- |||||||||| morphine sulphate / Generic mfg.
Adolescent morphine exposure perturbs object recognition memory in female but not male rats (SDCC Halls B-H) - Oct 10, 2022 - Abstract #Neuroscience2022NEUROSCIENCE_8025;
In sum, our findings suggest female-specific sensitivity to morphine’s effects on recognition learning, whether the exposure was indirect, through parental use, or direct, through adolescent administration. This points to potential overlap between mechanisms underlying impacts of intergenerational and developmental morphine exposure.
- |||||||||| morphine sulphate / Generic mfg., duloxetine / Generic mfg.
Evaluation of behavioral pain phenotype in the rat monoiodoacetate and medial meniscal tear models of osteoarthritis pain (SDCC Halls B-H) - Oct 10, 2022 - Abstract #Neuroscience2022NEUROSCIENCE_7212;
The results from this study demonstrate that a variety of pain behaviors associated with knee joint osteoarthritis can be measured using the rat MIA model, while pain behaviors in the MMT model were less robust or not observable. Evaluation of novel assets following single and repeated administration in the MIA model using multiple pain endpoints may be a viable strategy to accelerate the development of non-opioid, non-addictive therapeutics for the treatment of osteoarthritis pain.
- |||||||||| morphine sulphate / Generic mfg.
Peripheral Expression of Pain Insensitivity Genes in Human Dorsal Root Ganglion Neurons and Satellite cells (SDCC Halls B-H) - Oct 10, 2022 - Abstract #Neuroscience2022NEUROSCIENCE_7181;
Additionally, we examined genes such as the mu-opioid receptor (OPRM1) and Transient Receptor Potential Vanilloid 1 (TRPV1), which are expressed in nociceptive neurons that are also responsive to morphine analgesic agents...The co-expression analysis with noci-responsive ion channels showed a high degee of overlap, but for some genes co-expression also was observed with molecular markers of non-nociceptive neurons and satellite cells. This latter observation is consistent with the range of clinical symptoms described for the affected individuals and involvement of non-painful neurons and support cells in the pathophysiology of neuropathy.
- |||||||||| morphine sulphate / Generic mfg.
Sex-specific analysis of opioid transporter expression at the blood-retina barrier (SDCC Halls B-H) - Oct 10, 2022 - Abstract #Neuroscience2022NEUROSCIENCE_7053;
We have recently shown that morphine accumulates in the mouse retina, but not hypothalamus, upon chronic systemic injection...Here, we expand on our work by using immunohistochemistry and quantitative reverse-transcription PCR to interrogate the expression of three opioid transporters (P-glycoprotein, breast cancer resistance protein and multidrug resistance protein 2) in the retina and hypothalamus. We compare transporter expression in males, females with a high estrogen/progesterone ratio (i.e. estrus/proestrus) and females with a low estrogen/progesterone ratio (i.e. metestrus/diestrus).
- |||||||||| morphine sulphate / Generic mfg.
Role of neuromedin s-expressing ventral tegmental area neurons in morphine behavior (SDCC Halls B-H) - Oct 10, 2022 - Abstract #Neuroscience2022NEUROSCIENCE_6164;
Future studies will determine whether VTA-NMS neuronal activity modulates morphine conditioned place preference. Our current data suggest that VTA-NMS neurons represent a subset of neurons that may be functionally relevant for morphine responses.
- |||||||||| morphine sulphate / Generic mfg.
A retinal contribution to opioid-induced sleep/wake dysregulation (SDCC Halls B-H) - Oct 10, 2022 - Abstract #Neuroscience2022NEUROSCIENCE_6163;
To confirm that morphine reaches the mouse retina following systemic delivery, we collected retina, hypothalamus and serum from adult male mice at different time points after 6 days of twice-daily 20 mg/kg morphine treatment...Taken together, these results support the idea that opioid that accumulation in the eye could persistently activate MORs on ipRGCs, altering the ability of ipRGCs to transmit light information to the brain’s sleep-wake circuitry. This alteration in photic input to the brain could underlie some of the sleep/wake problems associated with long-term opioid use.
