- |||||||||| morphine sulphate / Generic mfg.
Neuronal Ensembles Regulating Opioid-Motivated Approach and Avoidance Behavior (MCP Hall A) - Aug 22, 2024 - Abstract #Neuroscience2024Neuroscience_7126;
To address this, we employed in situ hybridization (RNA scope) to determine whether there are phenotypic genetic differences between the two projecting pathways. Finally, we employed an opto-dialysis method to determine whether neurotransmitters differed among the morphine- vs. naloxone-reactive ensembles, suggesting unique neurotransmitter combinations are released to support the diametrically opposing behaviors.
- |||||||||| morphine sulphate / Generic mfg.
Neuropharmacology of cage-lid hanging behavior in mice: the role of endogenous opioid, cannabinoid, and corticosteroid systems. (MCP Hall A) - Aug 22, 2024 - Abstract #Neuroscience2024Neuroscience_6963;
To investigate if opioidergic modulation participates in hanging behavior, mice received morphine (1mg/Kg) or naloxone (3mg/Kg) IP 30 minutes before assessing hanging behavior...Animals under corticosterone treatment displayed a significant increase in immobility in the forced swim test on week 4, compatible with depression states, and a significant decrease in hanging (p<0.01; 2way ANOVA + Bonferroni). These findings suggest that hanging behaviour is modulated by opioidergic and endocannabinoid signaling, and its depression seems to be related to anhedonia.
- |||||||||| morphine sulphate / Generic mfg.
Social bonding and opioid modulation insights from prairie voles (MCP Hall A; Virtually Only) - Aug 22, 2024 - Abstract #Neuroscience2024Neuroscience_6897;
However, for voles cohabitating with females and forming a pair bond, morphine does induce conditioning. Consequently, opioids promote pair bond establishment and cohabitation with males but not with females, thus shielding voles from the addictive effects of opioids.
- |||||||||| morphine sulphate / Generic mfg.
Contribution of the prelimbic cortex to paraventricular thalamus pathway to heroin relapse (MCP Hall A) - Aug 22, 2024 - Abstract #Neuroscience2024Neuroscience_6893;
The prelimbic cortex (PL) is the major source of glutamatergic inputs to the PVT, and the PVT?PL pathway has recently been shown to be necessary for the retrieval of withdrawal memories in opioid-withdrawn mice following non-contingent morphine administration...To validate the functionality of the inhibitory hM4Di-DREADD, we performed whole-cell patch clamp recordings and found that bath application of J60 significantly reduced the excitability of PL neurons expressing hM4Di. Overall, our findings show that the PL?PVT pathway is necessary for cued relapse following abstinence from self-administered heroin and identify this pathway as a potential therapeutic target for OUD.
- |||||||||| morphine sulphate / Generic mfg.
Effect of repeated opioid dependence and withdrawal on operant social interaction (MCP Hall A) - Aug 22, 2024 - Abstract #Neuroscience2024Neuroscience_6892;
Overall, our findings show that the PL?PVT pathway is necessary for cued relapse following abstinence from self-administered heroin and identify this pathway as a potential therapeutic target for OUD. Pending an independent replication, results suggest that males and same-sex social interactions are more susceptible to the inhibitory effect of opioid dependence on social interaction.
- |||||||||| fentanyl citrate / Generic mfg.
Comparing The Nuclear Volume of Dark and Healthy Neurons in Fentanyl Exposed Mice (MCP Hall A) - Aug 22, 2024 - Abstract #Neuroscience2024Neuroscience_6877;
We present observations on a potential connection between altered nuclear morphology and Fentanyl exposure. In the literature the Dark Neuron story is complex, some reports question the validity of Dark Neurons as bona fide biological structures suggesting they are artifacts of tissue processing.
- |||||||||| morphine sulphate / Generic mfg., pramipexole IR / Generic mfg.
