- |||||||||| doxycycline / Generic mfg.
Journal: High-Throughput Phenotypic Assay for Compounds That Influence Mitochondrial Health Using iPSC-Derived Human Neurons. (Pubmed Central) - Feb 23, 2022
Human induced pluripotent stem cells (iPSCs) with a stable genomic insertion of the neurogenin-2 transcription factor under the control of the TetOn promoter can be differentiated into excitatory human neurons (i3Neurons) within 3 days of exposure to doxycycline...The assay is rapid, with results obtained after a one-week-long i3Neuron culture or one month if neurons are co-cultured with astrocytes. This live-cell, mitochondrial phenotypic assay can be used for high-throughput screening or as an orthogonal assay for compounds obtained via other high-throughput screening campaigns.
- |||||||||| doxycycline / Generic mfg.
Journal: A DREaMR system to simplify combining mutations with rescue transgenes in Aedes aegypti. (Pubmed Central) - Feb 23, 2022
Using Drosophila white as the initial model, we demonstrated that introduction of a single insertion by CRISPR/Cas9 created a null mutation, a tagged rescue construct, which could be induced with doxycycline, and which allowed assessment of protein expression...Thus, this system avoids the need to perform genetic crosses to introduce an inducible rescue transgene in a mutant background, or to combine driver and reporter lines to examine expression of the targeted protein. We propose that DREaMR provides a system that can be applied to additional mosquito vectors as well as other organisms in which CRISPR/Cas9 is effective.
- |||||||||| doxycycline / Generic mfg.
Journal: Comprehensive analysis of prime editing outcomes in human embryonic stem cells. (Pubmed Central) - Feb 22, 2022
Here, we comprehensively tested prime editing in hPSCs by generating a doxycycline-inducible prime editing platform...Finally, whole-genome sequencing showed that, unlike the cytidine deaminase domain of cytosine base editors, the reverse transcriptase domain of a prime editor does not lead to guide RNA-independent off-target mutations in the genome. Our results demonstrate that prime editing in hPSCs has great potential for complementing previously developed CRISPR genome editing tools.
- |||||||||| doxycycline / Generic mfg.
Preclinical, Journal: Systemic and local effects of doxycycline and low-intensity laser treatment on periodontitis in rats. (Pubmed Central) - Feb 22, 2022
Our results illustrate that effective surveillance and strict biosecurity strategies should be taken to prevent the spread of multi-drug-resistant bacteria, and monitor the emergence of mobile genetic elements and integrons. The combination of DOX with LIL as SRP adjuvants was effective both systemically and locally for the treatment of experimental periodontitis in rats.
- |||||||||| icerguastat (IFB-088) / InFlectis
Bazedoxifene enhances early remyelination in the presence of inflammation (Exhibit Hall A) - Feb 20, 2022 - Abstract #ACTRIMSForum2022ACTRIMS_Forum_313;
We have showed that Sephin1 protected oligodendrocytes under inflammatory insults by prolonging a cytoprotective response - the integrated stress response (ISR)...Doxycycline (dox) inhibits IFN-γ expression in these mice, such that dox water was removed from experimental mice at the time of cuprizone exposure to trigger oligodendrocyte loss and demyelination in the presence of IFN-γ... Combination of the ISR modulator with OPC differentiating reagent facilitates the remyelination in the presence of inflammation.
- |||||||||| doxycycline / Generic mfg.
Preclinical, Journal: The Development of Sleep/Wake Disruption and Cataplexy as Hypocretin/Orexin Neurons Degenerate in Male vs. Female Orexin/tTA; TetO-DTA Mice. (Pubmed Central) - Feb 20, 2022
We compared the development of narcoleptic symptomatology in male vs. female orexin-tTA; TetO-DTA mice, a model in which Hcrt neuron degeneration can be initiated by removal of doxycycline (DOX) from the diet...During degeneration, both sexes also exhibited the "Delta State", characterized by sudden cessation of activity, high delta activity in the EEG, maintenance of muscle tone and posture, and the absence of phasic EMG activity. Since the phenotypes of the two sexes were indistinguishable by Week 6, we conclude that both sexes can be safely combined in future studies to reduce cost and animal use.
