- |||||||||| chymotrypsin / Generic mfg.
Journal: Molecular Sieving with PEGylated Dendron-Protein Conjugates. (Pubmed Central) - Jul 27, 2023
These dendrons were appended to the surface of azide-decorated ?-chymotrypsin (?-CT) via strain-promoted azide-alkyne cycloaddition to yield a library of dendron-protein conjugates...Furthermore, the results indicate that for most of the conjugates the PEG length had a more pronounced impact on enzyme activity than the dendron generation. Overall, the highest sieving ratios were found for ?-CT-dendron conjugates decorated with G3-PEG, G4-PEG, and G5-PEG, with the latter two structures offering the best combination of sieving ratio and small molecule activity.
- |||||||||| chymotrypsin / Generic mfg.
Journal: CELA3B immunostaining is a highly specific marker for acinar cell carcinoma of the pancreas. (Pubmed Central) - Jul 2, 2023
Chymotrypsin-like elastase family member 3B (CELA3B, elastase-3B) is a pancreatic enzyme with digestive function in the intestine...These included 1.2% of 91 adenoid cystic carcinomas, 1.2% of 246 mucoepidermoid carcinomas and 0.8% of 127 acinic cell carcinomas of salivary glands. Our data show a good sensitivity (75%) and a high specificity (99.9%) of CELA3B immunohistochemistry for diagnosing acinar cell carcinoma of the pancreas.
- |||||||||| chymotrypsin / Generic mfg.
Biomarker, Journal, Next-generation sequencing: Next-Generation Sequencing for Venomics: Application of Multi-Enzymatic Limited Digestion for Inventorying the Snake Venom Arsenal. (Pubmed Central) - Jul 1, 2023
Each venom was reduced and alkylated before being submitted to two different protocols: the classical bottom-up proteomics strategy including a digestion step with trypsin only, or MELD, which combines the activities of trypsin, Glu-C and chymotrypsin with a limited digestion approach...In light of these results, MELD represents a credible methodology to be applied as the next generation of proteomics approaches dedicated to venomic analysis. It may open new perspectives for the sequencing and inventorying of the venom arsenal and should expand global knowledge about venom composition.
- |||||||||| Tectin (tetrodotoxin) / CK Life Sci, Miostat (carbachol) / Novartis
Journal: E. coli infection disrupts the epithelial barrier and activates intrinsic neurosecretory reflexes in the pig colon. (Pubmed Central) - Jun 19, 2023
Exogenous addition of carbachol, vasoactive intestinal peptide, forskolin, 5-HT, nicotine, and histamine produced epithelial Cl secretion...The basal electrogenic ion transport remained increased until day 9 post-challenge and was decreased by tetrodotoxin (10M), atropine (10M), hexamethonium (10M), and ondansetron (10M)...An increase in mast cells, stained with methylene blue, was observed in the mucosa and submucosa but not in the muscle layer of ETEC-infected animals on day 9 post-challenge. ETEC increased the response of intrinsic secretory reflexes and produced an impairment of the colonic barrier that was restored on day 9 post-challenge but did not modify neuromuscular function.
- |||||||||| chymotrypsin / Generic mfg.
Journal: Cascade-Activated AIEgen-Peptide Probe for Noninvasively Monitoring Chymotrypsin-like Activity of Proteasomes in Cancer Cells. (Pubmed Central) - Jun 14, 2023
Significant correlation was found between the fluorescence signals of TPE and the viabilities of Btz-treated cells in concentration ranges from 0 to 1 ?M, indicating that TPE-1p could be employed to predict the activity of ChT-L inhibitors. The design of the cascade-activated AIEgen-peptide probe provides a viable approach for noninvasively monitoring the ChT-L activity of proteasomes in living cells, which facilitates high-throughput screening of ChT-L inhibitors in cancer therapy.
- |||||||||| chymotrypsin / Generic mfg.