The dopamine D3 receptor agonist pramipexole decreases withdrawal in morphine tolerant rats (MCP Hall A) - Aug 22, 2024 - Abstract #Neuroscience2024Neuroscience_6866;
This provides preclinical evidence that the D3 receptor system may be an appropriate tool for developong a potential opioid replacement therapy. Future research will investigate if a stepwise decrease in doses of the morphine/PPX combination over time will also reduce withdrawal after complete removal of drug in morphine-tolerant animals.
- |||||||||| morphine sulphate / Generic mfg.
Early life resource scarcity and motivation for natural rewards in adolescent rats (MCP Hall A) - Aug 22, 2024 - Abstract #Neuroscience2024Neuroscience_6772;
We have previously used the limited bedding and nesting (LBN) model in rats to demonstrate that early resource scarcity has a sex-specific effect on reward-related behaviors in adults, such as self-administration of sucrose and social rewards, morphine self-administration, and delay discounting...Studies are ongoing but preliminary data suggest that, unlike in adulthood, LBN may not affect motivation for sucrose in adolescence. This suggests that maturation of reward circuits during the adolescent period may modulate the effects of early life scarcity and highlights the importance of studying the developmental trajectory of early stress on reward-related behavior.
- |||||||||| morphine sulphate / Generic mfg.
Thermal gradient test for in vivo assessment of pain in rodents (MCP Hall A) - Aug 22, 2024 - Abstract #Neuroscience2024Neuroscience_5969;
A noninvasive high-throughput test of quantifiable behaviors, as reported here, allows pain states and treatment efficacies to be evaluated with a higher degree of confidence. Removing researcher subjectivity allows for fast and reliable discovery of novel analgesics.
- |||||||||| morphine sulphate / Generic mfg.
Melanocortin receptor 4 agonist, setmelanotide, treats opioid-induced respiratory depression (MCP Hall A) - Aug 22, 2024 - Abstract #Neuroscience2024Neuroscience_5889;
C57BL/6J male mice with diet-induced obesity were treated with IP morphine (10 mg/kg) and then 15 min later with either SET (1 mg/kg IP) or vehicle VEH (IP) in a random order...In conclusion, SET effectively treated OIRD by increasing respiratory rate and inducing a significant decrease in the number of apneas without decreasing analgesia. Parafacial MC4R (+) neurons are a likely site of respiratory effects of SET.
- |||||||||| morphine sulphate / Generic mfg.
Exploring the link between negative emotional states and vulnerability to opioid misuse in a mouse model of neuropathic pain (MCP Hall A) - Aug 22, 2024 - Abstract #Neuroscience2024Neuroscience_4277;
By employing i.v. self-administration in mice, we studied the effects of SNIt in morphine self-administration, relapse-like behavior, and motivation to work for morphine infusions...Robust mechanical allodynia was observed in SNIt but not sham-operated or naive groups throughout the observation interval. Our results suggest that traumatic neuropathic nerve injury in the SNIt model did not reliably increase negative affective states or opioid vulnerability in male mice.
- |||||||||| morphine sulphate / Generic mfg., vigabatrin / Generic mfg.
Inhibition of GABA-aminotransferase (GABA-AT) with OV329 reverses pathological nociception in rodents without abuse liability (MCP Hall A) - Aug 22, 2024 - Abstract #Neuroscience2024Neuroscience_4269;
The next-generation GABA-AT inhibitor OV329, known for its superior efficiency in inactivating GABA-AT compared to vigabatrin, has shown promise as an anticonvulsant in preclinical studies...Behavioral assays included a conditioned place preference assay to assess reward or aversion induced by OV329 and an intravenous drug self-administration paradigm to determine its reinforcing properties compared to morphine...Overall, OV329 exhibited antinociceptive efficacy in preclinical models of CIPN and inflammatory pain, alongside a favorable safety profile at therapeutic doses. These findings suggest that targeting GABA-AT with OV329 may offer a novel analgesic mechanism with a more favorable therapeutic spectrum through enzyme regulation.
