- |||||||||| morphine sulphate / Generic mfg.
Central metabolism as a potential origin of sex differences in morphine analgesia but not in the induction of analgesic tolerance in mice (Virtual Only) - Dec 20, 2021 - Abstract #Neuroscience2021Neuroscience_7249;
However, morphine metabolism does not seem to be involved in the induction of morphine analgesic tolerance. ACKNOWLEDGMENTS AND GRANT SPONSOR - This work was funded by CNRS, University of Strasbourg (Unistra), French Ministère Délégué à la Recherche et à l'Enseignement Supérieur and FHU Neurogenycs, French National Research Agency (ANR; contract ANR-17-EURE-0022, EURIDOL graduate school of pain).; Grant Support: ANR; contract ANR-17-EURE-0022, EURIDOL graduate school of pain
- |||||||||| morphine sulphate / Generic mfg.
Cell type characterization of the lateral septum during morphine dependence and withdrawal (Virtual Only) - Dec 20, 2021 - Abstract #Neuroscience2021Neuroscience_7233;
While Nts and Sst cells have been implicated in fear and avoidance behaviors, there is currently little known about their role in drug dependence and withdrawal. These data will provide an atlas for septal gene expression and supply insight into LS cell types that are involved in opioid dependence and affective behaviors.; Grant Support: UW ADAI; NIH R37 DA032750; NRSA MH117931
- |||||||||| morphine sulphate / Generic mfg.
Deep brain stimulation shortens extinction of persistent morphine-seeking. (Virtual Only) - Dec 20, 2021 - Abstract #Neuroscience2021Neuroscience_7015;
Reinstatement at 7 days after the final day of extinction showed a significant reduction in drug-seeking, in animals who received LF-DBS (29%; p < 0.005), as compared to 57% of animals in the sham-DBS group. Our data suggest a potential therapeutic effect of LF-DBS in refractory patients of addiction by reducing both the amount of time required to fully extinguish drug-seeking, and relapse.; Grant Support: NIH-RISE-R25GM061838; NIH-MBRS SCORE-1SC2DA047809-02
- |||||||||| morphine sulphate / Generic mfg.
Inhibition of PSD95-nNOS protein-protein interactions decreases morphine reward and relapse vulnerability in rats (Virtual Only) - Dec 20, 2021 - Abstract #Neuroscience2021Neuroscience_7011;
Our results provide behavioral and neurochemical support for the hypothesis that inhibition of PSD95-nNOS protein-protein interactions decreases morphine reward and relapse without producing abuse liability on its own. Our studies highlight a previously unrecognized role for the use of PSD95-nNOS disruptors as a novel non-narcotic therapeutic strategy that shows promise for treating opioid addiction.; Grant Support: NIH/NIDA DA042584 to AGH and GVR
- |||||||||| fentanyl citrate / Generic mfg.
Role of orexin-1 receptor signaling in demand for the opioid fentanyl in adult females (Virtual Only) - Dec 20, 2021 - Abstract #Neuroscience2021Neuroscience_6988;
Further analysis will be performed to elucidate the role of estrous cycle in fentanyl seeking female rats. In conclusion, our study supports OxR1 antagonist as a potential pharmacotherapy for opioid addiction in women.; Grant Support: National Institute On Alcohol Abuse And Alcoholism of the National Institutes of Health under Award Number T32AA028254
- |||||||||| fentanyl citrate / Generic mfg., morphine sulphate / Generic mfg.
Interleukin-1 receptor-associated kinase 4 (IRAK4) in the nucleus accumbens regulates opioid-seeking behavior (Virtual Only) - Dec 20, 2021 - Abstract #Neuroscience2021Neuroscience_6847;
In addition, inhibition of IRAK4 also reduced the cue-induced reinstatement of fentanyl-seeking. Our findings suggest an important role of IRAK4 in opioids relapse-like behaviors and provide novel evidence in the association between innate immunity and drug addiction.; Grant Support: NIDA Grant R21DA040777; NIDA Grant R01DA047967
- |||||||||| morphine sulphate / Generic mfg.
