- |||||||||| fluoxetine / Generic mfg.
Social Isolation impacts depression, anxiety, and drug vulnerability in adolescent mice (SDCC Halls B-H) - Oct 10, 2022 - Abstract #Neuroscience2022NEUROSCIENCE_12873;
The results of this study will allow us to further understand the effect of adolescent social isolation and potential methods to ameliorate these effects, including the use of antidepressants like fluoxetine. This will be crucial for the development of new treatment strategies for adolescent stress.
- |||||||||| ketamine / Generic mfg., fluoxetine / Generic mfg.
Bdnf and trkb: key to psychedelic-induced plasticity (SDCC Halls B-H) - Oct 10, 2022 - Abstract #Neuroscience2022NEUROSCIENCE_12271;
In mice, M100907 completely blocks the head twitch response induced by psychedelics, but does not prevent the sustained antidepressant-like effects of LSD in the forced swimming test. All together, these results suggest that 5-HT2ARs mediate the head twitch response associated with hallucinogenic activity, but are detached from the BDNF/TRKB-dependent actions of psychedelics on plasticity and antidepressant-like behavioral responses.
- |||||||||| fluoxetine / Generic mfg.
A role for serotonin in the maturation of a glutamatergic synapse (SDCC Halls B-H) - Oct 10, 2022 - Abstract #Neuroscience2022NEUROSCIENCE_10773;
Consistent with this, we found increased expression of the phosphoinositide PIP3(phosphatidylinositol-(3,4,5)-trisphosphate) in the soma of tectal neurons following exposure to fluoxetine, and inhibiting PIP3 synthesis using the PI3-K inhibitor LY294002 abolished the potentiating effect of fluoxetine on retinotectal transmission. Given that PIP3 has been shown to be important for postsynaptic AMPA receptor clustering and long-term potentiation, our data indicate that endogenous serotonin plays a role in the maturation of retinotectal synapses through PI3-K signaling.
- |||||||||| fluoxetine / Generic mfg.
Contribution of the opioid system to the antidepressant effects of fluoxetine (SDCC Halls B-H) - Oct 10, 2022 - Abstract #Neuroscience2022NEUROSCIENCE_10209;
Given that PIP3 has been shown to be important for postsynaptic AMPA receptor clustering and long-term potentiation, our data indicate that endogenous serotonin plays a role in the maturation of retinotectal synapses through PI3-K signaling. These data indicate that the opioid system is involved in the antidepressant effects of fluoxetine, and this effect may be mediated through the upregulation of proenkephalin in a subpopulation of mature granule cells.
- |||||||||| A novel Post Traumatic Stress Disorder (PTSD) model in the rat (SDCC Halls B-H) - Oct 10, 2022 - Abstract #Neuroscience2022NEUROSCIENCE_10204;
PTSD rats exhibited significantly less social (interactive) activity by 21%. The model presented here is a simple, robust model of PTSD that should facilitate dissecting out various aspects of this disorder and provides a screening paradigm for new medical treatments.
- |||||||||| Withdrawn (SDCC Halls B-H) - Oct 10, 2022 - Abstract #Neuroscience2022NEUROSCIENCE_8838;
VIL may thus serve as an alternative SSRI with diminished addiction facilitating properties. Grant Support: FAPERJ Grant 202.944/2015, FAPERJ Grant 202.744/2019, FAPERJ Grant 201.432/2014, CNPq Grant 473324/2013-0, CNPq Grant 434093/2018-1, CNPq Grant 311487/2019-0, CNPq Grant 406436/2016-9, Alzheimer’s Association Grant AARG-D-615714, Serrapilheira Institute Grant R-2012-37967
- |||||||||| fluoxetine / Generic mfg.
Prenatal stress and fluoxetine exposure in BTBR mice alters the phenotype in male and female offspring (SDCC Halls B-H) - Oct 10, 2022 - Abstract #Neuroscience2022NEUROSCIENCE_7985;
The findings suggest that prenatal exposure to stress and/or an SSRI in a mouse model of autism can produce long-term behavioral alterations that are sex specific. The results suggest that prenatal experiences may interact with polygenic factors that contribute to the heterogeneity of symptoms and symptom severity in ASD.
- |||||||||| ketamine / Generic mfg., fluoxetine / Generic mfg.
