- |||||||||| doxycycline / Generic mfg.
Primary U2AF1S34F Mutated Hematopoietic Cells Are Sensitive to Nonsense-Mediated RNA Decay Disruption In Vivo (SDCC - Ballroom 20CD) - Nov 3, 2023 - Abstract #ASH2023ASH_942;
To allow direct comparison of isogenic cells with or without U2AF1 S34F expression, half the mice were treated with doxycycline to induce U2AF1 S34F expression, and half of the mice did not receive doxycycline...Validation of these findings using primary mouse AML cells is ongoing, including the analysis of key regulators and protein biomarkers of the UPR pathway. The vulnerability of primary hematopoietic cancer cells with spliceosome mutations to NMD inhibition suggests the possibility for therapeutic targeting of NMD to treat myeloid malignancies with aberrant splicing.
- |||||||||| tamoxifen / Generic mfg., doxycycline / Generic mfg.
SIRT5 Regulates Energy Metabolism and Survival in Acute Lymphoblastic Leukemia Cells (Marriott Marquis - Marriott Grand) - Nov 3, 2023 - Abstract #ASH2023ASH_939;
Next, to evaluate dependency of SIRT5 -KD on multiple genotypes and therapy-resistant ALL, we engineered 30 ALL cell lines to stably express doxycycline sh SIRT5 representing B and T cell origin, diverse genotypes, and including drug resistant lines...Finally, to determine whether Sirt5 disruption affects energy metabolism in normal hematopoietic stem cells (HSCs), we generated a tamoxifen-inducible conditional hematopoietic cell-specific Sirt5 knockout (KO) model...Collectively, our results suggest that SIRT5 plays a critical oncogenic role in the development and maintenance of a subset of ALL, providing a rationale for the development of a clinical SIRT5 inhibitor. Targeting master regulators rather than single pathways may circumvent plasticity-based resistance in ALL.
- |||||||||| doxycycline / Generic mfg.
Journal: NOTCH3 signalling controls human trophoblast stem cell expansion and differentiation. (Pubmed Central) - Oct 31, 2023
Doxycycline-inducible expression of dominant-negative MAML1 in TSC lines provoked cell fusion and upregulation of genes specific for multinucleated syncytiotrophoblasts, which are the differentiated hormone-producing cells of the placenta...Accordingly, inhibition of NOTCH3 signalling diminished growth of TB-ORGs whereas overexpression of NOTCH3-ICD in primary CTBs and TSCs showed opposite effects. In conclusion, the data suggest that canonical NOTCH3 signalling plays a key role in human placental development promoting self-renewal of CTB progenitors.
- |||||||||| doxycycline / Generic mfg.
Journal: An Atypical Calf Cyst treated with Doxycycline Sclerotherapy: A Case Report. (Pubmed Central) - Oct 30, 2023
We suspect that complete resolution may have been due to repeated aspiration and injection, essentially performing longitudinal cyst fenestration, with possible contribution from doxycycline injection. Further study of intra-cyst doxycycline injection with re-aspiration after 10 minutes for treatment of refractory cysts may be warranted.
- |||||||||| Journal: Antimicrobial Resistance of Actinobacillus pleuropneumoniae, Streptococcus suis, and Pasteurella multocida Isolated from Romanian Swine Farms. (Pubmed Central) - Oct 28, 2023
For S. suis, the highest rates of resistance were observed for streptomycin (98%), tetracycline (75%), oxytetracycline (72%), doxycycline (52%), and erythromycin (51%)...Bacteria isolates maintained high susceptibility against antimicrobial agents usually used against the mainly respiratory tract pathogens of swine. Resistance for streptomycin, tetracycline, oxytetracycline, and tilmicosin was high for all the tested pathogens.
- |||||||||| Journal: Antimicrobial and Adjuvant Potencies of Di-n-alkyl Substituted Diazalariat Ethers. (Pubmed Central) - Oct 27, 2023
It is shown that in some cases, the lariat ethers have complex antimicrobials with considerable selectivity. Based on these observations, including 1:1 complexation between lariat ethers and antimicrobials and the cytotoxicity of the MeI salts showing a separation index of 32-fold, they hold significant potential for further development.
