- |||||||||| doxycycline / generics
Preclinical, Journal: LdrB Toxin with In Vitro and In Vivo Antitumor Activity as a Potential Tool for Cancer Gene Therapy. (Pubmed Central) - Jul 25, 2019
...For that, colorectal and breast cancer cells were transfected under the control of a TRE3G promoter inducible by doxycycline...Scanning electronic microscopy demonstrates that the LdrB toxin conserves its pore-forming ability in HCT-116 cells as in E. coli k12. Taken together, our results provide, for the first time, a proof of concept of the antitumor capacity of the ldrB gene in colorectal and breast cancer.
- |||||||||| doxycycline / generics
Review, Journal: Doxycycline for Alzheimer's Disease: Fighting β-Amyloid Oligomers and Neuroinflammation. (Pubmed Central) - Jul 25, 2019
Doxy emerged as a promising preventive strategy in prion diseases and gave compelling pre-clinical results in mouse models of AD against Aβ oligomers and neuroinflammation. This strongly supports its therapeutic potential and calls for deciphering its exact mechanisms of action so as to maximize its effects in the clinic.
- |||||||||| ciprofloxacin / generics, doxycycline / generics, amoxicillin / generics
Journal: Antibiotic Resistance Profiles of Salmonella Recovered From Finishing Pigs and Slaughter Facilities in Henan, China. (Pubmed Central) - Jul 25, 2019
We conclude that the presence of alarmingly high resistance, toward the critical antibiotics such as fluoroquinolones and beta-lactams, in large swine farms in China, should draw public attention. These results highlight the need for continued antibiotic stewardship programs for judicious use of critical antibiotics in animal health as well as for producing safe pork.
- |||||||||| doxycycline / generics
Preclinical, Journal: A specific nanobody prevents amyloidogenesis of D76N β2-microglobulin in vitro and modifies its tissue distribution in vivo. (Pubmed Central) - Jul 24, 2019
...Here we compare the potency of two previously described inhibitors of wild type β-microglobulin fibrillogenesis, doxycycline and single domain antibodies (nanobodies)...In β2-microglobulin knock out mice, the D76N β2-microglobulin/ Nb24 pre-formed complex, is cleared from the circulation at the same rate as the uncomplexed protein; however, the analysis of tissue distribution reveals that the interaction with the antibody reduces the concentration of the variant protein in the heart but does not modify the tissue distribution of wild type β2-microglobulin. These findings strongly support the potential therapeutic use of this antibody in the treatment of systemic amyloidosis.
- |||||||||| rifampicin / generics, doxycycline / generics, ceftazidime / generics
Journal: Evaluating New Compounds to Treat Burkholderia pseudomallei Infections. (Pubmed Central) - Jul 24, 2019
An additional two compounds currently used to treat melioidosis, doxycycline and levofloxacin, were also identified in the screen...Auranofin, MMV688179, and MMV688271 reduced the bacterial population to an average of 4.53 × 10% compared to ceftazidime, which corresponds to 25.1% survival. Overall, our data demonstrates that auranofin, MMV688197, and MMV688271 have the potential to become repurposed drugs for treating melioidosis infections and the first evidence that alternative therapeutics can reduce B. pseudomallei persistence.
- |||||||||| doxycycline / generics
Journal: A method of producing genetically manipulated mouse mammary gland. (Pubmed Central) - Jul 24, 2019
With this system, gene transduction in the mammary gland can be easily and quickly achieved, and gene expression can be controlled by Dox administration. This system for genetic manipulation could be useful for analyzing genes involved in breast cancer.