Journal: P2X7 receptor inhibition ameliorates ubiquitin-proteasome system dysfunction associated with Alzheimer's disease. (Pubmed Central) - Jun 11, 2023
The design of the cascade-activated AIEgen-peptide probe provides a viable approach for noninvasively monitoring the ChT-L activity of proteasomes in living cells, which facilitates high-throughput screening of ChT-L inhibitors in cancer therapy. Our work demonstrates the sustained and aberrant activation of P2X7R caused by Tau-induced neuroinflammation contributes to the UPS dysfunction and subsequent neuronal death associated with AD, especially in the hippocampus.
- |||||||||| chymotrypsin / Generic mfg.
Journal: An N-glycopeptide MS/MS data analysis workflow leveraging two complementary glycoproteomic software tools for more confident identification and assignments. (Pubmed Central) - Jun 5, 2023
By manually interpreting the individual MS spectra, we report here the common sources of errors in assignment, especially those introduced by the use of chymotrypsin...A representative site-specific N-glycosylation pattern could be constructed based on quantifying only the overlapping subset of N-glycopeptides identified at higher confidence. Applying the two complimentary glycoproteomic software in a concerted data analysis workflow, we found and confirmed that glycosylation at several sites of an unstable Omicron spike protein differed significantly from those of the stable trimeric product of the parental D614G variant.
- |||||||||| CM-AT / Curemark
Trial completion date, Trial primary completion date: An Open Label Study of CM-AT for the Treatment of Children With Autism (clinicaltrials.gov) - May 23, 2023
P3, N=405, Active, not recruiting,
Applying the two complimentary glycoproteomic software in a concerted data analysis workflow, we found and confirmed that glycosylation at several sites of an unstable Omicron spike protein differed significantly from those of the stable trimeric product of the parental D614G variant. Trial completion date: Jun 2023 --> Jan 2025 | Trial primary completion date: Jan 2023 --> Jan 2025
- |||||||||| chymotrypsin / Generic mfg.
Preclinical, Journal: Rational Design of Esterase-Insensitive Fluorogenic Probes for In Vivo Imaging. (Pubmed Central) - May 5, 2023
This strategy was further extended to design highly specific fluorogenic probes for other representative targets, sulfites, and chymotrypsin. The present study expands the bioanalytical toolboxes available and offers a promising platform to develop esterase-insensitive cleavable fluorogenic probes for in vivo biosensing and bioimaging for the early diagnosis of diseases.
- |||||||||| chymotrypsin / Generic mfg.
Journal: Quantum Dots Meet Enzymes: Hydrophobicity of Surface Ligands and Size Do Matter. (Pubmed Central) - Mar 24, 2023
In this work, we investigated the interactions between QDs with different surface ligands and different particle sizes and ?-chymotrypsin (ChT) from the thermodynamic and kinetic perspectives...This work highlights the importance of hydrophobic ligands and particle sizes of QDs, which should be considered as the primary influencing factors in the assessment of biosafety. Meanwhile, the results herein can also inspire the design of nano inhibitors.
- |||||||||| chymotrypsin / Generic mfg.
Journal: Amino Acid Substitutions at P1 Position Change the Inhibitory Activity and Specificity of Protease Inhibitors BmSPI38 and BmSPI39 from Bombyx mori. (Pubmed Central) - Mar 16, 2023
The replacement of P1 residues with Arg or Lys not only reduced the intrinsic activities of BmSPI38 and BmSPI39, but also resulted in the acquisition of stronger trypsin inhibitory activities and weaker chymotrypsin inhibitory activities...In conclusion, this study not only confirmed that BmSPI38 and BmSPI39 had strong elastase inhibitory activity, but also confirmed that P1 residue replacement could change their activity and inhibitory specificity. This not only provides a new perspective and idea for the exploitation and utilization of BmSPI38 and BmSPI39 in biomedicine and pest control, but also provides a basis or reference for the activity and specificity modification of TIL-type protease inhibitors.