- |||||||||| doxycycline / Generic mfg.
Preclinical, Journal: Anti oxidative/neuro-inflammation properties of Withania somnifera root extract on rotenone induced stress in rat brain. (Pubmed Central) - Oct 27, 2023
Based on the observations, EWSR can be considered as an excellent source for neuroprotection, due to its significant anti-oxidative, anti-inflammatory, anti-neurodegenerative and anti-microglial properties when administered individually and in combination with known anti-inflammatory compounds (Doxycycline and Ellagic acids). But, further research is required before replacing the known neuroprotective treatments with phytochemical treatments.
- |||||||||| ampicillin / Generic mfg., doxycycline / Generic mfg., amoxicillin / Generic mfg.
Journal: Circulation of Antibiotic-Resistant Escherichia coli in Wild and Domestic Waterfowl in Ukraine. (Pubmed Central) - Oct 26, 2023
Analysis of antibiotic sensitivity phenotypes showed that only four antibiotic-resistant phenotypes were detected among non-MDR bacteria, whereas among the MDR bacteria, two antibiotic-resistant phenotypes were detected in mallards and six in domestic waterfowl. The results of this study showed that wild waterfowl in Ukraine, which live in natural conditions and do not receive any antimicrobial drugs, are carriers of E. coli that are resistant to a number of antibiotics that are actively used in industrial poultry.
- |||||||||| doxycycline / Generic mfg., tetracycline / Generic mfg.
Journal: Drug-induced sudden dysphagia. (Pubmed Central) - Oct 26, 2023
Doxycycline is a bacteriostatic antibiotic of the tetracycline group widely indicated in the treatment of numerous infections (skin, soft tissues...). Given its acid nature, doxycycline is able to potentially generate mucous damage acting as a caustic; therefore, when the capsule of doxycycline (high concentrated substance) is taken inappropriately (with a small quantity of liquid or just before laying down) and remains for a longer time in contact with the esophagus, doxycycline may cause esophagitis-type lesions and ulcerations.
- |||||||||| doxycycline / Generic mfg.
Preclinical, Journal: TP53-PTEN-NF1 depletion in human brain organoids produces a glioma phenotype in vitro. (Pubmed Central) - Oct 26, 2023
Specifically, we engineered a doxycycline-inducible vector encoding shRNAs enabling depletion of the TP53, PTEN, and NF1 tumor suppressors in human cerebral organoids...Transcriptomic analysis revealed a proneural GBM subtype. This proof-of-concept study offers a valuable resource for directly investigating the emergence and progression of gliomas within the context of specific genetic alterations in normal cerebral organoids.
- |||||||||| rifampicin / Generic mfg., doxycycline / Generic mfg., amoxicillin / Generic mfg.
Journal: Phenotypic characteristics and immune response of Procypris merus following challenge with aquatic isolate of Klebsiella pneumoniae. (Pubmed Central) - Oct 26, 2023
did not change significantly in any of the tissues, which might be a kind of immune response of P. merus against K.?pneumoniae infection. This study provides an important theoretical basis for the in-depth exploration of the pathogenic mechanism of K.?pneumoniae in fish and the immune response that occurs after the disease is contracted in fish, as well as theoretical support for the development of effective preventive and therapeutic strategies against K.?pneumoniae-infected aquatic animals in the future.
- |||||||||| norfloxacin / Generic mfg., doxycycline / Generic mfg., tetracycline / Generic mfg.
Journal: The role of Aeromonas genotyping in virulence for Dicentrarchus labrax. (Pubmed Central) - Oct 26, 2023
The presence of virulence genes was associated with bacterial pathogenicity expressed in fish mortality rate. Virulence genes also drastically affect cortisol levels more than catalase and glutathione peroxidase levels.
- |||||||||| doxycycline / Generic mfg.