Relation among potential measures of morphine vulnerability in rats (Virtual Only) - Dec 20, 2021 - Abstract #Neuroscience2021Neuroscience_6845;
In addition, a lower short-term response to higher doses of morphine predicted a more intense acute withdrawal. Overall, results suggested that a behavioral measure of morphine's short-term inhibitory effect, such as a measure of arouse-ability, might predict morphine acute withdrawal and morphine vulnerability.
- |||||||||| morphine sulphate / Generic mfg.
Modulation of reward sensitivity by morphine administration and physiological state (Virtual Only) - Dec 20, 2021 - Abstract #Neuroscience2021Neuroscience_6843;
Lever pressing was significantly reduced during morphine administration and returned to baseline during withdrawal. Collectively, these results suggest that acute states of physiological need generally increase reward sensitivity while chronic need states generally reduce reward sensitivity despite specificity in food and fluid intake in response to need.; Grant Support: NIH Grant DA025634 (MFR)
- |||||||||| morphine sulphate / Generic mfg.
The effects of sex and ovarian hormones on morphine-induced anxiety and withdrawal behaviors (Virtual Only) - Dec 20, 2021 - Abstract #Neuroscience2021Neuroscience_6840;
Very preliminary evidence suggests that regardless of hormone treatment, morphine-treated female rats display more anxiety-related behavior compared to their matched saline-treated groups both 12 and 108 hours into withdrawal (a phenomenon not yet observed in male rats). The running of additional planned cohorts as well as the analysis of somatic withdrawal observation is currently underway.
- |||||||||| morphine sulphate / Generic mfg.
Bac-mediated overexpression of striatopallidal neuron specific gene gpr6 heightened aversive effects of opiate withdrawal (Virtual Only) - Dec 20, 2021 - Abstract #Neuroscience2021Neuroscience_6787;
We evaluated these animals using a conditioned place aversion (CPA) assay using the µ opioid receptor antagonist naloxone, either alone or after chronic morphine exposure...To decipher the possible transcriptomic mechanism through which Gpr6 modulates withdrawal aversion, we subjected BAC-Gpr6 mice and wildtype controls to low-dose naloxone and performed RNA sequencing in tissue from dorsolateral and ventral striatum, two regions enriched in µ opioid receptor. We will present our findings on genes that are differentially expressed based on Gpr6 gene dosage increase and hence could play a role in modifying aversive behaviors in opiate withdrawal.; Grant Support: NIH/NIDA P50DA005010
- |||||||||| Lucemyra (lofexidine) / US WorldMeds, Stada
Dual agonism at mu-opioid and adrenergic-alpha 2 receptors are necessary to produce the discriminative-stimulus effects of the primary kratom alkaloid mitragynine in rats (Virtual Only) - Dec 20, 2021 - Abstract #Neuroscience2021Neuroscience_6784;
The kratom alkaloid mitragynine may combine two critical pharmacological mechanisms in a single molecule, mu-opioid and adrenergic-alpha 2 agonism, that currently is achieved clinically with separate drugs (e.g., methadone and lofexidine, respectively, for opioid use disorder/withdrawal syndrome)...Using rats discriminating i.p. 32 mg/kg of mitragynine from its vehicle, here we compared time-effect functions (5, 15, 30, 60, 90, 120, 180, 240, 360, and 240 prior to sessions) of substitution of morphine, the Aα 2 R agonists (clonidine and lofexidine) and antagonist (yohimbine)...The dose-effect function of mitragynine discrimination was shifted rightward and downward by the opioid receptor antagonists naltrexone (0.032 mg/kg) and naloxone (0.1 mg/kg), as well as by the Aα 2 R antagonists yohimbine and atipamezole (both at 3.2 mg/kg). These results suggest that mitragynine exerts its in vivo effects via dual agonism at µ-opioid and adrenergic-α 2 receptor.; Grant Support: NIDA Grant R01 DA047855; NIDA Grant UH3 DA048353
- |||||||||| morphine sulphate / Generic mfg.