- |||||||||| ciprofloxacin oral / Generic mfg., doxycycline / Generic mfg., erythromycin / Generic mfg.
Journal: Antimicrobial susceptibility and virulence of Enterococcus spp. isolated from periodontitis-associated subgingival biofilm. (Pubmed Central) - Feb 19, 2022
Oral enterococci carry virulence genes and express resistance to some antibiotics commonly used in dentistry, such as ciprofloxacin and erythromycin. Specific subgingival taxa are associated with oral enterococci, suggesting they may interact with species of the dysbiotic periodontitis biofilm, constituting a potential source of factors to tissue destruction, antibiotic resistance dissemination and poor response to periodontal therapy.
- |||||||||| doxycycline / Generic mfg.
Journal: Activation of α7 nicotinic acetylcholine receptor ameliorates HIV-associated neurology and neuropathology. (Pubmed Central) - Feb 19, 2022
In the current study, we used doxycycline-inducible astrocyte-specific HIV Tat transgenic mice (iTat), a surrogate HAND model, and determined effects of PNU-125096, a positive allosteric modulator of α7 nicotinic acetylcholine receptor (α7 nAChR) on Tat-induced behavioural impairments and neuropathologies...Lastly, we used primary mouse cortical individual cultures and neuron-astrocytes co-cultures and in vivo staining of iTat mouse brain tissues and showed that glial activation was directly involved in the interplay among Tat neurotoxicity, α7 nAChR activation, and p38 MAPK signaling pathway. Taken together, these findings demonstrated for the first time that α7 nAChR activation led to protection against HAND and suggest that α7 nAChR modulator PNU-125096 hold significant promise for development of therapeutics for HAND.
- |||||||||| doxycycline / Generic mfg.
Journal: Continued Refinement of the Treatment for Light-Chain Cardiac Amyloidosis. (Pubmed Central) - Feb 19, 2022
Taken together, these findings demonstrated for the first time that α7 nAChR activation led to protection against HAND and suggest that α7 nAChR modulator PNU-125096 hold significant promise for development of therapeutics for HAND. No abstract available
- |||||||||| doxycycline / Generic mfg.
Journal: Adenovirus Co-Opts Neutrophilic Inflammation to Enhance Transduction of Epithelial Cells. (Pubmed Central) - Feb 19, 2022
In this manuscript, the molecular mechanisms behind PMN mediated enhancement of epithelial HAdV5 transduction are characterized using an MDCK cell line that stably expresses human CAR under a doxycycline inducible promoter (MDCK-CAR cells)...Inhibition of F-actin with cytochalasin D partially attenuates PMN mediated enhancement of HAdV transduction. Taken together, these findings suggest that HAdV5 can leverage innate immune responses to establish infections.
- |||||||||| ceftriaxone / Generic mfg.
Journal: Borreliosis and relapsing fever (Pubmed Central) - Feb 19, 2022
The mortality Rate varies from 2 to 5 % depending on the Borrelia Species involved. The outcome is usually good after treatment.
- |||||||||| Xalkori (crizotinib) / Pfizer
A Ping Pong Ball in My Airway: An Uncommon Endotracheal Tumor (Room 207-208 (South Building, Level 2), Moscone Center) - Feb 19, 2022 - Abstract #ATS2022ATS_6187;
ALK receptor tyrosine kinase rearrangements are present in up to 50% of IMTs, and competitive ALK inhibitors like crizotinib have shown utility in patients with metastatic or unresectable disease. Our case highlights an uncommon tumor type in the setting of progressive respiratory symptoms refractory to medical therapy, demonstrates successful endobronchial management, and suggests watchful waiting for optimization prior to surgical resection can be effectively performed in this low grade tumor.
