- |||||||||| doxycycline / Generic mfg.
Successful introduction of a point mutation into the genome of a primary colon cancer cell line using CRISPR base editing technology (Room A2) - Nov 30, 2022 - Abstract #DKK2022DKK_2308;
FACS detected 15% GFP positive cells using non-induced sgRNA three days after transduction and up to 5% GFP positive cells were found using an inducible sgRNA after three days of induction with doxycycline...High virus concentration and the use of a non-inducible sgRNA vector are potential important parameters for efficient editing. This strategy should be useful to study the biology of oncogenic mutations under the control of its endogenous promoter with- out the need for classical transgenic animal technology.
- |||||||||| doxycycline / Generic mfg., tetracycline / Generic mfg.
Identification of ATF4 As a Key Upstream Regulator of Acute Myeloid Leukemia Cell Metabolism () - Nov 29, 2022 - Abstract #ASH2022ASH_7322;
This suggests that ATF4 is broadly deregulated in AML and interventions targeting this pathway may be broadly applicable.To better understand the molecular role of ATF4 in AML cell biology, we engineered multiple human AML cells lines (NOMO1, MV4-11, and OCI-AML3) to express control (shNT) or shRNAs targeting ATF4 under the regulation of a tetracycline-inducible promoter...To identify downstream gene targets of ATF4 in AML, we carried out RNA-sequencing analysis in NOMO1 cells at a time point where we observe maximal knockdown of ATF4 protein (24 hours post-doxycycline [DOX] treatment) but prior to the evolution of cellular changes (~60-72 hours post-DOX)...Our results thus far have shown that ATF4 could be a potential broadly applicable therapeutic target. Further investigation is needed through stable-isotope tracing metabolomics to understand the impact ATF4 has on these amino acid metabolic pathways, which are currently ongoing; as well as to understand the direct effects ATF4 regulation of tRNA aminoacylation has on changes in the proteome, which we are currently investigating through quantitative proteomics using stable isotope labeling by amino acids in cell culture (SILAC).
- |||||||||| arsenic trioxide / Generic mfg., doxycycline / Generic mfg.
Targeting Interferon Pathway in TBLR1-RARa-Driven Promyelocytic Leukemia in the Context of ATRA and ATO Unresponsiveness () - Nov 29, 2022 - Abstract #ASH2022ASH_7164;
In TR-driven APL, despite the unresponsiveness of ATO, we may utilize STING-IFN pathway agonist to upregulate PML expression, which may obliterate self-renewal independent of ATO response triggered by initial PML nuclear body disruption observed in PR-driven APL, providing therapeutic implications for APL driven by rare RARα fusion genes. Our study aims to gain further understanding of TR-driven APL and integrate insights into principles underlying leukemogenesis mediated by rare RARα fusion genes, which may benefit such a therapy-resistant population and enable APL to be a bona fide curable leukemia.
- |||||||||| Overwhelming Intravascular Hemolysis and Acute Renal Failure in Babesiosis Requiring Erythrocytapheresis () - Nov 29, 2022 - Abstract #ASH2022ASH_6880;
He was appropriately fluid resuscitated and started on vancomycin and cefepime by emergency department...ID stepped down therapy to oral azithromycin 500mg daily and atovaquone 750 mg BID and doxycycline for total duration of two weeks at discharge.The indications of exchange transfusion in severe babesiosis are not well established...However we did not find any prospective randomized studies to support this approach. The data to support erythrocytapheresis versus whole blood or plasma exchange is limited at best in immunocompetent patients2,3,4,5 .Though it should be noted that a prompt reduction in parasitemia markedly improves mortality in immunocompromised patient such as ours especially when used adjunctively to antimicrobial therapy
- |||||||||| doxycycline / Generic mfg.
Journal: The Sall2 transcription factor promotes cell migration regulating focal adhesion turnover and integrin β1 expression. (Pubmed Central) - Nov 29, 2022
Accordingly, restoring Sall2 expression in the Sall2 iMEFs by using a doxycycline-inducible Tet-On system recovered cell migratory capabilities and focal adhesion dynamics...Altogether, our data showed that Sall2 promotes cell migration by modulating focal adhesion dynamics, and this phenotype is associated with SALL2/Sall2-transcriptional regulation of integrin β1 expression and FAK autophosphorylation. Since deregulation of cell migration promotes congenital abnormalities, tumor formation, and spread to other tissues, our findings suggest that the SALL2/Sall2-integrin β1 axis could be relevant for those processes.