Mast cells as novel modulators of brain serotonergic circuits driving anxiety (SDCC Halls B-H) - Oct 10, 2022 - Abstract #Neuroscience2022NEUROSCIENCE_7618;
Further, using immunohistochemistry, we found that sash mice show reduced serotonin+ cells in the dorsal raphe, and preliminary data suggest that the anxiety-like behavior in sash mice can be reduced with only a single IP injection of either 1mg/Kg ketamine (NMDAR antagonist) or 20mg/Kg fluoxetine (serotonin transporter inhibitor). Together, these results suggest that brain MCs play a fundamental role in preventing exaggerated anxiety responses through modulation of serotonergic/BDNF signaling, providing a novel mechanism underlying anxiety-like responses to stress.
- |||||||||| fluoxetine / Generic mfg.
Effects of Fluoxetine on Serotonin Transmission at the Egg-laying Behavior Circuit of C. elegans (SDCC Halls B-H) - Oct 10, 2022 - Abstract #Neuroscience2022NEUROSCIENCE_5905;
We predict that inhibition of egg laying by fluoxetine requires inhibitory serotonin receptors MOD-1 and SER-4, thus mutant animals lacking these receptors will be more active. Together, these results would support a model where long-term treatment with fluoxetine ultimately decreases serotonin release and signaling by inhibiting the activity of the serotonin releasing neurons.
- |||||||||| ketamine / Generic mfg.
Antidepressant properties of ketamine and psychedelics: A common cellular pathway? (SDCC Halls B-H) - Oct 10, 2022 - Abstract #Neuroscience2022NEUROSCIENCE_5185;
All experiments were repeated 4-6 times in triplicate.Antidepressants, psychedelics and ketamine all show an antidepressant biosignature, revealing amobilization of Gsα from lipid rafts and an increased signaling of GPCRs working through Gsα.Previous studies in postmortem brain and in platelets showed that this biomarker was enriched in lipidrafts from depressed subjects and reverted toward control values after effective treatment (as indicated byHAM-D). The similarities and differences in the actions of these drugs at the cellular level may lead to abetter molecular understanding of the distinct kinetic features of their actions.
- |||||||||| fluoxetine / Generic mfg.
Focal pharmacological manipulation of serotonin signaling in the amygdala alters primate social behavior (SDCC Halls B-H) - Oct 10, 2022 - Abstract #Neuroscience2022NEUROSCIENCE_4898;
Acute systemic administration of the selective serotonin reuptake inhibitor Fluoxetine decreases social interaction in rodents (Badgy et al., 2001; Payet et al., 2018)...Similar patterns of behavior were observed for total contact and reception of grooming. These results suggest differential contribution of particular serotonin receptors in the amygdala to social behavior in the nonhuman primate.
- |||||||||| citalopram / Generic mfg., fluoxetine / Generic mfg.
Sex differences in the gut microbiome of mice after chronic antidepressant treatment (SDCC Halls B-H) - Oct 10, 2022 - Abstract #Neuroscience2022NEUROSCIENCE_4738;
We are currently exploring changes in gut microbiota composition in male and female BALB/c mice after chronic treatment with fluoxetine or citalopram followed by microbiome profiling using 16s rRNA sequencing from fecal sample, including analysis of alpha (within community diversity) and beta (between communities) diversity across three-time points: baseline, midpoint, and after 28 days of SSRI administration to then compare between groups. The results of these experiments may help shed light on the possible mechanism of action of these SSRIs in the gut microbiome and the potential differences between males and females in therapy outcomes.
- |||||||||| ketamine / Generic mfg., fluoxetine / Generic mfg.
Antidepressants trigger the p75 proteolytic pathway in addition to inducing p75-dependent behavioral changes and brain plasticity (SDCC Halls B-H) - Oct 10, 2022 - Abstract #Neuroscience2022NEUROSCIENCE_4721;
The results of these experiments may help shed light on the possible mechanism of action of these SSRIs in the gut microbiome and the potential differences between males and females in therapy outcomes. Our data suggest that antidepressants as diverse as the typical fluoxetine and the fast-acting ketamine are able to trigger the p75 proteolytic pathway in vitro and that p75 signaling is indeed pertinent for their in vivo effects.
- |||||||||| fluoxetine / Generic mfg.