- |||||||||| doxycycline / Generic mfg.
Wnt5a Promotes Alveolar Type 1 Cell Differentiation During Lung Development (Room 2022/2024 (West Building, Level 2), Moscone Center) - Feb 19, 2022 - Abstract #ATS2022ATS_6156;
The Wnt5a anti-AT2 regulatory pathway is associated with suppressing canonical Wnt signaling. Importantly, the Wnt5a pro-AT1 regulatory pathway is evolutionarily conserved between mouse and human and may act independent of canonical Wnt signaling.
- |||||||||| doxycycline / Generic mfg.
Sporadic Lymphangioleiomyomatosis Without a TSC1 or TSC2 Mutation (Room 9-10 (South Building, Exhibition Level), Moscone Center) - Feb 19, 2022 - Abstract #ATS2022ATS_5680;
Due to recurrent pneumothorax resistant to chest tube management, she underwent left video-assisted thorascopic surgery (VATS) and doxycycline pleurodesis...Our patient had lymphangioleiomyomatosis, one pathologically angiomyolipoma and multiple renal cysts which did not meet the diagnostic criteria for TSC. To the best of our knowledge, she represents an unusual case of sporadic LAM with additional findings of angiomyolipoma and ADPKD without a TSC1 or TSC2 mutation on conventional genetic testing.
- |||||||||| Ninlaro (ixazomib) / Takeda
Disseminated Exophiala Infection in an Immunocompromised Patient (Room 203-204 (South Building, Level 2), Moscone Center) - Feb 19, 2022 - Abstract #ATS2022ATS_5380;
Original fungal culture from the first admission resulted as an Exophiala species, and ambisome was added to voriconazole...Discussion Exophiala infection is a rare diagnosis but is being identified more frequently, likely related to increases in immunosuppression to facilitate solid organ transplantation. Even though disseminated Exophiala carries a poor prognosis, it must be considered in these patients to avoid treatment delays and reduce mortality.
- |||||||||| Rituxan (rituximab) / Biogen, Zenyaku Kogyo, Roche
Tick or Tack? Recurrence of Pulmonary Mucosa-Associated Lymphoid Tissue (MALT) Lymphoma Following Lyme Disease (Area I, Hall F (North Building, Exhibition Level), Moscone Center) - Feb 19, 2022 - Abstract #ATS2022ATS_5047;
It has been well-studied that marginal zone lymphomas are associated with chronic stimulation of antigens of microbial origin. Although no causative agent has been associated with pulmonary MALT lymphoma, this case is interesting in that we demonstrate the recurrence of pulmonary MALT lymphoma following diagnosis of Lyme disease.
- |||||||||| Sarclisa (isatuximab-irfc) / Sanofi
Congo Red Effusions: A Rare Cause of Pleural Disease (Area I, Hall F (North Building, Exhibition Level), Moscone Center) - Feb 19, 2022 - Abstract #ATS2022ATS_5030;
One must consider this when a patient presents with signs of a plasma cell dyscrasia and pleural effusions refractory to aggressive diuresis and large volume thoracentesis. Biopsy is generally necessary for confirmative testing.
- |||||||||| doxycycline / Generic mfg.
Pneumonic Tularemia: A Case Presentation (Area C, Hall F (North Building, Exhibition Level), Moscone Center) - Feb 19, 2022 - Abstract #ATS2022ATS_4787;
Patients with prolonged untreated tularemia can develop renal failure, sepsis, and rhabdomyolysis. Tularemia should be suspected in patients with risk factors for disease exposure and who develop an abrupt onset of systemic symptoms including fever and malaise.
- |||||||||| prednisone / Generic mfg., trimethoprim/sulfamethoxazole / Generic mfg., doxycycline / Generic mfg.