Journal: Enhanced and synergistic catalytic activation by photoexcitation driven S-scheme heterojunction hydrogel interface electric field. (Pubmed Central) - Oct 24, 2023
Meanwhile, the 3D polymer chain spatial structure of chitosan hydrogel is conducive to rapid PMS capture and electron transport in advanced oxidation process, reducing the use of transition metal activator and limiting the leaching of metal ions. There is reason to believe that the synergistic activation of PMS by S-scheme heterojunction regulated by photoexcitation will provide a new perspective for future material design and research on enhancing heterologous catalysis oxidation process.
- |||||||||| ivermectin oral / Generic mfg., doxycycline / Generic mfg.
Review, Journal: New paradigms in research on Dirofilaria immitis. (Pubmed Central) - Oct 22, 2023
Since the advent of ivermectin (along with melarsomine and doxycycline), heartworm has come to be viewed as a solved problem in veterinary medicine, diminishing investment into non-clinical research on Dirofilaria immitis...A 'genomics toolbox' for D. immitis could enable unprecedented insight into the negotiations between host and parasite that enable survival in a permissive host. The more we know about the pathogen and how it manipulates its host, the better able we will be to protect companion animals far into the future.
- |||||||||| doxycycline / Generic mfg.
Journal: SYNCOPE: A RARE PRESENTATION OF LYME DISEASE. (Pubmed Central) - Oct 22, 2023
Lyme disease should be on the differential for patients presenting with vague, flu-like symptoms in the summer months in endemic areas. Treatment of early Lyme disease with doxycycline can prevent progression to secondary Lyme, which can present as a true cardiac or neurologic emergency.
- |||||||||| doxycycline / Generic mfg.
Preclinical, Journal: FKBP25 regulates myoblast viability and migration and is differentially expressed in in vivo models of muscle adaptation. (Pubmed Central) - Oct 22, 2023
Finally, a series of in vivo models of muscle adaptation were assessed, where it was observed that FKBP25 protein expression was increased in hypertrophy and regeneration conditions (chronic mechanical overload and the mdx model of Duchenne muscular dystrophy) but decreased in an atrophy model (denervation). Overall, the findings of this study establish FKBP25 as a regulator of myoblast viability and migration, with possible implications for satellite cell proliferation and migration and muscle regeneration, and as a potential regulator of in vivo skeletal muscle adaptation.
- |||||||||| doxycycline / Generic mfg., azithromycin / Generic mfg., acetylcysteine solution / Generic mfg.
Review, Journal: Selenium and COVID-19: A spotlight on the clinical trials, inventive compositions, and patent literature. (Pubmed Central) - Oct 22, 2023
Accordingly, there is good scope for scientists to work on developing novel and inventive selenium-based compositions to fight against COVID-19. However, there is also a need to consider the narrow therapeutic window and chemical interaction of selenium before developing selenium-based compositions.
- |||||||||| doxycycline / Generic mfg.
Preclinical, Journal: Protocol for auxin-inducible depletion of the RNA-binding protein PTBP1 in mouse embryonic stem cells. (Pubmed Central) - Oct 20, 2023
We describe steps for establishing an ESC line expressing doxycycline-inducible auxin receptor protein OsTIR1 and tagging endogenous Ptbp1 alleles using CRISPR-Cas9 and homology-directed repair reagents...This protocol is adaptable for other protein targets. For complete details on the use and execution of this protocol, please refer to Iannone et
- |||||||||| doxycycline / Generic mfg.
Trial primary completion date: Doxycycline in Cutaneous Schwannoma (NF2) (clinicaltrials.gov) - Oct 19, 2023
P1/2, N=19, Recruiting,
Clinical and biological improvement is often dramatic under antibiotic treatment. Trial primary completion date: Sep 2023 --> Sep 2024
- |||||||||| doxycycline / Generic mfg.