- |||||||||| doxycycline / Generic mfg., Pierre Fabre
Biomarker, Journal, Residual disease: Impact of obesity on breast cancer recurrence and minimal residual disease. (Pubmed Central) - Jul 24, 2019
Doxycycline-inducible primary mammary tumors were generated in intact MMTV-rtTA;TetO-HER2/neu (MTB/TAN) mice or orthotopic recipients fed a high-fat diet (HFD; 60% kcal from fat) or a control low-fat diet (LFD; 10% kcal from fat)...These studies provide a genetically engineered mouse model for study of the association of diet-induced obesity with breast cancer recurrence. They demonstrate that this model recapitulates physiological changes characteristic of obese patients, establish that the association between obesity and recurrence risk is causal in nature, and suggest that obesity is associated with the increased survival and persistence of residual tumor cells.
- |||||||||| doxycycline / generics
Journal: A Platform for Studying Neurodegeneration Mechanisms Using Genetically Encoded Biosensors. (Pubmed Central) - Jul 23, 2019
For this purpose, we have created a series of plasmid constructs for monitoring endoplasmic reticulum stress, oxidative stress, apoptosis, and Ca2+-dependent hyperexcitability and generated transgenic iPSC line carrying mutation in the superoxide dismutase 1 gene (SOD1) and healthy control cell line. Both cell lines have specific transactivator sequence required for doxycycline-controlled transcriptional activation and can be used for a single-step biosensor insertion.
- |||||||||| doxycycline / generics
Review, Journal: Advances in Antiwolbachial Drug Discovery for Treatment of Parasitic Filarial Worm Infections. (Pubmed Central) - Jul 21, 2019
A concomitant effort was put towards developing validation approaches and in vivo models supporting drug discovery efforts. In this review, we summarize the strategies behind and outcomes of recent large phenotypic screens published within the last 5 years, hit compound validation approaches and promising candidates with profiles superior to doxycycline, including ones positioned to advance into clinical trials for treatment of filarial worm infections.
- |||||||||| doxycycline / generics
Clinical, Journal: Imported onchocerciasis in migrants and travelers. (Pubmed Central) - Jul 21, 2019
Although novel diagnostics have improved specificity, most remain restricted to tropical disease reference laboratories and to date there is no marker of cure. Prolonged doxycycline treatment may reduce the need for serial ivermectin, though more potent short-course macrofilaricidal drugs are being developed.
- |||||||||| doxycycline / generics
Clinical, Journal: Variation of the adulticide protocol for the treatment of canine heartworm infection: Can it be shorter? (Pubmed Central) - Jul 20, 2019
...Treatment began on day 0, with doxycycline for 30 days (10 mg/kg BID) and monthly ivermectin (6mcg/kg)...In addition, there is evidence that melarsomine is effective against worms under four months and macrocyclic lactones have some efficacy against heartworms older than two months. This modification allows a faster elimination of heartworms and a better compliance from the owners of the infected dogs.
- |||||||||| doxycycline / generics
Journal: Enhanced Intradermal Delivery of Nanosuspensions of Antifilariasis Drugs Using Dissolving Microneedles: A Proof of Concept Study. (Pubmed Central) - Jul 20, 2019
The dermatokinetic study revealed that the delivery of drugs into the dermis of excised neonatal porcine skin by MNs was significantly higher than that from a needle-free patch, with 29.29 ± 4.65%, 31.54 ± 5.35%, and 34.54 ± 4.98% of doxycycline, albendazole sulfoxide, and ivermectin retained in the dermis after 24 h. The results presented here serve as proof of concept for the significant enhancement of drug retention times in the dermis, following their formulation into NS and delivery via MN. Leading on from these studies, future work must investigate in vivo lymphatic pharmacokinetic profiling of drugs formulated into NS, in a suitable animal model.
- |||||||||| doxycycline / generics
Preclinical, Journal: Lessons from Cre-Mice and Indicator Mice. (Pubmed Central) - Jul 19, 2019
Based on these systems, it is evident that our knowledge of mature adipocyte categorization into brown, white, brite, or beige adipocytes is strongly influenced by the use of the various genetic mouse models described in this chapter. Our evaluation of different studies using the aforementioned systems focuses on key genes, which have been reported to maintain adipocyte's function (insulin receptor, Raptor, or Atgl).