Nocardia Farcinica in a Patient with Silicosis Presenting as a Pseudo-Malignancy (Area C, Hall F (North Building, Exhibition Level), Moscone Center) - Feb 19, 2022 - Abstract #ATS2022ATS_4764;
However, final report 25 days later grew Nocardia farcinicia, and he was placed back on Bactrim...Chronic corticosteroids concomitantly reduce the number of macrophages in the alveoli and causes macrophage dysfunction (3). Hence, our patient was highly susceptible to pulmonary Nocardiosis due to multiple factors affecting macrophage function.
- |||||||||| Rituxan (rituximab) / Biogen, Zenyaku Kogyo, Roche
A Rare Case of Hemophagocytic Lymphohystiocytosis Secondary to Severe Epstein Barr Infection in an Immunocompetent Individual (Area A, Hall F (North Building, Exhibition Level), Moscone Center) - Feb 19, 2022 - Abstract #ATS2022ATS_4492;
Etiology is familial children but secondary in adulthood, where triggers include viral infection, cancers.Case presentation: A 60-year-old immunocompetent female with past medical history of hypertension, asthma, deep vein thrombosis on warfarin, hypothyroidism, and hyperlipidemia who presented due to fatigue and encephalopathy...She completed two separate month treatments with doxycycline for Lyme disease (despite negative serology) but had continued intermittent symptoms...She was empirically treated with antimicrobials, Dexamethasone 10 mg/m2 body surface area and etoposide...EBV serology was completed and was positive with viral load exceeding 5 million, thus rituximab was empirically started.She continued to have mucosal bleeding with increasing amounts of frank blood being passed from her ET tube...Upon removal of the bronchoscope, she promptly arrested, with no attempt at resuscitation made on the second arrest.Summary: Described is a rare case of HLH that was secondary to severe EBV infection masquerading as Lyme disease in an immunocompetent individual. Even with rapid initiation of empiric treatment and supportive measures, she expired in the setting of severe coagulopathy leading to respiratory compromise secondary to endobronchial hemorrhage.Figure 1: Bone marrow aspirate.
- |||||||||| Xifaxan (rifaximin) / Alfa Wassermann, Bausch Health, Norgine, ASKA Pharma
A Case of Acute Encephalopathy Following Lung Transplantation: Ureaplasma Induced Hyperammonemia Syndrome (Area J, Hall F (North Building, Exhibition Level), Moscone Center) - Feb 19, 2022 - Abstract #ATS2022ATS_4488;
Cerebral edema and seizure management (osmolar therapy, induced hypothermia, anti-epileptics) may be indicated.Hyperammonemia associated with ureaplasma infection should be high in the differential diagnosis of transplant patients with altered mental status. Early recognition and a multimodal treatment strategy can potentially lead to good outcomes.
- |||||||||| What's Abuzz? Encephalopathy in a Transplant Recipient (Area J, Hall F (North Building, Exhibition Level), Moscone Center) - Feb 19, 2022 - Abstract #ATS2022ATS_4486;
He was on fluconazole for coccidioidomycosis prophylaxis...He was started on empiric meningitis treatment with ampicillin, vancomycin, cefepime, and acyclovir, and was given doxycycline for atypical coverage...The second is management of a critically ill patient with meningitis and myasthenia gravis, since multiple agents for empiric therapy have been associated with worsening of or precipitating myasthenic crisis. Finally, supportive care is the mainstay of the management of WNV encephalitis and IVIG and adjustments in immunosuppression is controversial.
- |||||||||| ceftriaxone / Generic mfg., doxycycline / Generic mfg.
Chest X-ray Unremarkable: A Case of a "Late Blooming" Empyema (Area J, Hall F (North Building, Exhibition Level), Moscone Center) - Feb 19, 2022 - Abstract #ATS2022ATS_4454;
High cervical spine injuries (C1-C4) have been associated with an increased incidence of respiratory infections, likely due to the impairment of nerves innervating the diaphragm and oropharynx. While various modalities (POCUS and CT scan) can suggest the type of effusion by the presence/absence of certain features, it is important to keep empyema on the differential diagnosis and promptly perform a thoracentesis once pleural fluid is identified.