- |||||||||| Asclera (polidocanol) / Merz Pharma
Journal: Systemic polidocanol from intravenous or pressurized intrauterine administration produces reversible cardiovascular toxicity. (Pubmed Central) - Nov 29, 2022
We continuously monitored blood pressure (BP) and heart rate using an arterial line during IV and intraperitoneal (IP) administration of polidocanol solution (PS) and polidocanol doxycycline solution in female rats and TC and IP administration of polidocanol foam (PF) and PDF (TC only) in female baboons...Pretreatment with diphenhydramine, ranitidine, or both did not block the refractory hypotension induced by IV administration of 1% PS (100 mg)...Adverse cardiovascular effects of polidocanol treatment occur owing to a direct myocardial effect of polidocanol and not as a result of a hypersensitivity reaction. Pressurized TC administration of PF results in refractory hypotension owing to endometrial vascular uptake of polidocanol and not as a result of uptake from peritoneal surfaces.
- |||||||||| doxycycline / Generic mfg.
Journal: Small and mighty - microRNAs pulling the strings. (Pubmed Central) - Nov 29, 2022
Furthermore the association between dysmobility syndrome and vertebral fractures is assessed, the role of doxycycline in schistosomiasis is elucidated, and the effect of stress on the blood-brain barrier is examined...Risk factors for recurrence of papillary thyroid cancer are identified, the outcome of reirradiation in oral squamous cell carcinoma is investigated, and the post-surgery outcomes in cases of infantile hypertrophic pyloric stenosis are reviewed. Finally this issue contains two articles about COVID-19, one describes the potential neurological damage left after the infection, and the second article analysis the outcome of uptake in vaccination against SARS-CoV-2.
- |||||||||| doxycycline / Generic mfg.
Modeling STXBP1 Loss of Function Using Human Pluripotent Stem Cell Derived 2D and 3D Models (Hall B, Level 3) - Nov 29, 2022 - Abstract #AES2022AES_1433;
Our results suggest that STXBP1 LoF disrupts cortical development and impairs spontaneous neurotransmitter release from presynaptic terminals. Future experiments will involve the forced expression of STXBP1 wildtype, pathogenic, benign and VUS in STXBP1 KO hPSCs to assay for rescue of the observed LoF phenotypes.
- |||||||||| doxycycline / Generic mfg.
A Neuronal Crispri Screen Identifies Novel Brain-specific Mtor Regulators () - Nov 29, 2022 - Abstract #AES2022AES_600;
Gene otology analysis of the average rank order across the three experiments identified “negative regulation of TOR signaling,” “TSC1-TSC2 complex”, and “GATOR1 complex” as the most enriched terms (Table 1). We next performed candidate screening of top candidate genes and known FCD genes (130 gRNAs total).
- |||||||||| doxycycline / Generic mfg., doxorubicin hydrochloride / Generic mfg.
P2 data, Journal: Combating Drug Resistance by Exploiting miRNA-200c-Controlled Phase II Detoxification. (Pubmed Central) - Nov 27, 2022
A combined treatment of these tumors with hsa-miR-200c and doxorubicin resulted in complete regression of the tumor in 60% of the animals. These results identify hsa-miR-200c as an important player regulating the cellular phase II detoxification, thus sensitizing cancer cells not expressing this microRNA to chemotherapeutics and reversing drug resistance through suppression of GSTs.
- |||||||||| doxycycline / Generic mfg., tetracycline / Generic mfg.
Preclinical, Journal: Tendon-Specific Activation of Tenogenic Transcription Factors Enables Keeping Tenocytes' Identity In Vitro. (Pubmed Central) - Nov 27, 2022
Primary tenocytes derived from this mouse line consistently expressed green fluorescent protein reporter transcription factors in response to doxycycline...In combination with the expression of BFP, which reports on the tendon-specific collagen I, and mScarlet, which reports on the tendon-specific transcription factor Scleraxis (Scx), we observed the more extended maintenance of the tendon cell identity of in vitro cultured tendon cells and Achilles tendon explants. This means that the Scleraxis bHLH transcription factor (Scx), mohawk homeobox (Mkx), early growth response 1 (Egr1) and early growth response 2 (Egr2) contributed to the maintenance of tenocytes' identity in vitro, providing a new model for studying extracellular matrix alterations and identifying alternative biomaterials in vitro.
- |||||||||| doxycycline / Generic mfg.