Trial completion date, Trial primary completion date: A Study of Doxycycline to Treat Chlamydial Infection (clinicaltrials.gov) - Oct 16, 2023
P4, N=664, Not yet recruiting,
Trial primary completion date: Sep 2023 --> Sep 2024 Trial completion date: Jul 2025 --> Nov 2025 | Trial primary completion date: Jul 2025 --> Nov 2025
- |||||||||| doxycycline / Generic mfg.
Targeted suicide gene therapy for cancer () - Oct 15, 2023 - Abstract #ESGCT2023ESGCT_841;
Tet?On 3G systems for Doxycycline?inducible ldrB gene expression was used as control...Moreover, in vivo studies under the control of the survivin promoter induced a severe loss of proliferation in vivo without side effects in our animal model. Given our findings, the combination of the ldrB gene and Survivin promoter holds promising potential as a viable option for future therapies in breast and cervical cancer.
- |||||||||| doxycycline / Generic mfg.
Transactivator?free lentiviral vectors for inducible Advanced Therapy Medicinal Products: application to CAR?T cells () - Oct 15, 2023 - Abstract #ESGCT2023ESGCT_799;
To overcome this challenge, we previously developed Lent?On?Plus (LOP), the first all?in?one transactivator?free lentiviral vectors (LVs) capable of regulating transgene expression in human stem cells using doxycycline (Dox).First and foremost, we compared our transactivator?free system, LOP, with a leading competitor that requires a transactivator derived from Herpes Simplex Virus (VP?16) to induce expression, which has been shown to cause different types of cellular toxicities by sequestering transcription factors and altering cell physiology...Moreover, the LOPIs2 system effectively regulated transgene expression in vivo following oral administration of Dox and reversed to basal levels after Dox withdraw. As a proof?of?concept, we validated the LOPIs2 system by generating inducible CAR?T cells capable of selectively eliminating CD19+ cells in the presence of Dox.In summary, our findings present the first transactivator?free, all?in?one system capable of generating Dox?inducible ATMPs, offering a safer alternative to currently available TetOn?systems.
- |||||||||| doxycycline / Generic mfg.
Development of physiological and inducible TRUCKs targeting pancreatic solid tumours () - Oct 15, 2023 - Abstract #ESGCT2023ESGCT_778;
In this model, the inoculation of ?CD19?iTRUCK?IL?18 cells showed a robust antitumor response that was enhanced when combined with oral administration of doxycycline. These results show that the endogenous control of CAR expression through TCR?like promoters, together with the exogenous control of IL18 release, constitute a promising strategy to generate safe and efficient CAR?T cell products against solid tumours.
- |||||||||| doxycycline / Generic mfg.
iTRUCK19.18: novel doxycycline?inducible IL?18 engineered CAR?T cells for the treatment of aggressive lymphomas () - Oct 15, 2023 - Abstract #ESGCT2023ESGCT_761;
Interestingly, doxycycline administration reduced TOX expression in iTRUCK19.18 patient cells after serial tumor challenges, suggesting that IL?18 not only enhances killing activity but also protects CAR?T cells from differentiating into terminally exhausted phenotypes. To our knowledge, iTRUCK19.18 cells are the first CAR?T cells able to control IL?18 expression exogenously and may represent an alternative to the application of conventional CAR?T cells in patients with certain aggressive malignancies.
- |||||||||| doxycycline / Generic mfg., amphetamine / Generic mfg.
Regulating transgene expression is essential to avoid toxic effects of supraphysiological GDNF doses in gene therapy for Parkinson's disease () - Oct 15, 2023 - Abstract #ESGCT2023ESGCT_721;
However, although Phase I uncontrolled clinical trials based on intraputaminal GDNF protein delivery were encouraging, placebo?controlled trials have failed to demonstrate significant clinical benefits.Our hypothesis is that GDNF beneficial effects are dose dependent.We have previously described a doxycycline (Dox)?regulated AAV vector allowing to adjust GDNF dose at clinically acceptable Dox doses...Behavioral tests revealing motor impairments were amphetamine?induced rotations and drug?free distance run and rotations in an Open field...In contrast the GDNF 3X treatment did not result in aberrant sprouting.In conclusion the GDNF?3X treatment was therapeutic according to all parameters by restoring DA neurons number and size as well as striatal innervation whereas, the 20X treatment restored none of these parameters. The GDNF 20X Int group restored DA neurons numbers and striatal innervation but not mean cell size.Only the GDNF 3X group showed no undesirable effects (oxidative stress or aberrant sprouting).In future clinical trials, it will be important to control GDNF administration to avoid overdosage potentially reducing the clinical benefits due to toxicity.