- |||||||||| dexamethasone / Generic mfg., doxycycline / Generic mfg.
Hemophagocytic Lymphohistiocytosis-Associated Stress Cardiomyopathy (Area G, Hall F (North Building, Exhibition Level), Moscone Center) - Feb 19, 2022 - Abstract #ATS2022ATS_2757;
Our patient’s LV function recovered after treatment of HLH and initiation of GDMT. Cardiac function should be assessed early in patients suspected to have HLH.
- |||||||||| Cryptogenic Organizing Pneumonia Due to Mycoplasma (Area B, Hall F (North Building, Exhibition Level), Moscone Center) - Feb 19, 2022 - Abstract #ATS2022ATS_2686;
She was treated with IV methylprednisolone, IV ceftriaxone, and doxycycline...The patient was discharged on tapering doses of steroids and levofloxacin to complete 14 days of antibiotics...Although open lung biopsy is considered the best approach for diagnosis, BAL with TBLB has a positive predictive value of around 95%. Symptomatic improvement in such patients is quite impressive with steroids with complete recovery in most of the patients.
- |||||||||| doxycycline / Generic mfg.
Common Variable Immunodeficiency Presenting as Persistent Pneumonia with Pulmonary Mass (Area I, Hall F (North Building, Exhibition Level), Moscone Center) - Feb 19, 2022 - Abstract #ATS2022ATS_2588;
Chest x-ray at that time revealed a left sided patchy opacity, and the patient was discharged on a course of doxycycline...This is in part due to a wide variety of clinical manifestations and range of ages affected. Due to its variable presentation, CVID should be considered in cases of suspected lymphoma.
- |||||||||| doxycycline / Generic mfg.
CYB5R3 Deficiency Imparts Dysfunctional Phenotype on Type II Alveolar Epithelial Cells in Mice (Room 212-214 (South Building, Level 2), Moscone Center) - Feb 19, 2022 - Abstract #ATS2022ATS_2114;
Our preliminary data suggest that CYB5R3 is critical for controlling mitochondrial health, lipid metabolism, inflammation, lineage program and overall homeostasis of AECII. Deficiency in expression of CYB5R3 in AECII from IPF lungs might serve as a basis for AECII dysfunction and reduced resilience to lung fibrosis.
- |||||||||| 19-Year-Old with Complete Lung Collapse, Respiratory Failure, and Septic Shock (Area H, Hall F (North Building, Exhibition Level), Moscone Center) - Feb 19, 2022 - Abstract #ATS2022ATS_1732;
Herein we present a patient with complete collapse of the right lung complicated with post-obstructive necrotizing pneumonia and septic shock secondary to an endobronchial carcinoid tumor causing central airway obstruction.CASE DESCRIPTION: A 19-year-old male with a history of asthma managed with albuterol and montelukast presented with fever, acute hypoxic respiratory failure, and septic shock to a local emergency room...He was started on IV fluids, vasopressors, oxygen supplementation, and empiric vancomycin, piperacillin-tazobactam, and doxycycline...Bronchoscopic biopsies are usually diagnostic, and imaging modalities such as CT scan can aid in establishing the diagnosis. As both asthma and endobronchial tumors present with obstructive physiology, the diagnosis of such neoplasms can be delayed in patients with history of asthma.