- |||||||||| chymotrypsin / Generic mfg.
Journal: How Sugars Protect Dry Protein Structure. (Pubmed Central) - Mar 9, 2023
We employed liquid-observed vapor exchange nuclear magnetic resonance (LOVE NMR), differential scanning calorimetry (DSC), and thermal gravimetric analysis (TGA) to show how trehalose and other sugars protect two model proteins: the B1 domain of streptococcal protein G (GB1) and truncated barley chymotrypsin inhibitor 2 (CI2)...Combining LOVE NMR and TGA data shows that water retention is not important. Our data suggest that sugars protect protein structure as they dry by strengthening intraprotein H-bonds and water replacement and that trehalose is the stress-tolerance sugar of choice because of its covalent stability.
- |||||||||| chymotrypsin / Generic mfg.
Journal: The path of proteolysis by bovine chymotrypsin. (Pubmed Central) - Mar 9, 2023
This study gave unique and quantitative insight in peptide formation and degradation by chymotrypsin in the digestion of proteins. The approach used showed potential to explore the path of hydrolysis for other proteases with less defined specificity.
- |||||||||| chymotrypsin / Generic mfg.
Journal: Low particle concentrations of nanoplastics impair the gut health of medaka. (Pubmed Central) - Mar 6, 2023
The trypsin, lipase, and chymotrypsin activities were increased, but the amylase activity was decreased...Moreover, NP exposures resulted in a decrease of diversity and changed the intestinal microbiota composition. Our results provide new evidence that long-term NPs exposure impaired the health of fish at extremely low particle concentrations, suggesting the need for long-term toxicological studies resembling environmental particle concentrations when assessing the risk of NPs.
- |||||||||| chymotrypsin / Generic mfg.
Generation and characterization of the Psmb8 G201V mutation knock-in mice as a model for Nakajo-Nishimura syndrome (ePoster Stage 1; Poster Hall space (NS Building)) - Mar 4, 2023 - Abstract #ISID2023ISID_408;
Psmb8 G201V/G201V mice spleen showed the presence of immature b5i subunit and significantly reduced 20S chymotrypsin-like activity, while 26S activity was retained, resulting in no apparent ubiquitin accumulation...A defect in 20S with retained 26S proteasome activities was observed also in two Japanese patients harboring a de novo heterozygous p.Gly156Asp mutation in PSMB9, showing proteasome-associated autoinflammatory syndrome with immunodeficiency (PRAAS-ID) and in Psmb9 G156D/+ mice showing the decrease in CD4 and CD8 T cells, B cells, and dendritic cells. In this presentation, we discuss how proteasome disability is associated with autoinflammation and immunodeficiency.
- |||||||||| chymotrypsin / Generic mfg.
Journal: Discovery, synthesis and mechanism study of 2,3,5-substituted [1,2,4]-thiadiazoles as covalent inhibitors targeting 3C-Like protease of SARS-CoV-2. (Pubmed Central) - Feb 27, 2023
Interestingly, these compounds were also shown to inhibit PL with the same level of IC values, but had negligible effect on proteases such as chymotrypsin, cathepsin B, and cathepsin L. Subsequently, the antiviral abilities of these compounds were evaluated in cell-based assays, and compound 6g showed potent antiviral activity with an EC value of 7.249 ?M...Our study revealed that the 2,3,5-substituted [1,2,4]-thiadiazole group could covalently modify the catalytic cysteine in the binding pocket of 3CL as a potential warhead. Moreover, 6a was a known GPCR modulator, and our study is also a successful computational method-based drug-repurposing study.
- |||||||||| chymotrypsin / Generic mfg.
Solvent and temperature regulation of chymotrypsin inhibitor 2 conformers measured by ESI-IMS-MS (Indiana Ballroom F (Indianapolis Marriott Downtown)) - Feb 14, 2023 - Abstract #ACSSp2023ACS_SP_10361;
With methanol addition to the solvent, we also observe ?H and ?S decreases compared to water at low temperatures, supporting our previous explanation. Detailed comparison allows rare insights into how solvents and temperatures regulate the populations of many solution structures of CI-2.