- |||||||||| doxycycline / generics
Journal: An online field-assisted micro-solid-phase extraction device coupled with high-performance liquid chromatography for the direct analysis of solid samples. (Pubmed Central) - Jul 19, 2019
...The detection limits of nine PAHs including fluorene, phenanthrene, anthracene, fluoranthene, benzo[k]fluoranthene, benz[a]anthracene, benzo[b]fluoranthene, pyrene, and benzo[a]pyrene were all within 0.075-0.30 μg/kg, as well as four TCAs including oxytetracycline, tetracycline, chlortetracycline, and doxycycline were within 0.02-0.06 μg/kg...Beyond trace analytes analysis in solid and semi-solid matrixes, application of this total online analysis method can also be extended to investigate field-assisted extraction mechanisms. Graphical abstract.
- |||||||||| metformin / generics, doxycycline / generics
Clinical, Journal: A New Method for Ethical and Efficient Evidence Generation for Off-Label Medication Use in Oncology (A Case Study in Glioblastoma). (Pubmed Central) - Jul 19, 2019
P3
An example of an off-label drug protocol brought to the clinic for glioblastoma patients is presented, along with preliminary retrospective data from the METRICS study (NCT02201381). METRICS is a novel participant-funded, open-label, non-randomized, single-arm real-world study designed to gather high-quality evidence on the safety, tolerability, and effectiveness of four off-label metabolically targeted medicines as an adjunctive cancer treatment for glioblastoma patients.
- |||||||||| ciprofloxacin / generics, doxycycline / generics
Journal: The Australian Mid-West Coastal Marine Wound Infections Study. (Pubmed Central) - Jul 19, 2019
aureus or Vibrio species were frequently seen in marine wounds, and infections were often polymicrobial. Our results suggest that flucloxacillin (or clindamycin) and doxycycline (or ciprofloxacin) would be a reasonable combination for empirical oral therapy in adults.
- |||||||||| doxycycline / generics
Journal: Endoscopic Treatment of Ileal Pouch Sinus. (Pubmed Central) - Jul 19, 2019
NKSi-associated complications were reported in 6 (1.8% per procedure) cases. NKSi is an effective and safe procedure for treating pouch sinus.
- |||||||||| doxycycline / generics
Preclinical, Journal: Tau reduction in the presence of amyloid-β prevents tau pathology and neuronal death in vivo. (Pubmed Central) - Jul 19, 2019
...By suppressing human tau expression for 6 months in the APP/PS1 × rTg4510 mice using doxycycline, AT8 tau pathology, bioactivity, and astrogliosis were reduced, though importantly to a lesser extent than lowering tau in the rTg4510 alone mice...Nonetheless, lowering the expression of the human tau transgene was sufficient to equally ameliorate thioflavin-S positive tangles and prevent neuronal loss equally well in both the APP/PS1 × rTg4510 mice and the rTg4510 cohort. Together, these results suggest that, although amyloid-β stabilizes tau aggregates, lowering total tau levels is still an effective strategy for the treatment of tau pathology and neuronal loss even in the presence of amyloid-β deposition.
- |||||||||| doxycycline / generics
Journal: Characterization of Pasteurella multocida strains isolated from human infections. (Pubmed Central) - Jul 19, 2019
Each strain was susceptible to ampicillin, streptomycin, gentamycin, tetracycline, doxycycline, cefazolin, cefpodoxime, chloramphenicol, florfenicol and enrofloxacin. Common characteristics (virulence profile and antibiotic sensitivity pattern) shared by strains isolated from humans and cats support the view that domestic cats may serve as a potential reservoir for P. multocida.
- |||||||||| doxycycline / generics
Clinical, Review, Journal: Efficacy of Azithromycin in Treatment of Acne Vulgaris: A Mini Review. (Pubmed Central) - Jul 18, 2019
Common characteristics (virulence profile and antibiotic sensitivity pattern) shared by strains isolated from humans and cats support the view that domestic cats may serve as a potential reservoir for P. multocida. Available experimental data suggest that oral azithromycin is an effective and well-tolerated option for treatment of acne vulgaris.