- |||||||||| Olinvyk (oliceridine) / Trevena, Jiangsu NHWA Pharma
Assessment of pharmacological determinants behind oliceridine's therapeutic profile (SDCC Halls B-H) - Oct 10, 2022 - Abstract #Neuroscience2022NEUROSCIENCE_6162;
Using P-gp knock-out mice and rats, and procedures such as the hot plate, pulse oximetry, and intravenous self-administration, we determined that oliceridine’s in vivo potency as an analgesic and its adverse effect profiles (respiratory depression, abuse liability) are strongly determined by its P-gp activity. Finally, analysis of brain metabolic activity using positron emission tomography (PET) and [18F]fluorodeoxyglucose (FDG) in rats exposed to equipotent doses of morphine (10 mg/kg, SC) or oliceridine (1 mg/kg, SC) showed that each drug produced widespread decreases in brain metabolic activity.Together, these data suggest that oliceridine’s greater therapeutic index over other “non-biased” opioids such as morphine is determined to a large extent by its high potency and fast brain clearance due to P-gp.
- |||||||||| morphine sulphate / Generic mfg.
Biomimetic optogenetics: Reconstructing opiate-induced firing patterns reveals predicted reward (SDCC Halls B-H) - Oct 10, 2022 - Abstract #Neuroscience2022NEUROSCIENCE_6161;
Mathematical modelling of VTA GABA neuron activity patterns indicates that at baseline, firing can be described by a mixed Pareto-Log Normal model, whereas that Log Normal quality is lost once morphine is administered systemically. Our results suggest that firing patterns should be considered when using optogenetics for neurobehavioral research.
- |||||||||| morphine sulphate / Generic mfg.
Chronic Morphine Facilitates Opioid Signaling in a Behaviorally Relevant Thalamo-Striatal Circuit (SDCC Halls B-H) - Oct 10, 2022 - Abstract #Neuroscience2022NEUROSCIENCE_6160;
Moreover, this pathway may be an important driver of morphine-mediated hyperlocomotion, a behavioral effect to which sensitization develops. Understanding how chronic opioid exposure differentially alters opioid signaling based on brain region and subcellular locations, and how these effects relate to opioid-sensitive behaviors, will provide a better understanding of opioid function overall.
- |||||||||| morphine sulphate / Generic mfg.
Effects of Stress and Experimenter Related Experience on Brain Connectivity using fMRI and Network Analysis (SDCC Halls B-H) - Oct 10, 2022 - Abstract #Neuroscience2022NEUROSCIENCE_6152;
A theme of interest is the effects of pharmacological agents on the brain, particularly opioids such as morphine, and how it modifies in vivo functional brain circuits...Following the fMRI scan, animals will have blood withdrawn via the tail vein for markers of stress that will inform us of the effects of a single IP injection on brain connectivity fMRI markers. We will show that habituating rodents leads to a decrease in stress induced by the injection and more stable brain markers of connectivity derived from fMRI and a better understanding of the effects of opioids on brain connectivity.
- |||||||||| morphine sulphate / Generic mfg.
Sex differences in VTA GABA activity and affective behavior during withdrawal (SDCC Halls B-H) - Oct 10, 2022 - Abstract #Neuroscience2022NEUROSCIENCE_6147;
To model protracted withdrawal in rodents, mice underwent repeated morphine exposures followed by up to one week of abstinence...Underlying mechanisms of negative affect may differ by sex despite both males and females exhibiting some form of negative states during withdrawal. These results further our understanding of mesolimbic circuitry changes caused by opioids and highlight the need for nuanced behavioral modeling and inclusion of female subjects in future studies to balance the lopsided field.
- |||||||||| morphine sulphate / Generic mfg., methadone / Generic mfg.
Automated pupillometry for assessing withdrawal in patients with opioid use disorder (SDCC Halls B-H) - Oct 10, 2022 - Abstract #Neuroscience2022NEUROSCIENCE_6141;
Neither self-reported craving nor withdrawal was significantly associated with dose timing. These findings suggest that pupillometry measures are superior to commonly used self-report measures for assessing withdrawal in the context of MAT for OUD.