Biomarker, Journal: MT1-MMP Expression Levels and Catalytic Functions Dictate LDL Receptor-Related Protein-1 Ligand Internalization Capacity in U87 Glioblastoma Cells. (Pubmed Central) - Nov 27, 2022
MMP pharmacological inhibitors such as Ilomastat, Doxycycline and Actinonin increased in vitro An2 internalization by up to 2.5 fold within a human grade IV-derived U87 glioblastoma cell model...Collectively, our data suggest that MT1-MMP expression status dictates An2-mediated internalization processes in part by regulating cell surface LRP-1 functions. Such evidence prompts preclinical evaluations of combined MMP inhibitors/An2-drug conjugate administration to potentially increase the treatment of high-MT1-MMP-expressing brain tumors.
- |||||||||| doxycycline / Generic mfg., azithromycin / Generic mfg., erythromycin / Generic mfg.
Journal: Antibacterial Potential Analysis of Novel α-Helix Peptides in the Chinese Wolf Spider Lycosa sinensis. (Pubmed Central) - Nov 27, 2022
Together, it suggested that the two peptides interact directly on bacterial cell membranes. Taken together, bioinformatic and functional analyses in the present work sheds light on the structure-function relationships of LS-AMPs, and facilitates the discovery and clinical application of novel AMPs.
- |||||||||| doxycycline / Generic mfg.
Preclinical, Journal: Longitudinal CMR assessment of cardiac global longitudinal strain and hemodynamic forces in a mouse model of heart failure. (Pubmed Central) - Nov 27, 2022
After the first scan, the db/db animals received a doxycycline intervention to accelerate progression of HFpEF...Our results indicated that hemodynamic forces parameters were the only functional outcome measure that showed distinct temporal differences between groups. As such, changes in hemodynamic forces reflect early alterations in cardiac function which can be of added value in (pre)clinical research on HFpEF.
- |||||||||| doxycycline / Generic mfg.
Journal: Isoforms of Human MARCH1 Differ in Ability to Restrict Influenza A Viruses Due to Differences in Their N Terminal Cytoplasmic Domain. (Pubmed Central) - Nov 25, 2022
Herein, we used a doxycycline-inducible overexpression system to demonstrate that MARCH1.1 reduces titres of influenza A virus (IAV) released from infected cells whereas MARCH1.2 does not...Together, these studies describe the differential ability of MARCH1 isoforms to restrict IAV infectivity for the first time. Moreover, as published immunological, virological and biochemical studies examining the ability of MARCH1 to target particular ligands generally use only one of the two isoforms, these findings have broader implications for our understanding of how MARCH1 isoforms might differ in their ability to modulate particular host and/or viral proteins.
- |||||||||| Journal: Advances in pharmacotherapy for rosacea: what is the current state of the art? (Pubmed Central) - Nov 22, 2022
Current pharmacological treatment includes topical agents, spanning from several conventional (azelaic acid, metronidazole, sodium sulfacetamide) to new ones (brimonidine, oxymetazoline, ivermectine, minocycline), and systemic agents (doxycycline 40 mg modified-release), all Food and Drug Administration approved...Future investigations should be addressed to early inflammatory phyma or ocular rosacea, which have actually been neglected. Finally, there is still an ongoing need for therapeutic interventions able to relieve symptoms and social burden, all factors that greatly contribute to improve rosacea quality of life.
- |||||||||| Biomarker, Retrospective data, Journal, Next-generation sequencing: Clinical Characteristics of Chlamydia psittaci Pneumonia Confirmed by Metagenomic Next-Generation Sequencing. (Pubmed Central) - Nov 22, 2022
Finally, there is still an ongoing need for therapeutic interventions able to relieve symptoms and social burden, all factors that greatly contribute to improve rosacea quality of life. The use of mNGS increases the probability of diagnosing Chlamydia psittaci pneumonia, and good prognosis can be achieved with timely use of appropriate antibiotics.
- |||||||||| doxycycline / Generic mfg.
Journal: Doxycycline Preferred for the Treatment of Chlamydia. (Pubmed Central) - Nov 22, 2022
The use of mNGS increases the probability of diagnosing Chlamydia psittaci pneumonia, and good prognosis can be achieved with timely use of appropriate antibiotics. No abstract available
- |||||||||| doxycycline / Generic mfg., tetracycline / Generic mfg.
Journal: Antibiotics Limit Adaptation of Drug-Resistant Staphylococcus aureus to Hypoxia. (Pubmed Central) - Nov 22, 2022
In contrast, evolutionary adaptation by S. aureus to hypoxic conditions, which evolved in the absence of antibiotics through mutations affecting gyrB, was impeded by antibiotic treatment. Together, these data suggest that the horizontal acquisition of a new resistance mechanism is merely a starting point for the emergence of high-level resistance under antibiotic selection but that antibiotic treatment constrains pathogen adaptation to other important environmental selective forces such as hypoxia, which in turn could limit the survival of these highly resistant but poorly adapted genotypes after antibiotic treatment is ended.