- |||||||||| doxycycline / generics
Review, Journal: Review of Dancing Parasites in Lymphatic Filariasis. (Pubmed Central) - Jul 18, 2019
Therapeutic options include (combinations of) ivermectin, albendazole, diethylcarbamazine and doxycycline. The latter depletes endosymbiotic Wolbachia bacteria from the worms and thus sterilizes and later kills the adult worms (macrofilaricidal or adulticidal effect).
- |||||||||| doxycycline / generics
Journal: Doxycycline as Potential Anti-Cancer Agent. (Pubmed Central) - Jul 17, 2019
In this article, we try to introduce new a concept of cancer pharmacology, by offering new off-label use of Doxycycline, that characterized by selective toxicity, as potential anticancer agents. This notion is relying on the absence of taxonomic barriers.
- |||||||||| doxycycline / generics
Journal: Regulatory effects of Interleukin (IL)-15 on allergen-induced airway obstruction. (Pubmed Central) - Jul 17, 2019
Thirty percent of the endodontic isolates and 53% of the nosocomial isolates were equipped with blaTEM genes.The results suggest that the oral cavity can harbor E. faecalis strains with multiple resistances against different antibiotics and thus be regarded as a potential source of resistance traits. We report our novel finding that IL-15 has a potent inhibitory effect on the airway obstruction that occurs in response to environmental allergens.
- |||||||||| doxycycline / generics, doxorubicin hydrochloride / generics
Journal: Dysregulated mitogen-activated protein kinase signalling as an oncogenic basis for clear cell sarcoma of the kidney. (Pubmed Central) - Jul 17, 2019
The oncogenic mechanisms and tumour biology underpinning clear cell sarcoma of the kidney (CCSK), the second commonest paediatric renal malignancy, are poorly understood and currently, therapy depends heavily on doxorubicin with cardiotoxic side-effects...Since no primary CCSK cell line exists, we generated various stably transfected cell lines containing doxycycline-inducible HA-tagged YWHAE-NUTM2, in order to study the effect of expressing this transcript...Importantly, CCSK tumour samples similarly show upregulation/ activation of these pathways. These results support the oncogenic role of 14-3-3ε-NUTM2 in CCSK and provide avenues for the exploration of novel therapeutic approaches.
- |||||||||| Desoxyn (methamphetamine) / Lundbeck
Journal: Methamphetamine augment HIV-1 Tat mediated memory deficits by altering the expression of synaptic proteins and neurotrophic factors. (Pubmed Central) - Jul 17, 2019
Expression levels of Calmodulin kinase II (CaMKII), a marker of synaptodendritic integrity, were also significantly decreased in HIV-1 Tat mice that were treated with METH. Together, this data suggests that METH enhances HIV-1 Tat-induced memory deficits by reducing the expression of pre- and postsynaptic proteins and neuroplasticity markers, thus providing novel insights into the molecular mechanisms behind neurocognitive impairments in HIV-infected amphetamine users.
- |||||||||| doxycycline / generics
Journal: Immune-evasive gene switch enables regulated delivery of chondroitinase after spinal cord injury. (Pubmed Central) - Jul 17, 2019
...Therefore, here we apply a novel immune-evasive dual vector system, in which the chondroitinase gene is under a doxycycline inducible regulatory switch, utilizing a chimeric transactivator designed to evade T cell recognition...Thus, our novel gene therapy system provides an experimental tool to study temporal effects of extracellular matrix digestion as well as an encouraging step towards generating a safer chondroitinase gene therapy strategy, longer term administration of which increases neuroplasticity and recovery of descending motor control. This preclinical study could have a significant impact for tetraplegic individuals, for whom recovery of hand function is an important determinant of independence, and supports the ongoing development of chondroitinase gene therapy towards clinical application for the treatment of spinal cord injury.