- |||||||||| morphine sulphate / Generic mfg.
Transcriptional and epigenetic mechanisms in the medial prefrontal cortex associated with individual differences in vulnerability to morphine self-administration in rats (SDCC Halls B-H) - Oct 10, 2022 - Abstract #Neuroscience2022NEUROSCIENCE_6131;
These epigenetic changes were significantly involved in gene networks associated with reward signaling and synaptic plasticity, including endocannabinoid, opioid, and dopamine feedback pathways, as well as synaptic long-term potentiation and depression. While our ATAC-seq data partially indicated long-term epigenetic regulatory changes associated with MSA vulnerability and gene dysregulation, further work on other epigenetic marks (e.g., histone modifications) with these models is planned to provide a more comprehensive understanding of mechanisms underlying opioid addiction vulnerability.
- |||||||||| morphine sulphate / Generic mfg.
The effect of early life stress on incubation of morphine craving (SDCC Halls B-H) - Oct 10, 2022 - Abstract #Neuroscience2022NEUROSCIENCE_6130;
These results suggest that while LBN reduces morphine taking, it does not affect cue-driven incubation of morphine craving. Understanding the precise mechanisms by which LBN affects addiction-related behavior will allow us to leverage this model to develop more targeted therapeutics.
- |||||||||| morphine sulphate / Generic mfg.
Reprogramming ZFP189 transcription factor function regulates drug specific behavioral responses and transcription (SDCC Halls B-H) - Oct 10, 2022 - Abstract #Neuroscience2022NEUROSCIENCE_6127;
To further examine the downstream relationship between ZFP189 and physiological response to saline, cocaine, and morphine, three reprogrammed synthetic ZFP189 variants were used to study behavioral responses...Combined with the CRISPR/dCas9 results, we propose that CREB’s activation of Zfp189 and ZFP189’s downstream function in NAc represents a cocaine-specific molecular cascade. Forward directions include examining the downstream targets of ZFP189 and its NAc cell-specific function.
- |||||||||| morphine sulphate / Generic mfg.
Evaluation of behavioral pain phenotype and optimization of the rat paclitaxel and oxaliplatin models of chemotherapy-induced peripheral neuropathy (SDCC Halls B-H) - Oct 10, 2022 - Abstract #Neuroscience2022NEUROSCIENCE_5642;
Initial pharmacological characterization was performed in these models by examining the effects of morphine sulfate (0.3-3 mg/kg, s.c.) on bilateral hind paw hypersensitivity, and morphine sulfate (3 mg/kg, s.c.) was found to significantly inhibit bilateral tactile and cold hypersensitivity in the paclitaxel and oxaliplatin models at Week 6 and Week 7, respectively. The validation of the paclitaxel and oxaliplatin models of chemotherapy-induced peripheral neuropathy further highlights efforts within the NIH HEAL Initiative’s PSPP program to validate clinically relevant endpoints and models to be incorporated into evaluating novel assets towards accelerating the development of novel non-opioid, non-addictive therapeutics.
- |||||||||| morphine sulphate / Generic mfg.
Mu-opioid receptors in astrocytes are important for morphine withdrawal (SDCC Halls B-H) - Oct 10, 2022 - Abstract #Neuroscience2022NEUROSCIENCE_5296;
These findings suggest that astrocyte MOR-1 could be involved in a homeostatic regulation of the endogenous opioid system via a functional link to oxidative phosphorylation in astrocyte mitochondria. We propose that astrocytes may provide new potential targets for ameliorating aversive states produced by opioid withdrawal.
- |||||||||| morphine sulphate / Generic mfg.