- |||||||||| Journal: An analytical framework on the leaching potential of veterinary pharmaceuticals: A case study for the Netherlands. (Pubmed Central) - Nov 22, 2022
For VPs Sulfadiazine and Flubendazole we identify a few regions that are potentially prone to leaching, with leached quantities higher than 1 μg/ha...For Dexamethasone, even though applied on the soil in much lower concentrations compared to other investigated VPs, spatially widespread leaching to groundwater is found, with leached quantities higher than 1 μg/ha...This study has shown that the leaching potential of VPs is not determined by one single parameter, but by a combination of parameters. This combination also depends on the compound investigated.
- |||||||||| doxycycline / Generic mfg., erythromycin / Generic mfg.
Journal: Occurrence, spatial distribution and ecological risks of antibiotics in soil in urban agglomeration. (Pubmed Central) - Nov 21, 2022
(3) Ecological risks assessment of antibiotics in soils demonstrated that erythromycin (ERY), sulfamethoxazole (SMX), and doxycycline (DOX) may introduce high risks to soil ecosystem health, and more attention should be paid to the areas with intensive human activities that had potential high risk to soil ecosystem health. This study suggests that scientific land and soil management should be considered to prevent soil antibiotic pollution and sustain soil security in urban agglomeration.
- |||||||||| ceftriaxone / Generic mfg., doxycycline / Generic mfg.
Journal: Early Lyme neuroborreliosis manifesting as brachial plexopathy and meningitis in Northwestern Ontario, Canada: A case report. (Pubmed Central) - Nov 19, 2022
Lyme neuroborreliosis (LNB) is 1 presentation and can present with early or late manifestations; clinicians should maintain a high index of suspicion and begin empiric treatment in individuals with a clinical syndrome consistent with LNB. Early LNB manifestations have onset within 6 months of infection and include cranial and peripheral neuropathy, radiculitis, and aseptic meningitis; late LNB encompasses a chronic encephalomyelitis.
- |||||||||| ciprofloxacin oral / Generic mfg., vancomycin / Generic mfg., doxycycline / Generic mfg.
Journal: Mycoplasma hominis and Ureaplasma urealyticum infections after knee arthroplasty: A case report. (Pubmed Central) - Nov 19, 2022
M hominis and U urealyticum are common pathogens of the urinary system infections but they are rare in osteoarticular infections. In cases of fever, swelling and heat pain around the surgical site, joint fluid, negative blood culture and being irresponsive to anti-bacterial agents against the cell wall, special bacteria-related infection should be highly suspected.
- |||||||||| doxycycline / Generic mfg., tetracycline / Generic mfg.
Preclinical, Journal: Acute systemic knockdown of Atg7 is lethal and causes pancreatic destruction in shRNA transgenic mice. (Pubmed Central) - Nov 19, 2022
In a similar Atg5 knockdown model we neither observed loss of pancreatic integrity nor diminished survival after DOX treatment, but identified histological changes consistent with steatohepatitis and hepatic fibrosis in the recovery period after termination of DOX. Regulable Atg7-shRNA mice are valuable tools that will enable further studies on the role of autophagy impairment at various disease stages and thereby help to evaluate the consequences of acute autophagy inhibition as a therapeutic concept.
- |||||||||| doxycycline / Generic mfg.
Journal: Level of constitutively expressed BMAL1 affects the robustness of circadian oscillations. (Pubmed Central) - Nov 17, 2022
We applied transient response analysis to the Bmal1 promoter activity in the presence of various doxycycline concentrations. Based on the obtained transfer functions, we suggest that, at least in our experimental system, BMAL1 is not directly involved in the oscillatory process, but modulates the oscillation robustness by regulating basal clock gene promoter activity.
- |||||||||| doxycycline / Generic mfg.
Journal: Enhancement of fatty acid degradation pathway promoted glucoamylase synthesis in Aspergillus niger. (Pubmed Central) - Nov 17, 2022
This study suggested for the first time that glucoamylase production was significantly improved in A. niger by overexpression of genes fadE and fadA involved in fatty acids degradation pathway. Harnessing the intracellular fatty acids could be a strategy to improve enzyme production in Aspergillus niger cell factory.
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