- |||||||||| doxycycline / generics
Journal: Attempting to Convert Primed Porcine Embryonic Stem Cells into a Naive State Through the Overexpression of Reprogramming Factors. (Pubmed Central) - Jul 13, 2019
...In this study, doxycycline (DOX)-inducible vectors carrying Oct4, sex-determining region Y-box 2 (Sox2), Nanog, Kruppel-like family 4 (Klf4), or Myc, which are known reprogramming factors, were transduced into pESCs...Given these results, additional methods are needed to support the completion of each reprogramming phase to succeed in the conversion of the pluripotent state of pESCs. This study improves our understanding of pluripotent networks and can be used to aid in the establishment of bona fide pig pluripotent stem cells.
- |||||||||| doxycycline / generics
Clinical, Journal: Case Report: A Case of Severe Cerebral Malaria Managed with Therapeutic Hypothermia and Other Modalities for Brain Edema. (Pubmed Central) - Jul 13, 2019
In this report, we present a case of CM with associated severe brain edema that was successfully managed using a unique combination of therapeutic hypothermia, hypertonic saline, mannitol, and hyperventilation along with the antimalarial drugs quinidine and doxycycline. Our use of hypothermia was based on its proven benefit for improving neurological outcomes in post-cardiac arrest patients and previous in vitro research, suggesting its potential inhibitory role on malaria growth.
- |||||||||| doxycycline / generics
Preclinical, Journal: Generation of Mouse-Induced Pluripotent Stem Cells by Lentiviral Transduction. (Pubmed Central) - Jul 12, 2019
These so-called induced pluripotent stem (iPS) cells can give rise to any cell type of the body and thus have tremendous potential for many applications in research and regenerative medicine. Herein, we describe (1) a protocol for the generation of iPS cells from mouse embryonic fibroblasts (MEFs) using a doxycycline (Dox)-inducible lentiviral transduction system; (2) the derivation of clonal iPS cell lines; and (3) the characterization of the pluripotent potential of iPS cell lines using alkaline phosphatase staining, flow cytometry, and the teratoma formation assays.
- |||||||||| doxycycline / generics
Journal: The great imitator revealed: syphilis. (Pubmed Central) - Jul 12, 2019
Available data indicate success in reducing acquisition of syphilis with doxycyclinepre- and postexposure prophylaxis. This article is based on a presentation by Jeffrey D. Klausner, MD, MPH, at the 2018 Clinical Conference at the National Ryan White Conference on HIV Care and Treatment in December 2018.
- |||||||||| rifampicin / generics, doxycycline / generics
Journal: Soil bacterial communities of Sahara and Gibson deserts: Physiological and taxonomical characteristics. (Pubmed Central) - Jul 12, 2019
Tolerance of the axenic bacterial cultures, isolated from arid desert ecotopes, to temperature, pH, salts (KCl, NaCl, MgSO, NaHCO), strong oxidizers (Mg(ClO)), and antibiotics (ampicillin, cephalexin, chloramphenicol, tetracycline, doxycycline, kanamycin, rifampicin) was studied. The bacterial isolates were characterized by polyextremotolerance and by the ability to maintain metabolic activity in vitro while influenced by a wide range of physicochemical and biotic factors.
- |||||||||| doxycycline / Generic mfg.
Phase classification, Enrollment change, Trial completion date, Trial primary completion date: Oral Doxycycline for the Prevention of Syphilis in Men Who Have Sex With Men (DaDHS) (clinicaltrials.gov) - Jul 12, 2019
P4, N=52, Not yet recruiting,
The bacterial isolates were characterized by polyextremotolerance and by the ability to maintain metabolic activity in vitro while influenced by a wide range of physicochemical and biotic factors. Phase classification: P1 --> P4 | N=288 --> 52 | Trial completion date: May 2020 --> Dec 2021 | Trial primary completion date: Mar 2020 --> Jun 2021
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