- |||||||||| chymotrypsin / Generic mfg.
Journal: Stable Enzymatic Nanoparticles from Nucleases, Proteases, Lipase and Antioxidant Proteins with Substrate-Binding and Catalytic Properties. (Pubmed Central) - Feb 12, 2023
Our objective was the fabrication of NP from 7 biomedicine-relevant enzymes, including DNase I, RNase A, trypsin, chymotrypsin, catalase, horseradish peroxidase (HRP) and lipase, the analysis of their conformation stability and enzymatic activity as well as possible toxicity for eukaryotic cells...Among the studied bioactive ENP, only DNase NP were highly toxic for 3 cell lines with granulation in 1 day posttreatment, whereas other NP were less toxic (if any). Taken together, the enzymes in the stable ENP retained their catalytic activity and might be used for intracellular delivery.
- |||||||||| chymotrypsin / Generic mfg.
Journal: The functional role of Ire1 in regulating autophagy and proteasomal degradation under prolonged proteotoxic stress. (Pubmed Central) - Feb 9, 2023
The chymotrypsin-like proteasomal activity was also significantly enhanced in these cells at later time points of aggregation...The results suggest that deletion of Ire1 activates UPR-independent arms of the proteostasis network, especially under conditions of aggravated stress. Thus, inhibition of Ire1 may regulate UPR-independent cellular stress-response pathways under prolonged stress.
- |||||||||| chymotrypsin / Generic mfg.
Journal: Goat milk-derived short chain peptides: Peptide LPYV as species-specific characteristic and their versatility bioactivities by MOF@FeO@GO mesoporous magnetic-based peptidomics. (Pubmed Central) - Feb 5, 2023
Relative high expression of chymotrypsin and pepsin by EnzymePredictor analysis could partially elaborate the reason of the abundance of SCPs in goat milk. Compared with bovine milk, further bioinformatics analysis indicated that goat milk could own higher nutritional value because of relative higher concentrations (>1 mg/L) of SCPs (LLV, FL, LVYP) with confirmed bioactivities including angiotensin-converting enzyme (ACE) inhibitor, antioxidant, dipeptidylpeptidase (DPP) III and DPP IV inhibitor, etc. Overall, this study opened a novel avenue for understanding versatility benefit of dairy products from a perspective of SCPs by using a developed MOF@FeO@GO mesoporous magnetic-based peptidomics.
- |||||||||| chymotrypsin / Generic mfg.
Journal: Nano-liquid chromatography with monolithic stationary phase based on naphthyl monomer for proteomics analysis. (Pubmed Central) - Jan 24, 2023
The potential of the final VNA column was demonstrated with a gradient elution in the separation of six intact proteins, including ribonuclease A (RNase A), cytochrome C (Cyt C), lysozyme (Lys), β-lactoglobulin (β-lac), myoglobin (My) and α-chymotrypsinogen (α-chym) in nano LC system. The column was then applied to the peptide analysis of trypsin digested cytochrome C, allowing a high peak capacity up to 1440 and the further proteomics analysis of COS-7 cell line was attempted applying the final monolithic column in nano-LC UV system.
- |||||||||| chymotrypsin / Generic mfg.
Retrospective data, Journal: A meta-analysis for assessing the contributions of trypsin and chymotrypsin as the two major endoproteases in protein hydrolysis in fish intestine. (Pubmed Central) - Jan 23, 2023
From the list of consulted articles, 21 studies were chosen based on correlation coefficients (Pearson correlation test), and four groups of fish were identified with high significant correlation between 1) the activity of chymotrypsin and total alkaline proteases; 2) the activity of trypsin, chymotrypsin, and total alkaline proteases; 3) the activity of trypsin and total alkaline proteases, and 4) mainly negative correlation between trypsin, chymotrypsin, and total alkaline proteases. These results indicated that the relative inputs of trypsin and chymotrypsin in protein hydrolysis may vary significantly among different fish species, which is a crucial point for proper understanding of species-specific digestive traits in both natural and aquaculture scenarios.