Chemical modification of the antidepressant fluoxetine to prevent its absorption through the intestinal wall (SDCC Halls B-H) - Oct 10, 2022 - Abstract #Neuroscience2022NEUROSCIENCE_4610;
Future studies will require characterization of binding properties of FLX+, SRT+ and PAX+ to the serotonin transporter, while at the same time testing the inability of these novel compounds to traverse the intestinal barrier. Finally, this chemical modification of SSRI’s should provide the pharmacological tools needed to test the hypothesis that drugs affecting the brain, also impact on gut microbes, and how the effect on gut symbionts contributes to the antidepressant effects of SSRI’s.
- |||||||||| fluoxetine / Generic mfg.
Antidepressant and anxiolytic properties of the extract and isolated compounds from Ziziphus abyssinica (SDCC Halls B-H) - Oct 10, 2022 - Abstract #Neuroscience2022NEUROSCIENCE_3503;
The anxiolytic and antidepressant properties exhibited by the crude extract and isolated compounds from the plant suggest that they could be further investigated as alternatives or complements to the already available drugs. Mechanisms mediating these effects are currently on-going in our laboratories.
- |||||||||| ketamine / Generic mfg., desipramine / Generic mfg., fluoxetine / Generic mfg.
Monoaminergic influence of the glutamatergic rapid-acting antidepressant RO-25-6981 and its analogs (SDCC Halls B-H) - Oct 10, 2022 - Abstract #Neuroscience2022NEUROSCIENCE_3500;
The ketamine-like glutamatergic antagonist RO-25-6981 exerts both rapid and sustained antidepressant-like activity...To assess antidepressant-like behaviors, the tail suspension test (TST) and locomotor activity (LA) tests were performed using RO-25-6981 and its analogs with traditional antidepressant drugs, including the serotonin selective reuptake inhibitor fluoxetine and the tricyclic antidepressant desipramine, as positive controls for monoaminergic reuptake activity...Taken together, these data suggest that the antidepressant-like activity of RO-25-6981 and its analogs does not correlate with the degree of NMDA receptor antagonism. Furthermore, these data point to serotonergic reuptake inhibition contributing to the overall antidepressant-like activity of RO-25-6981 in animal models of mood.
- |||||||||| ketamine / Generic mfg., pramipexole IR / Generic mfg., fluoxetine / Generic mfg.
Inhibition of group II metabotropic glutamate receptors for the treatment of anhedonia: evidence from behavioral and in vivo electrophysiological studies in rats (SDCC Halls B-H) - Oct 10, 2022 - Abstract #Neuroscience2022NEUROSCIENCE_3483;
These findings suggest that acute treatment with ketamine or inhibition of mGlu2/3 can normalize frontal low- and high-frequency EEG activity, suggesting that EEG activity may be used as a biomarker for treatment response. Further, inhibition of mGlu2/3 is as effective as ketamine in reversing reward learning impairments commonly observed in MDD and other psychiatric disorders characterized by anhedonia, but with fewer psychotomimetic effects, making it a potentially safer alternative treatment for anhedonia.
- |||||||||| ketamine / Generic mfg., fluoxetine / Generic mfg.
Methylone, a rapid-acting entactogen with robust antidepressant-like activity in the forced swim test (SDCC Halls B-H) - Oct 10, 2022 - Abstract #Neuroscience2022NEUROSCIENCE_3478;
We also explore effects of methylone on monoamine transporter binding, uptake, and release. Taken together, and consistent with the recent clinical case series, our results suggest that methylone may have clinical utility in the treatment of PTSD, MDD and other central nervous system disorders.
- |||||||||| fluoxetine / Generic mfg., escitalopram / Generic mfg.
Selective serotonin reuptake inhibitors within and near cells: Resolution at the compartmental and temporal levels via genetically encoded biosensors (SDCC Halls B-H) - Oct 10, 2022 - Abstract #Neuroscience2022NEUROSCIENCE_3476;
We interpret these data in light of previous reports that SSRIs both sequester within and perturb some membrane compartments and may reach SERT from the membrane phase. Although the time scale of our experiments is orders of magnitude slower than the “therapeutic lag” of SSRIs, the novel measurements presented here emphasize that pharmacokinetic properties of SSRIs, including their anomalously high volume of distribution, may play roles in both the therapeutic lag and the equally puzzling “antidepressant discontinuation syndrome”.
- |||||||||| fluoxetine / Generic mfg.