- |||||||||| doxycycline / Generic mfg., colistin sulphate / Generic mfg.
Journal: Situational Analysis and Knowledge, Attitudes, and Practices of Antimicrobial Use and Resistance among Broiler Poultry Farmers in Nepal. (Pubmed Central) - Oct 14, 2023
Farmers aged 31-40 showed a stronger inclination compared to other age groups (OR = 4.2, p = 0.02), and those using antimicrobials for preventive purposes had a more favorable attitude compared to those using them for other purposes (OR = 5.9, p = 0.02). In light of these findings, this study recommends the implementation of effective regulatory measures for drug usage, along with awareness programs addressing AMU and AMR to address the issue of AMR in poultry production.
- |||||||||| clindamycin phosphate / Generic mfg., rifampicin / Generic mfg., doxycycline / Generic mfg.
Journal: Treatment of Hidradenitis Suppurativa Evaluation Study (THESEUS): a prospective cohort study. (Pubmed Central) - Oct 12, 2023
In light of these findings, this study recommends the implementation of effective regulatory measures for drug usage, along with awareness programs addressing AMU and AMR to address the issue of AMR in poultry production. THESEUS established laser treatment and deroofing for HS in the UK and demonstrated their popularity with patients and clinicians for future RCTs.
- |||||||||| Review, Journal, HEOR, Cost-effectiveness, Cost effectiveness: From Breakouts to Bargains: Strategies for Patient-Centered, Cost-effective Acne Care. (Pubmed Central) - Oct 11, 2023
Frequent laboratory monitoring for spironolactone and isotretinoin continues to be prevalent despite little evidence to support its clinical utility, and it is associated with a major cost burden to the patient and health care system. The reduction of laboratory monitoring is an opportunity to provide higher-value care.
- |||||||||| doxycycline / Generic mfg.
Journal: PLAAT1 expression triggers fragmentation of mitochondria in an enzyme activity-dependent manner. (Pubmed Central) - Oct 11, 2023
We prepared HEK293 cells expressing mouse PLAAT1 in a doxycycline-dependent manner and found that the overexpression of PLAAT1 resulted in the transformation of mitochondria from the original long rod shape to a round shape, as well as their fragmentation...In PLAAT1-expressing cells, the level of dynamin-related protein 1 responsible for mitochondrial fission was increased, whereas those of optic atrophy 1 and mitofusin 2, both of which are responsible for mitochondrial fusion, were reduced. These results suggest a novel role of PLAAT1 in the regulation of mitochondrial biogenesis.
- |||||||||| metformin / Generic mfg.
Trial completion, Trial completion date: Metformin for the Treatment of Hidradenitis Suppurativa (HS) (clinicaltrials.gov) - Oct 11, 2023
P3, N=62, Completed,
N=900 --> 624 | Trial completion date: Jun 2023 --> Sep 2024 | Trial primary completion date: Mar 2023 --> Jul 2024 Not yet recruiting --> Completed | Trial completion date: Jan 2023 --> Aug 2023
- |||||||||| doxycycline / Generic mfg.
Journal: Deep flanking sequence engineering for efficient promoter design using DeepSEED. (Pubmed Central) - Oct 9, 2023
DeepSEED has demonstrated success in improving the properties of Escherichia coli constitutive, IPTG-inducible, and mammalian cell doxycycline (Dox)-inducible promoters. Furthermore, our results show that DeepSEED captures the implicit features in flanking sequences, such as k-mer frequencies and DNA shape features, which are crucial for determining promoter properties.
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