Oral ethanol self-administration in rats is modulated by intra-accumbal administration of M1 muscarinic acetylcholine receptors ligands (SDCC Halls B-H) - Oct 10, 2022 - Abstract #Neuroscience2022NEUROSCIENCE_4793;
For example, some studies have been reported that administration of a M1 acetylcholine receptor antagonist reduced rewarding properties of cocaine and morphine...The data showed that intra-accumbal injections of carbacol increased oral self-administration of EtOH, while pirenzepine reduced the oral self-administration of EtOH. These findings suggest that M1 muscarinic acetylcholine receptor ligands modulate the oral self-administration of EtOH in rats.This study was supported by PAPIIT IN301717 (UNAM, Mexico).
- |||||||||| morphine sulphate / Generic mfg.
Subchronic mitragynine causes sex-dependent changes in brain cytokine levels in rats with minimal cognitive and locomotor effects (SDCC Halls B-H) - Oct 10, 2022 - Abstract #Neuroscience2022NEUROSCIENCE_3545;
Preclinical psychopharmacology studies of mitragynine (MG), the main alkaloid of Kratom, indicate it has opioid-like effects, with lower potency than morphine...Interestingly, cytokine levels in the PFC were not impacted in male or female rats. In summary, despite nonsignificant effects in behavioral assessments of activity and spatial memory, brief intermittent MG exposure caused region- and sex-dependent changes in brain cytokine levels.
- |||||||||| morphine sulphate / Generic mfg.
Towards the development of brain-penetrating neurotensin receptor type 2 (nts2) non-opioid analgescis (SDCC Halls B-H) - Oct 10, 2022 - Abstract #Neuroscience2022NEUROSCIENCE_3101;
In parallel, we also monitor some physiological variables associated with NTS1 activation and determine whether this chimeric compound induces the adverse effects of morphine...Hence, the next step will be to conjugate An2 to our best NTS2-selective macrocycle. Together, this study opens the way to the development of safer non-opioid analgesics.
- |||||||||| morphine sulphate / Generic mfg., pregabalin / Generic mfg.
Comparison between Manual and Electronic von Frey for the Evaluation of Mechanical Allodynia in Rat Models of Neuropathic Pain. (SDCC Halls B-H) - Oct 10, 2022 - Abstract #Neuroscience2022NEUROSCIENCE_3084;
In this study, manual von Frey using the Chaplan method showed more sensitive measurement of the tactile allodynia as evidence by a better detection of pharmacological efficacy of the antiepileptic compound, the pregabalin. Our study evidenced a difference of sensitivity of both technics of evaluation of tactile and mechanical allodynia and help to be more relevant in the choice of the technic used depending on the pharmacological treatment with the objective of development of new antalgic compounds for the neuropathic pain treatment.
- |||||||||| morphine sulphate / Generic mfg.
An automated open-loop platform for the detection of pain and analgesia in mice (SDCC Halls B-H) - Oct 10, 2022 - Abstract #Neuroscience2022NEUROSCIENCE_3079;
Using this platform and trained deep learning networks, we then determined the efficacy of morphine analgesia by calculating the changes in behavior dynamics and clustering classifications across varying dosages of the opioid during acute inflammatory noxious events. We are now expanding this system’s capabilities to incorporate optogenetic control and calcium imaging of nociceptive and endogenous analgesia neural circuits in the periphery, spinal cord, and brain.
- |||||||||| morphine sulphate / Generic mfg., clozapine / Generic mfg.
Viral strategies for targeting and manipulating mu opioidergic neuronal populations for cell specific studies and therapeutic interventions (SDCC Halls B-H) - Oct 10, 2022 - Abstract #Neuroscience2022NEUROSCIENCE_3077;
In addition to mouse promoter driven constructs, we created a human OPRM1 promoter driven construct and confirmed its selective expression in OPRM1+ neurons in macaque PFC, insula, thalamus, and amygdala. Taken together, our new MORp viral toolkit provides researchers cell-type specific genetic access to putative MOR+ neurons in order to facilitate monitoring and manipulation of opioidergic neural circuits across a range of vertebrate species and translational screening models for pain and addiction.
- |||||||||| morphine sulphate / Generic mfg.