Histone serotonylation: a novel regulator of stress-induced neuroplasticity (SDCC Halls B-H) - Oct 10, 2022 - Abstract #Neuroscience2022NEUROSCIENCE_2803;
Defeated mice were 1) sacrificed 48h post-CSDS, 2) subjected to 30 days of fluoxetine vs. water treatment post-CSDS, or 3) underwent viral surgeries intra-DRN to block serotonylation pre-CSDS...Further RNA sequencing on virally infected tissue highlighted changes in serotonergic plasticity via knockdown of serotonylation, which may contribute importantly to antidepressant responses. In sum, this work implicates histone serotonylation in DRN as an important mediator of stress vulnerability via alterations in serotonergic signaling dynamics and related gene expression programs.
- |||||||||| fluoxetine / Generic mfg.
Psilocybin and PsyBio-11040 improve depressive-like behaviors in rats after chronic early life stress (SDCC Halls B-H) - Oct 10, 2022 - Abstract #Neuroscience2022NEUROSCIENCE_2223;
Therefore, to evaluate the antidepressant efficacy of these drugs, we treated 128 male and female Sprague Dawley rats (8 per group per sex per rearing condition) with psilocybin (1 mg/kg), PsyBio-11040 (1 mg/kg), fluoxetine (15 mg/kg), or vehicle intragastrically via gavage...Likewise, with preliminary results indicating no significant sex differences, they suggest that these compounds may be effective in both sexes. Funding for this research was provided by PsyBio Therapeutics, Inc.
- |||||||||| fluoxetine / Generic mfg.
Serotonin Modulates the Interaction between Prior Social Experience and Alcohol Sensitivity in Crayfish (SDCC 23) - Oct 10, 2022 - Abstract #Neuroscience2022NEUROSCIENCE_918;
Second, pre-treatment via bath application with fluoxetine, a selective serotonin reuptake inhibitor, facilitated the socially-mediated difference in EtOH sensitivity, further affirming interactions between the 5-HT system, social history, and EtOH sensitivity...One promising target for investigation of receptor changes is the LG circuit. Our ongoing work focuses on intracellular electrophysiology and neuropharmacology as well as molecular analyses such as western blotting and single-cell RNAseq of the LG neurons to confirm this hypothesis.
- |||||||||| Journal: Determination of 12 anti-obesity drugs in human plasma by a 96-well protein precipitation plate using HPLC-MS. (Pubmed Central) - Oct 7, 2022
The method was fully validated with an acceptable accuracy of 75.63-108.21%, matrix effect of 80.41-117.71% except for fenofibrate (76.07% at low concentration levels), and precision of 0.32-13.12%. Owing to the advantages of simple operation, high accuracy and sensitivity, this method is suitable for the rapid and simultaneous detection of 12 anti-obesity drugs in human plasma, providing support for clinically monitoring the development of adverse reactions and guiding the rational and appropriate use of weight-loss drugs for obese people.
- |||||||||| dizocilpine (MK801) / Merck (MSD)
Preclinical, Journal: Fluoxetine attenuates prepulse inhibition deficit induced by neonatal administration of MK-801 in mice. (Pubmed Central) - Oct 6, 2022
Our results showed fluoxetine attenuated PPI deficit and the decrease of cerebral BDNF expression in the MK-801-injected mice. These results suggest that fluoxetine can be used to treat sensorimotor gating deficit in a neurodevelopmental mouse model of schizophrenia, and the attenuating effect of fluoxetine on sensorimotor gating deficit may be related to fluoxetine's neuroprotective effect targeting on the modulation of cerebral BDNF.
- |||||||||| fluoxetine / Generic mfg.
Preclinical, Journal: The antidepressant effects and serum metabonomics of bifid triple viable capsule in a rat model of chronic unpredictable mild stress. (Pubmed Central) - Oct 4, 2022
Rats were randomly divided into Normal, CUMS model, fluoxetine hydrochloride (FLX), BTVCs, and FLX+BTVCs groups...The IPA demonstrated that endothelin-1 signalling and cyclic adenosine monophosphate response element binding protein (CREB) signalling in neurons may be involved in the development of depression. Our findings suggest that BTVCs can alleviate depressive-like behaviours, restore damage to the hippocampus in CUMS rats and regulate serum metabolism, which may be related to endothelin-1 signalling or CREB signalling in neurons.
- |||||||||| fluoxetine / Generic mfg.
