical application of Gd-FITC-SLNs on MR colonography.
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Journal, IO Biomarker: Maresin 1 alleviates dextran sulfate sodium-induced ulcerative colitis by regulating NRF2 and TLR4/NF-kB signaling pathway. (Pubmed Central) - Oct 22, 2020
The current study may have promising therapeutic implications in the treatment of ulcerative colitis. MaR1 play a protective role in DSS-induced colitis by activating Nrf2 signaling and inactivating Nrf2-mediated TLR4/NF-κB signaling pathway, which mediate proinflammatory mediators and intestinal TJ proteins in rats, providing novel insights into the therapeutic strategy of colitis.
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Journal: Curculigoside inhibits ferroptosis in ulcerative colitis through the induction of GPX4. (Pubmed Central) - Oct 21, 2020
Herein, mice challenged with dextran sulfate sodium (DSS) were established and administrated with CUR for 7 days...Knockdown of GPX4 significantly blocked the protective effects of CUR on cell death, GSH and MDA contents as well as LDH activity in ferroptotic IEC-6 cells. Taken together, these findings suggest that CUR protects against ferroptosis in UC by the induction of GPX4, which presents a potential agent for UC treatment.
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Trial completion date, Trial primary completion date: Study to Evaluate Safety and Efficacy of IBsolvMIR in Islet Transplantation (clinicaltrials.gov) - Oct 12, 2020
P2, N=18, Not yet recruiting,
This paper was conducted to investigate the potent protective effects of RPE treatments on a mouse model of colitis and provided new ideas for the application of rape bee pollen. Trial completion date: Feb 2021 --> Oct 2021 | Trial primary completion date: Feb 2021 --> Oct 2021
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[VIRTUAL] STAT3 SIGNALING MAY MEDIATE PROTECTIVE EFFECT OF FXR AGONIST IN PARENTERAL NUTRITION ASSOCIATED CHOLESTASIS () - Oct 11, 2020 - Abstract #AASLD2020AASLD_65;
HepG2 cells and PNAC mouse model (treatment with oral dextran sulfate sodium [DSS] for 4 days followed by total PN for 14 days = DSS-PN mice [Nat Commun... These data demonstrate a previously undescribed mechanism, induction of STAT3 signaling, by which FXR agonists may be hepatoprotective in PNAC, and possibly other cholestatic liver diseases.
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Preclinical, Journal: Dietary resveratrol attenuated colitis and modulated gut microbiota in dextran sulfate sodium-treated mice. (Pubmed Central) - Oct 10, 2020
Pearson's correlation analysis indicated significant correlations between the relative levels of these pro-inflammatory cytokines and alterations of the gut microbiota. Our results demonstrated that dietary resveratrol attenuated the inflammatory status and alleviated gut microbiota dysbiosis in a colitis mouse model.
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Preclinical, Journal: Protective effect of Lachnum polysaccharide on dextran sulfate sodium-induced colitis in mice. (Pubmed Central) - Oct 9, 2020
In addition, LEP also inhibited (NOD)-like receptor family pyrin domain containing 3 (NLRP3) inflammasome activation, endoplasmic reticulum (ER) stress and oxidative/nitrosative stress induced by DSS. These results provided a scientific basis for LEP as a potential natural agent for protecting mice from DSS-induced IBD.
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Journal: Non-redundant functions of FAK and Pyk2 in intestinal epithelial repair. (Pubmed Central) - Oct 7, 2020
We previously showed that mice lacking FAK in the intestinal epithelium are phenotypically normal under homeostatic conditions but hypersensitive to experimental colitis induced by dextran sulfate sodium (DSS)...In contrast, mice lacking both FAK and Pyk2 develop spontaneous colitis with 100% penetrance by 4 weeks of age. Normal colonic phenotype and function are restored upon treatment of the double knockout mice with antibiotics, implicating commensal bacteria or bacterial products in the etiology of the spontaneous colitis exhibited by these mice.
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Preclinical, Journal: Diet Rich in Animal Protein Promotes Pro-inflammatory Macrophage Response and Exacerbates Colitis in Mice. (Pubmed Central) - Oct 1, 2020
We found that diet rich in animal protein (aHPD) exacerbates acute dextran sulfate sodium (DSS)-induced colitis while diet rich in plant protein (pHPD) does not...This treatment prevented aHPD induced increase in colonic Ly-6C pro-inflammatory monocytes, but the ratio of resident Ly-6C macrophages was not changed. These data show that the interactions between dietary protein of animal origin and gut microbiota increase sensitivity to intestinal inflammation by promoting pro-inflammatory response of monocytes.
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Preclinical, Journal: Impact of dietary manganese on experimental colitis in mice. (Pubmed Central) - Oct 1, 2020
Here, we provide evidence that Mn is critical for the maintenance of the intestinal barrier and that Mn deficiency exacerbates dextran sulfate sodium (DSS)-induced colitis in mice...Despite the well-appreciated roles of intestinal microbiota in driving inflammation in IBD, the gut microbiome composition was not altered by changes in dietary Mn. We conclude that Mn is necessary for proper maintenance of the intestinal barrier and provides protection against DSS-induced colon injury.
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Journal: Pharmacological activation of ERβ by arctigenin maintains the integrity of intestinal epithelial barrier in inflammatory bowel diseases. (Pubmed Central) - Oct 1, 2020
Arctigenin, the main active constituent in Fructus Arctii (a traditional Chinese medicine), has previously been found to attenuate colitis induced by dextran sulfate sodium (DSS) in mice...ERβ deletion in colons of mice with DSS-induced colitis resulted in a significant attenuation of the protective effect of arctigenin on the barrier integrity and colon inflammation. Arctigenin maintained the integrity of the intestinal epithelial barrier under IBD by upregulating the expression of tight junction proteins through the ERβ-MLCK/MLC pathway.
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Journal: The role of T cell trafficking in CTLA-4 blockade-induced gut immunopathology. (Pubmed Central) - Oct 1, 2020
Arctigenin maintained the integrity of the intestinal epithelial barrier under IBD by upregulating the expression of tight junction proteins through the ERβ-MLCK/MLC pathway. Our data reveals the fundamental mechanism underlying trafficking-blocking antibody therapy for CTLA-4 blockade-induced colitis and provide a caution in regard to apply trafficking-blocking antibody treatment under CTLA-4 blockade condition.
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Preclinical, Journal: Chronic Model of Inflammatory Bowel Disease in IL-10 Transgenic Mice: Evaluation with Ultrasound Molecular Imaging. (Pubmed Central) - Sep 30, 2020
Sustained colitis in IL-10 mice induced with piroxicam, TNBS or DSS, and in FVB mice induced with DSS, was not detected with USMI using MB, and this was verified by immunofluorescence staining for inflammation marker P-selectin. Thus, these models may not be appropriate for long-term monitoring of chronic colitis and subsequent treatment response with dual-selectin targeted USMI.
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