Identifying dopamine neuron subtypes recruited in opioid self-administration (SDCC Halls B-H) - Oct 10, 2022 - Abstract #Neuroscience2022NEUROSCIENCE_2577;
In light of the opioid crisis sweeping the nation, and the interaction between morphine and VTA neurons, we are investigating the activation pattern and projections of DA neurons following opioid administration...We present preliminary studies identifying VTA DA neuron populations involved in this functional activation. Such studies can aid in our understanding of distinct addiction circuitry and allow for improved medical intervention design.
- |||||||||| morphine sulphate / Generic mfg.
Characterization of Cocaine-induced Neural Activation and Gene Expression Change in Transcriptomic Cell Types of Mouse Mesocorticolimbic Areas (SDCC Halls B-H) - Oct 10, 2022 - Abstract #Neuroscience2022NEUROSCIENCE_2491;
Overall, we have successfully generated large-scale and high-quality scRNA-seq datasets to profile cocaine-induced neural activations and transcriptomic changes at a T-type level. Ongoing work focuses on the following directions: i) how much these cocaine-induced T-type activation patterns are similar to those from other drugs of abuse like morphine, ii) where these activated T-types are spatially located (by mapping it onto our whole-brain multiplexed error-robust fluorescence in situ hybridization (MERFISH) data), and iii) what other interesting DE genes are induced by chronic exposure to cocaine in non-neuronal T-types.
- |||||||||| morphine sulphate / Generic mfg.
The effects of perinatal morphine exposure on adolescent cognition and drug sensitization (SDCC Halls B-H) - Oct 10, 2022 - Abstract #Neuroscience2022NEUROSCIENCE_2235;
Overall, these data suggest that perinatal morphine exposure delays the formation of developmental milestones, protects against the effects of adolescent morphine exposure on recognition memory, and induces drug-specific effects on locomotor sensitization in adolescence. These findings highlight the nuanced effects of early-life opioid exposure and support further investigation into the long-term effects.
- |||||||||| morphine sulphate / Generic mfg.
Enduring Immune Consequences of Early-Life Opioid Exposure (SDCC Halls B-H) - Oct 10, 2022 - Abstract #Neuroscience2022NEUROSCIENCE_2234;
Preliminary data suggests that morphine exposed mice exhibit increased sickness behavior (as measured by reduced locomotor activity) 24 hrs following an LPS challenge when compared to saline controls (p < .05, n = 6 per group). Future work in the lab aims to further study the enduring inflammatory consequences—particularly in the neuroimmune system—of early life opioid exposure by molecularly profiling neuroinflammatory markers at multiple developmental timepoints (PNDs 14, 21, 60), thus developing a longitudinal profile of altered immune function in our mouse model of neonatal morphine exposure.
- |||||||||| morphine sulphate / Generic mfg.
Prefrontal Input to The Periaqueductal Gray Controls Placebo Analgesia (SDCC Halls B-H) - Oct 10, 2022 - Abstract #Neuroscience2022NEUROSCIENCE_1817;
We found that chemogenetic inactivation of a subpopulation of excitatory neurons in the ventrolateral periaqueductal gray (vlPAG) abolishes both morphine analgesia and morphine-conditioned placebo analgesia...For placebo to occur, mPFC-to-vlPAG neurons must be active during conditioning, consistent with a critical role for activity-dependent plasticity in this pathway. Our findings suggest that the vlPAG implements cognitive pain modulation by integrating top-down commands, at least some of which are conveyed directly from the cortex.
- |||||||||| morphine sulphate / Generic mfg.
Investigating the role of the locus coeruleus in periaqueductal gray-mediated antinociception (SDCC Halls B-H) - Oct 10, 2022 - Abstract #Neuroscience2022NEUROSCIENCE_1814;
Additionally, we find that vlPAG projection neurons send branching axons to both the RVM and LC, which may account for the LC activation we observed in both opioid-induced and PAG-driven antinociception. These findings reveal a previously underappreciated architecture of the descending pain modulatory circuitry and advance our understanding of the circuit mechanisms by which morphine produces analgesia.
- |||||||||| morphine sulphate / Generic mfg.