Trial completion, Enrollment change: The Effect of G-DBT on the Patients With BN : A Multicenter Randomized Controlled Study (clinicaltrials.gov) - Oct 4, 2022
P=N/A, N=101, Completed,
Our findings suggest that BTVCs can alleviate depressive-like behaviours, restore damage to the hippocampus in CUMS rats and regulate serum metabolism, which may be related to endothelin-1 signalling or CREB signalling in neurons. Recruiting --> Completed | N=300 --> 101
- |||||||||| fluoxetine / Generic mfg.
Journal: The role of serotonergic signaling on phototactic and locomotor behavior in Daphnia magna. (Pubmed Central) - Sep 30, 2022
Exposure to exogenous serotonin re-established the phototactism and locomotor activity of TRH- individuals to those of the wild type but did not affect phototactic responses to fish kairomones. Unexpectedly, fluoxetine was able to modify locomotor activity and phototactic behavior against fish kairomones in TRH- individuals lacking serotonin, and also it increased the concentrations of acethylcholine and GABA in exposed animals, which support the argument that fluoxetine may also affect other neurological pathways.
- |||||||||| fluoxetine / Generic mfg.
Trial completion, Enrollment change: Fluoxetine to Reduce Intubation and Death After COVID19 Infection (clinicaltrials.gov) - Sep 30, 2022
P4, N=16, Completed,
Unexpectedly, fluoxetine was able to modify locomotor activity and phototactic behavior against fish kairomones in TRH- individuals lacking serotonin, and also it increased the concentrations of acethylcholine and GABA in exposed animals, which support the argument that fluoxetine may also affect other neurological pathways. Recruiting --> Completed | N=2000 --> 16
- |||||||||| fluoxetine / Generic mfg.
Journal: Octopamine mediates sugar relief from a chronic-stress-induced depression-like state in Drosophila. (Pubmed Central) - Sep 30, 2022
Stressed flies are relieved from the DLS by feeding the anti-depressant serotonin precursor 5-HTP or the selective serotonin reuptake inhibitor fluoxetine...In addition, neuronal-silencing experiments reveal that the serotonergic dorsal paired medial (DPM) neurons innervating the MB are essential for sugar relief. Conversely, thermogenetic or optogenetic activation of DPMs can replace sweet sensation, elucidating that serotonergic signaling from DPMs takes part in positively modulating DLS-related behavioral changes.
- |||||||||| Journal: Selective serotonin reuptake inhibitors and the risk of type 2 diabetes mellitus in youths. (Pubmed Central) - Sep 30, 2022
Conversely, thermogenetic or optogenetic activation of DPMs can replace sweet sensation, elucidating that serotonergic signaling from DPMs takes part in positively modulating DLS-related behavioral changes. T2DM risk did not differ between strong- and weak-affinity SSRIs, providing reassurance for clinicians when choosing between SSRIs in youths.
- |||||||||| Journal: Evaluation of eight psychoactive drugs used in Chinese cities by wastewater-based epidemiology. (Pubmed Central) - Sep 30, 2022
The concentrations of the psychoactive drugs diphenhydramine, fluoxetine, doxepin, imipramine, sulpiride, zolpidem, carbamazepine, and flunitrazepam in the wastewater were determined...We found associations between the use of the three drugs and age, occupation, and obesity, which are risk factors for mental disorders. The results showed that the monitoring of psychotropic drug using WBE has a certain reference value for public health care and for improving the understanding of mental disorders.
- |||||||||| sertraline / Generic mfg., fluoxetine / Generic mfg., escitalopram / Generic mfg.
Journal: Improved Serotonin Measurement with Fast-Scan Cyclic Voltammetry: Mitigating Fouling by SSRIs. (Pubmed Central) - Sep 30, 2022
Use of this waveform with bare CFMs alleviated the decrease in faradaic current, indicating decreased electrode fouling. Collectively, our results suggest that fouling considerations are important when designing FSCV experiments that employ SSRIs and that they can be overcome by using the appropriate waveform.
- |||||||||| fluoxetine / Generic mfg.
Journal: Synthesis and biological evaluation of (+)-paeoveitol derivatives as novel antidepressants. (Pubmed Central) - Sep 29, 2022
Moreover, compound 13 dose-dependently decreased the immobility of mice in the FST and showed an inverted U-shaped dose-effect, and the most efficacious dose (at 40 mg/kg) was comparable to fluoxetine (20 mg/kg) with a reduced immobility time of 29.2% and 34.5%, respectively...These findings suggest that compound 13 could be a potential antidepressant agent. Graphical abstract.
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