- |||||||||| morphine sulphate / Generic mfg.
Extensive alternative splicing in dorsal root ganglia sensory neurons is cell specific and selective to the regulation of neuronal ion channels and synaptic properties (MCP Hall A) - Aug 22, 2024 - Abstract #Neuroscience2024Neuroscience_4235;
Differentially spliced alternative exons between neuronal subtypes are enriched in genes involved in regulation of ion channel activity, morphine addiction, presynaptic membrane, and endocannabinoid signaling pathways (fold enrichment >10, p<0.001)...Our results demonstrate that extensive alternative splicing occurs in major subsets of sensory neurons affecting intrinsic neuronal and synaptic properties. The splicing of alternative exons in DRG neurons may arise from mechanistically different forms of transcript diversification likely initiated early during development.
- |||||||||| morphine sulphate / Generic mfg.
Investigating the role of focused ultrasound in modulating pain response by altering CGRP-mediated cascades in dorsal root ganglia (MCP Hall A) - Aug 22, 2024 - Abstract #Neuroscience2024Neuroscience_4075;
Opioids like morphine are recognized as potent analgesics; however, their narrow therapeutic window, the risk of addiction, and the development of tolerance significantly limit their applications...Additionally, the impact of FUS on capsaicin-triggered CGRP release was evaluated using primary DRG cells. Our results may open new avenues for developing therapeutic approaches to manage chronic pain conditions.
- |||||||||| morphine sulphate / Generic mfg.
Alterations in Anterior Cingulate Cortex Circuits by Inflammatory and Migraine-like Pain (MCP Hall A) - Aug 22, 2024 - Abstract #Neuroscience2024Neuroscience_3961;
These data suggests that CFA and morphine disrupt endogenous opioid signaling in the ACC in an opposing manner. Overall, these data suggest that PV cell function is altered in pain and drug-taking states in a treatment-dependent, sex- and synapse-specific manner which may contribute to divergent ACC output.
- |||||||||| morphine sulphate / Generic mfg.
A short peptide derived from the nature venom, acts as a non-opioid analgesic to attenuate mechanical hyperalgesia (MCP Hall A) - Aug 22, 2024 - Abstract #Neuroscience2024Neuroscience_3946;
This peptide, which we call P10581, is not toxic in mice/rats but shows a potent analgesic effect on mechanical hyperalgesia upon peripheral and central injections that can be as strong as morphine...Pharmacological inhibition of TRPV4 by P10581 in a heterogeneous expression system, combined with the use of TRPV4 knockout mice indicates that TRPV4 channels may act as the potential target for the analgesic effect of the peptide. This study identified a new potential therapeutic target for mechanical and neuropathic pain and introduced a natural toxin-based short peptide that produces potent analgesia via the blockade of the TRPV4 channel.
- |||||||||| morphine sulphate / Generic mfg., ketamine / Generic mfg.
Agmatine-based analog reduces tactile hyperalgesia through antagonism of NR2B-containing spinal NMDArs (MCP Hall A) - Aug 22, 2024 - Abstract #Neuroscience2024Neuroscience_3712;
The results of this study support the hypothesis that SSA is efficacious across multiple preclinical models of persistent pain, and that SSA shares a mechanism of action with agmatine. The improved biopharmaceutical features of SSA together with this subunit selectivity suggest that SSA may be a therapeutically useful non-opioid analgesic.
- |||||||||| morphine sulphate / Generic mfg.