- |||||||||| Actemra IV (tocilizumab) / Roche, JW Pharma
When It's Not a Contaminant: A Case of Corynebacterium Infection (Area K, Hall F (North Building, Exhibition Level), Moscone Center) - Feb 19, 2022 - Abstract #ATS2022ATS_1475;
His underlying COVID was treated with ten days dexamethasone, two days methylprednisolone, and one dose tocilizumab...Sensitivities eventually returned, showing corynebacterium amycolatum resistant to clindamycin and erythromycin, intermediate to ceftriaxone and penicillin, and susceptible to linezolid, vancomycin, doxycycline, and Bactrim...Increased recognition of the pathogenicity of corynebacterium infections and understanding their unpredictable antibiotic susceptibilities is imperative to effectively treat patients and prevent additional infectious complications. While historically considered a contaminant, corynebacteria may present a serious infectious threat and should be approached accordingly.
- |||||||||| doxycycline / Generic mfg.
Weil Syndrome with Multiorgan Failure (Area K, Hall F (North Building, Exhibition Level), Moscone Center) - Feb 19, 2022 - Abstract #ATS2022ATS_1216;
He was empirically started on Doxycycline...Weil syndrome include multiorgan failure with renal dysfunction, bleeding disorders, lung damage, hepatosplenomegaly and rarely can include gastrointestinal bleeding, subarachnoid bleeding, myocarditis etc. Preventive antibiotic therapy once there is high suspicion for infection can reduce mortality as antibody appear in the serum in day 6-12th only. This case brings to light that is extremely important to treat empirically if the diagnosis is unknown to save lives!
- |||||||||| Lyme Carditis: An Unlikely Complication from a Usual Suspect (Area K, Hall F (North Building, Exhibition Level), Moscone Center) - Feb 19, 2022 - Abstract #ATS2022ATS_1211;
Lyme serologies returned positive and empiric antibiotics for meningioencephalitis including doxycycline, vancomycin, ceftriaxone, ampicillin and acyclovir started...Following extubation, tremors worsened despite increasing doses of gabapentin, antiepileptic and benzodiazepines...Centers for Disease Control and Prevention. Retrieved October 20, 2021, from https://www.cdc.gov/lyme/treatment/lymecarditis.html.
- |||||||||| atovaquone/proguanil / Generic mfg., doxycycline / Generic mfg., artemether/lumefantrine / Generic mfg.
A Case of Resistant Plasmodium Falciparum Infection Confounded by Rocky Mountain Spotted Fever? (Area K, Hall F (North Building, Exhibition Level), Moscone Center) - Feb 19, 2022 - Abstract #ATS2022ATS_1206;
This case represents an unusual persistence of Plasmodium falciparum infection despite multiple, appropriate antiparasitic treatment courses and supposed chemoprophylaxis in a patient who traveled to an endemic area. Further confounding the infectious workup, we incidentally found positive serology to Rocky Mountain spotted fever.
- |||||||||| Isentress (raltegravir) / Merck (MSD)
Spontaneous Tension Pneumothorax as the First Sign of HIV /AIDS (Area K, Hall F (North Building, Exhibition Level), Moscone Center) - Feb 19, 2022 - Abstract #ATS2022ATS_1205;
Despite negative BAL and sputum cultures our patient was treated with antibiotics targeting the main suspected organisms. Spontaneous pneumothorax due to infection can be difficult to treat due to underlying necrotizing tissue that prevents lung re-expansion with a high recurrence rate.
- |||||||||| Leptospirosis: The Underdiagnosed Catastrophe (Area K, Hall F (North Building, Exhibition Level), Moscone Center) - Feb 19, 2022 - Abstract #ATS2022ATS_1198;
He was admitted to MICU for worsening respiratory status and started empirically on Vancomycin and Piperacillin-tazobactam which was later switched to Cefepime on Day-3 to complete a seven-day course...Antibiotics were restarted with Doxycycline and Ceftriaxone...Severe pulmonary manifestations with alveolar hemorrhage is a serious complication that can result in ARDS, multi-organ failure, and death. High clinical suspicion supported by exposure history should prompt empiric coverage while confirmatory testing is pending.
- |||||||||| prednisone / Generic mfg., trimethoprim/sulfamethoxazole / Generic mfg., doxycycline / Generic mfg.