Anhedonia induced by HIV-1 Tat and morphine is associated by neuroinflammation and synaptodendritic injury in the prefrontal cortex (SDCC Halls B-H) - Oct 10, 2022 - Abstract #Neuroscience2022NEUROSCIENCE_1721;
Tat, but not morphine, decreased dendritic spine density on pyramidal neurons in layer V of the ACC and Tat increases hyperphosphorylated tau in the ACC and primary motor cortex. Together, our findings suggest that HIV-1 Tat and morphine differentially induce anhedonia and apathy and this depends on environmental factors and is associated with synaptic losses and neuroinflammation within the prefrontal cortex.
- |||||||||| morphine sulphate / Generic mfg.
HIV-1 Tat and morphine exposure dynamically shifts striatal monoamine levels and exploratory behaviors over time. (SDCC Halls B-H) - Oct 10, 2022 - Abstract #Neuroscience2022NEUROSCIENCE_1718;
Finally, Tat and morphine and duration of exposure interacted to affect locomotor activity, while having no effect on grip strength or rotarod performance. Together, these results provide novel insight into the unique HIV-1 Tat and morphine-induced interactive effects on dopamine and monoamine metabolism that in turn may drive dichotomous effects on neurocircuitry involved in motor and motivational behavior.
- |||||||||| morphine sulphate / Generic mfg.
Synaptic modulation of thalamocortical circuits by endogenous and exogenous opioids in mice (SDCC 24) - Oct 10, 2022 - Abstract #Neuroscience2022NEUROSCIENCE_536;
Endogenous opioids and exogenous opioid drugs like morphine can elicit pain relief through actions in the ACC...We injected CFA in the hind paw of naïve mice to induce chronic pain and five days later we euthanized these animals and examined synaptic transmission to determine how the responses to opioid drugs were affected by chronic pain. We hope that these results will contribute to the identification of novel pharmacological targets and the development of more selective opioid-based therapies for chronic pain management with lower risk of unwanted effects.
- |||||||||| morphine sulphate / Generic mfg.
Journal: The influence of preoperative anxiety on postoperative pain in patients undergoing cardiac surgery. (Pubmed Central) - Oct 9, 2022
For each extra year of age, the amount of morphine needed decreased by 0.26 mg, no difference was observed between men and women in terms of preoperative anxiety or postoperative analgesics requirements. It may be concluded that in cardiac surgery, postoperative analgesic requirements increased with higher levels of preoperative state anxiety and decreased for every extra year of age.
- |||||||||| Improving quality of life and symptom burden with opioids and adjuncts in lung cancer (IQSOL study) (Exhibition / Poster Area) - Oct 9, 2022 - Abstract #ESMOAsia2022ESMO_Asia_765;
G+A subgroup showed a 20.7% reduction of mean baseline morphine dose at subsequent visits...Antitussive properties of opioids appear to be accentuated when given along with adjuncts. The efficacy of adjuncts, though well studied in other chronic cough conditions, need to be evaluated further in thoracic malignancies, especially lung cancer.
- |||||||||| Prialt (ziconotide) / Jazz
Journal, HEOR: Ziconotide for the Management of Cancer Pain: A Budget Impact Analysis. (Pubmed Central) - Oct 8, 2022
The results of this budget impact analysis suggest that although a combination of intrathecal ziconotide in combination with morphine is associated with higher costs to the health care system in England, the incremental costs are not significant. Routine commissioning of ziconotide alone or in combination with morphine would provide an alternative for a population with limited ITDD treatment options.
- |||||||||| morphine sulphate / Generic mfg.
Retrospective data, Review, Journal: Quantifying unused opioids following emergency and ambulatory care: A systematic review and meta-analysis. (Pubmed Central) - Oct 8, 2022
Secondary outcomes were the proportion of patients with unused opioids following a prescription, the proportion of patients using no opioids, morphine equivalents of unused opioids, and factors associated with leftover opioids...More than 50% of opioids remain unused following prescriptions for acute pain. Responsible prescribing must be accompanied by education on safer use, storage, and disposal.
|