Exploring the Role of GPR63 and GPR153 as Novel Modulators of Opioid-Induced Antinociception in a Mouse Model of Neuropathic Pain Via Glial Cell Modulation (MCP Hall A) - Aug 22, 2024 - Abstract #Neuroscience2024Neuroscience_3695;
This was achieved by in vivo transfection of targeted CRISPR-Cas9 DNA constructs with a universal promoter in the spinal cords of adult male and female CD-1 mice and evaluating changes in morphine response in a neuropathic pain model...This was done by replacing the universal promoter of our original CRISPR-Cas9 constructs with the Aif1 or Gfap promoters to restrict expression of the sgRNA to microglia or astroctyes, respectively. Together this work identifies completely novel pain modulators potentially acting through glial cells which could be exploited to develop new pain therapies.
- |||||||||| morphine sulphate / Generic mfg.
Design, in vitro and preclinical in vivo testing of the potent and safest hybrid opioid/neurotensin ligand for pain management (MCP Hall A) - Aug 22, 2024 - Abstract #Neuroscience2024Neuroscience_3692;
After central (i.t.) or systemic (i.p.) administration, this hybrid showed robust antinociceptive effects in acute and tonic inflammatory pain tests, outperforming at least a 10-fold dose of morphine...Whereas previous chimeric compounds led to a significant reduction in mean arterial pressure (40 mmHg), our lead compound was comparable to that of an intravenous bolus of saline. Altogether, this new bifunctional ligand represents a promising avenue towards the development of safer, more effective analgesics with reduced side-effect profiles.
- |||||||||| morphine sulphate / Generic mfg., sumatriptan succinate / Generic mfg.
Comparison of rat models of vascular headache and trigeminal sensitization in male and female rats (MCP Hall A) - Aug 22, 2024 - Abstract #Neuroscience2024Neuroscience_3650;
The data demonstrate that facial allodynia produced in rat models of vascular headache and trigeminal sensitization is generally more robust in female rats compared to male rats. Additionally, certain classes of analgesic compounds, including delta opioid agonists, are more effective in preventing the development of facial allodynia in male rats compared to female rats.
- |||||||||| morphine sulphate / Generic mfg.
Roles of EphB1 receptor in the development of opioid tolerance (MCP Hall A) - Aug 22, 2024 - Abstract #Neuroscience2024Neuroscience_3548;
Conditional deletion of EphB1 in the excitatory neurons in the dorsal root ganglion (DRG) and spinal dorsal horn (DH) neurons enhanced the analgesic effect of intrathecal morphine injection in mice under physiological and neuropathic/cancer pain conditions...EphB1 regulated GRK2-mediated phosphorylation of MOR at Ser375 and orchestrated MOR trafficking. Our findings strongly suggest that EphB1 receptor may serve as a driving force for the development of opioid tolerance through regulating and terminating MOR signaling, leading to the tolerance of opioid in antinociception.
- |||||||||| morphine sulphate / Generic mfg.
Analysis of Endomorphin Analogues as Candidate Drugs for Pain Relief (MCP Hall A) - Aug 22, 2024 - Abstract #Neuroscience2024Neuroscience_3545;
Our findings strongly suggest that EphB1 receptor may serve as a driving force for the development of opioid tolerance through regulating and terminating MOR signaling, leading to the tolerance of opioid in antinociception. Endomorphins are endogenous opioid peptides that have potential as analgesics and as a morphine substitute due to their selectivity and affinity for the
- |||||||||| morphine sulphate / Generic mfg.
Identifying brain regions involved in chronic-pain processing and analgesic treatment (MCP Hall A) - Aug 22, 2024 - Abstract #Neuroscience2024Neuroscience_3543;
Furthermore, we investigated the brain regions that were activated by the administration of a mu-opioid receptor agonist, morphine and a NOP receptor agonist, Ro 64-6198 in order to obtain a better understanding of the analgesic actions mediated by these opiates in the brain under a chronic pain condition...Our findings will allow us to understand the regulatory mechanism underlying pain-related behaviors and analgesic function produced by opiates by elucidating the brain regions in which cells are selectively activated during chronic pain or analgesic treatment. This knowledge may aid in the advancement of understanding where chronic pain and pain relief are happening in the brain and contribute to the development of targeted pain therapies to alleviate its debilitating effects.