Drug and a Tick- A Rare Case of Drug Induced Hemolytic Anemia in a Patient with Spotted Fever Rickettsiosis (Area G, Hall F (North Building, Exhibition Level), Moscone Center) - Feb 19, 2022 - Abstract #ATS2022ATS_1174;
DIHA is a rare but serious complication of drug treatment...Direct antiglobulin tests may or may not be positive but are often positive in patients with DIHA from TMP-SMX. Treatment involves discontinuing the offending drug, and often responds to high dose steroids .
- |||||||||| Antibiotic Usage in Patients Experiencing Readmissions for Acute Exacerbations of Chronic Obstructive Pulmonary Disease (Area H, Hall F (North Building, Exhibition Level), Moscone Center) - Feb 19, 2022 - Abstract #ATS2022ATS_1055;
GOLD guidelines allow for various antibiotic choices (e.g., aminopenicillin with clavulanic acid, macrolide or tetracycline) and hospitalized patients with AECOPD receive a variety of antibiotics...Of the 31 patients who received azithromycin during their index admission, 51.6% had 1 or more readmissions within 60 days compared with 55.5% of 45 patients who received levofloxacin and 59.5% of 42 patients who received doxycycline (p=0.80 by Chi-square)...Patients received the same antibiotics when readmitted within 60 days, occasionally over multiple readmissions. Antibiotic selection did not influence outcomes in patient’s frequently hospitalized for AECOPD, but specific guidelines for antibiotic usage are needed for these patients.
- |||||||||| Tecentriq (atezolizumab) / Roche
Immune Checkpoint Inhibitor-Induced Interstitial Lung Disease Mistaken for Atypical Pneumonia (Area F, Hall F (North Building, Exhibition Level), Moscone Center) - Feb 19, 2022 - Abstract #ATS2022ATS_1007;
It is imperative to keep ICI-ILD in the list of differentials for patients with acute onset shortness of breath while receiving Atezolizumab therapy. Early recognition of this clinical entity can allow for timely management, reduce mortality, and improve long term outcomes.
- |||||||||| Acute Eosinophilic Pneumonia Caused by Cigar Smoking (Room 3009/3011 (West Building, Level 3), Moscone Center) - Feb 19, 2022 - Abstract #ATS2022ATS_708;
The patient was provided supplemental oxygen via nasal cannula and started on ceftriaxone and azithromycin for community acquired pneumonia...Antibiotic coverage was broadened to vancomycin, piperacillin-tazobactam, doxycycline and levofloxacin... AEP is a rare cause of acute respiratory failure that can be confidently diagnosed with careful history, a constellation of symptoms and signs, and BAL eosinophilia (>20-25%).
- |||||||||| doxycycline / Generic mfg.
Journal: The phosphatase PRL-3 affects intestinal homeostasis by altering the crypt cell composition. (Pubmed Central) - Feb 16, 2022
Here, we employ a doxycycline-inducible PRL-3 mouse strain to show that aberrant PRL-3 expression within a non-cancerous background leads to the death of Lgr5+ ISCs and to Paneth cell expansion...The impaired intestinal organoid formation was rescued by a PRL inhibitor, providing a functional link to the observed phenotypes. These results demonstrate that elevated PRL-3 phosphatase activity in healthy intestinal epithelium impairs intestinal cell homeostasis, which correlates this cellular mechanism of tumor onset with PRL-3-mediated higher susceptibility to tumor formation upon inflammatory or mutational events.Key messages• Transgenic mice homozygous for PRL-3 overexpression die early.• PRL-3 heterozygous mice display disrupted intestinal self-renewal capacity.• PRL-3 overexpression alone does not induce tumorigenesis in the mouse intestine.• PRL-3 activity leads to the death of Lgr5+ ISCs and Paneth cell expansion.• Impairment of cell homeostasis correlates PRL-3 action with tumor onset mechanisms.
|