- |||||||||| chymotrypsin / Generic mfg., dexamethasone / Generic mfg., amiloride hydrochloride / Generic mfg.
Journal: Tocotrienols: Exciting Biological and Pharmacological Properties of Tocotrienols and other Naturally Occurring Compounds, Part I. (Pubmed Central) - Jan 11, 2023
Moreover, the effect of various tocotrienols (α-, γ-, or δ-tocotrienol), α-tocopherol, quercetin, riboflavin, (-) Corey lactone, amiloride, dexamethasone supplemented diets fed to chickens (4-weeks) resulted in reduction of total cholesterol, LDL-cholesterol, and triglycerides...In second study, hypercholesterolemic subjects were given increasing doses of δ-tocotrienol (125 mg, 250 mg, 500 mg, and 750 mg/day) plus AHA Step-1 diet (4-weeks). The most effective dose of tocotrienols (250 mg/day) may be used to lower serum NO (40%), CRP (40%), MDA (34%), γ-GT (22 %), and inflammatory cytokines IL-1α, IL-12, IFN-γ by 15% to 17%, and increase TAS levels by 22%.
- |||||||||| arecoline / Cogent
Review, Journal: Plant leads for mitigation of Oral Submucous Fibrosis: Current scenario and future prospect. (Pubmed Central) - Dec 25, 2022
Our results suggest that chymotrypsin C (CTRC) is degraded in an acidic environment, leading to the precipitation of lithostathine in the ductal lumen. We concluded that plant drugs like lycopene, curcumin, Aloe vera, colchicine, and Glycyrrhiza glabra, were found effective against OSMF in various in-vitro and/or clinical studies and are being used by modern and traditional practitioners.
- |||||||||| chymotrypsin / Generic mfg.
Journal: Binding and Unbinding Pathways of Peptide Substrates on the SARS-CoV-2 3CL Protease. (Pubmed Central) - Dec 21, 2022
At the early stage, the dominant motion was the diffusion on the protein surface showing a broad distribution, whose center was led into the cleft of the chymotrypsin fold. We observed a definite sequential formation of the hydrogen bonds at the later stage occurring in the cleft, initiated between Glu166 (3CL) and P3_Val (peptide), followed by binding to the oxyanion hole and completed by the sequence-specific recognition at P1_Gln.
- |||||||||| chymotrypsin / Generic mfg.
Journal: Replacing ZnSO with Zn-glycine in the diet of goat promotes the pancreatic function of the offspring. (Pubmed Central) - Dec 16, 2022
The activities of lipase and chymotrypsin were enhanced when replacing ZnSO with Zn-Gly...The results suggested that supply of dietary Zn-Gly to goats during late pregnancy promoted pancreatic function in offspring compared with dietary ZnSO supplementation. This provides new information about pancreatic function when supplementing different zinc sources in the diets of late pregnant goats.
- |||||||||| chymotrypsin / Generic mfg.
Journal: De Novo Design of a Highly Selective Nonpeptide Fluorogenic Probe for Chymotrypsin Activity Sensing in a Living System. (Pubmed Central) - Dec 16, 2022
The acquired probe, named TMBIHF, showed high selectivity toward various enzymes and reactive chemicals, while it retained high sensitivity and catalytic efficiency toward chymotrypsin. Moreover, TMBIHF was successfully applied for monitoring chymotrypsin activity and pancreas development in live zebrafish, specific sensing of exogenous and endogenous chymotrypsin in nude mice, and visualizing chymotrypsin-like activity-dependent cellular apoptosis, thus providing an alternative and reliable way for chymotrypsin-targeted biosensor or prodrug construction.
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