- |||||||||| morphine sulphate / Generic mfg.
Spinal mechanisms underlying opioid-induced mechanical hypersensitivity and tolerance (MCP Hall A) - Aug 22, 2024 - Abstract #Neuroscience2024Neuroscience_3542;
Collectively, our data suggest that dorsal horn KORs could control morphine-induced mechanical forms of OIH and tolerance via modulating the excitability of dorsal horn KOR-expressing neurons. Targeting dorsal horn KORs/KOR-expressing neurons, could therefore provide preclinical studies and/or clinical trials a mechanism-based, modality-specific strategy to resolve opioid-induced mechanical forms of OIH and analgesic tolerance.
- |||||||||| morphine sulphate / Generic mfg., dimethyl fumarate / Generic mfg.
Dimethyl fumarate ameliorates deficits of cognition, mood, and opioid abuse in mice exposed to hiv-1-tat protein and/or morphine (MCP Hall A) - Aug 22, 2024 - Abstract #Neuroscience2024Neuroscience_2799;
As DMF is known to reduce ROS levels and increase BDNF levels and activate the master antioxidant transcription factor Nuclear Factor erythroid 2-related factor 2 (NFE2L2), leading to reductions in oxidative stress and improved mitochondrial function and biogenesis, brains were isolated from studied mice to assess these variables, with results to be discussed. Collectively, our findings may suggest new mechanistic insights into the dysfunction induced by exposure to HIV-Tat protein and opioids, while demonstrating that the DMF, currently clinically approved for treating multiple sclerosis, could benefit patients with HIV-1, OUD, or the comorbid disorder.
- |||||||||| morphine sulphate / Generic mfg.
Attenuation of Alzheimer (MCP Hall A) - Aug 22, 2024 - Abstract #Neuroscience2024Neuroscience_1572;
Here, we describe effects in older 5xFAD mice with higher beta-amyloid loads, whereby Hsp90? inhibition enhanced 24-hr spatial memory in MWM, ameliorated cognitive changes in OFT and EPM, improved nest-building, and restored thermal nociception via Hargreaves testing.
- |||||||||| morphine sulphate / Generic mfg.
Divergent opioid-mediated suppression of inhibition between hippocampus and neocortex across species and development (MCP Hall A) - Aug 22, 2024 - Abstract #Neuroscience2024Neuroscience_930;
Ongoing experiments seek to determine the effects of chronic opioid exposure on the developing hippocampus. Together, these findings demonstrate that PV-INs exhibit a divergence in opioid sensitivity across brain regions that is remarkably conserved across evolution and highlights the underappreciated role of opioids acting through immature PV-INs in shaping hippocampal development.
- |||||||||| morphine sulphate / Generic mfg.
Opioids potentiate medial habenula cholinergic afferent input onto interpeduncular nucleus GABAergic neurons (MCP Hall A) - Aug 22, 2024 - Abstract #Neuroscience2024Neuroscience_928;
Together, these findings demonstrate that PV-INs exhibit a divergence in opioid sensitivity across brain regions that is remarkably conserved across evolution and highlights the underappreciated role of opioids acting through immature PV-INs in shaping hippocampal development. We have employed a combined pharmacogenetic approach utilizing alternate agonists (e.g. Met-ENK, morphine), antagonists (e.g. CTAP) and OPRM1 KO mice to establish this
- |||||||||| morphine sulphate / Generic mfg.
Journal: Accidental epidural morphine overdose in a cat. (Pubmed Central) - Aug 22, 2024
We have employed a combined pharmacogenetic approach utilizing alternate agonists (e.g. Met-ENK, morphine), antagonists (e.g. CTAP) and OPRM1 KO mice to establish this No abstract available
- |||||||||| morphine sulphate / Generic mfg.
Journal, Surgery, Bariatric surgery: Implementation of updated enhanced recovery after bariatric surgery guidelines: adapted protocol in a single tertiary center. (Pubmed Central) - Aug 22, 2024
The addition of corticosteroids to intraoperative PAIs do not demonstrate a significant benefit in the majority of studies, and tend to not have an effect on PJI risk; however, studies were not specifically powered to assess PJI risk. Applied only in the study group, early ambulation (p?<?0.001), opioid restriction, and preventing postoperative nausea and vomiting (PONV), resulted in satisfactory scores (mean total visual analogue score 1.93?
- |||||||||| morphine sulphate / Generic mfg., loperamide / Generic mfg.
Journal: Phyto-pharmacological and computational profiling of Bombax ceiba Linn. Leaves revealed pharmacological properties against oxidation, hyperglycemia, pain, and diarrhea. (Pubmed Central) - Aug 22, 2024
Applied only in the study group, early ambulation (p?<?0.001), opioid restriction, and preventing postoperative nausea and vomiting (PONV), resulted in satisfactory scores (mean total visual analogue score 1.93? Additionally, both doses of the investigated extracts exhibited significant (p
- |||||||||| fentanyl citrate / Generic mfg., morphine sulphate / Generic mfg.
Journal: Mitigating Medication Tampering and Diversion via Real-time Intravenous Opioid Quantification. (Pubmed Central) - Aug 21, 2024
Finally, we demonstrate real-time fluidic measurements connected to a flow cell to simulate IV administration and a blind study classified using a machine-learning algorithm. The system achieves limits of detection (LODs) of 1.26 ?g/mL and 2.75 ?g/mL for fentanyl and morphine, respectively, while operating with >1-month battery lifetime due to an optimized ultra-low power 36 ?A sleep mode.
- |||||||||| fentanyl citrate / Generic mfg., morphine sulphate / Generic mfg., hydromorphone hydrochloride / Generic mfg.
Clinical, Journal, Surgery: Opioid Dose Variation in Cardiac Surgery: A Multicenter Study of Practice. (Pubmed Central) - Aug 21, 2024
Much of this variation is attributable to practice variability rather than patient or surgical differences. This suggests an opportunity to optimize opioid use in cardiac surgery.
- |||||||||| morphine sulphate / Generic mfg.
Journal: Drug dependence epidemiology in palliative care medicinal cannabis trials. (Pubmed Central) - Aug 20, 2024
This study identified alcohol and tobacco as the main substances used in this group of patients and that most were of very low risk for drug dependence. This suggests routine drug screening for palliative care patient may not be justified, but the high possibility of questionnaire bias is acknowledged.
- |||||||||| morphine sulphate / Generic mfg.
Post-Colonoscopy Intussusception: A Rare Phenomenon (Exhibit Hall E) - Aug 20, 2024 - Abstract #ACG2024ACG_4273;
She was started on IV fluids, morphine, broad spectrum antibiotics and surgery was consulted...As more cases are reported, we would be able to better define more risk factors and counsel patients appropriately. Figure: Abdominopelvic Computed tomography (CT) on presentation showing colo-colonic intussusception (white arrow) on axial (A), coronal (B) and sagittal (C) views as well as repeat CT the following day showing resolution-red arrow (D).
- |||||||||| hydroxychloroquine / Generic mfg.
Pill Swallowing: A Case of Misdirection - Hydroxychloroquine Induced Pill Esophagitis Complicated by an Aberrant Right Subclavian Artery (Exhibit Hall E) - Aug 20, 2024 - Abstract #ACG2024ACG_2823;
Patient was given lidocaine and morphine for symptomatic relief and discharged on steroids for pleurisy...She was initiated on pantoprazole...Although her ARSA was not the cause of her dysphagia/chest pain, clinicians should be aware that it can be a cause of these symptoms, although it may be infrequent. Figure: Aberrant Right Subclavian Artery (ARSA) which arises as a separate branch of the thoracic aorta, and courses behind the esophagus.
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