Morphine accumulates in the mouse retina following systemic injection (Virtual Only) - Dec 20, 2021 - Abstract #Neuroscience2021Neuroscience_6781;
Interestingly, results from this study not only show that systemically administered morphine is deposited in the retina, but also that morphine remains there long after the drug has been metabolized in the blood. This supports the idea that opioids could persistently activate MOR on ipRGCs, altering the ability of ipRGCs to transmit light information to the brain's sleep-wake circuitry.; Grant Support: NIH R01 EY029227
- |||||||||| morphine sulphate / Generic mfg.
Exercise tendencies modulate changes to opioid or exercise-induced reward (Virtual Only) - Dec 20, 2021 - Abstract #Neuroscience2021Neuroscience_6774;
Adult male Wistar rats from each of these groups were given a rewarding stimulus of either access to a running wheel or an injection of morphine...It was confirmed that running wheel access and opioid administration were comparably rewarding in all groups. The conclusion is that, compared to HVR and WT, LVR animals with their different genotypes and phenotype also have a unique pattern of behavioral responses to an opioid and to running.
- |||||||||| morphine sulphate / Generic mfg.
Taste Reactivity to Sucrose in Differentially Reared Rats Altered by Mu Opioid Receptor Functioning (Virtual Only) - Dec 20, 2021 - Abstract #Neuroscience2021Neuroscience_6768;
In the current experiment, we examine changes in hedonic responding for sucrose in differentially reared rats, while enhancing MOR function via morphine injections...These data indicate that activation of MORs may decrease 'liking' in isolated rats, and increase 'liking' in enriched rats. These results suggest that isolation rearing may alter MOR function to change incentive salience for reinforcers.; Grant Support: NIH P20GM113109
- |||||||||| morphine sulphate / Generic mfg.
Sex differences in distress-related behavior and reinstatement of opioid seeking in mice that witness a conspecific under predatory threat (Virtual Only) - Dec 20, 2021 - Abstract #Neuroscience2021Neuroscience_6764;
Here, we investigated whether male and female mice showed divergent responses following the observation of a conspecific under predatory threat.Male and female C57/Bl6 mice were trained in a conditioned place preference (CPP) paradigm, in which one chamber was paired with morphine (15mg/kg), and the opposite chamber with saline...In both sexes, administration of the oxytocin antagonist rescued observational distress-related behavior.These data reveal sex-related differences in responding to the distress of another. Our paradigm may offer a means of further investigating sex differences in the induction of psychosocial stress and in stress-induced reinstatement in mice.; Grant Support: Engdahl Family Foundation
- |||||||||| morphine sulphate / Generic mfg.
Goal-directed decision making is disrupted during morphine withdrawal (Virtual Only) - Dec 20, 2021 - Abstract #Neuroscience2021Neuroscience_6760;
Together, these data suggest that acute morphine withdrawal is associated with a deficit in goal-directed decision making. Ongoing experiments using sucrose licking tasks are assessing the influence of withdrawal and morphine-paired context on the motivational and hedonic processes that govern reward consumption.; Grant Support: DA050116; DA050116-02S1
- |||||||||| morphine sulphate / Generic mfg.
Amygdala-related behavioral phenotype of prenatal opioid exposure depends on history of early life stress (Virtual Only) - Dec 20, 2021 - Abstract #Neuroscience2021Neuroscience_6736;
C57/Bl6 dams were given access to 0.3 mg/ml morphine in drinking water sweetened with 0.2% saccharin (MO, n = 24) from 10 days prior to conception until offspring were weaned on postnatal day (P)21, and control dams were given 0.2% saccharin (SCH, n = 16)...Importantly, this replicates our prior studies demonstrating that prenatal MO exposure increases behavior related to exploration or novelty seeking, while prenatal MO combined with early life stress reduces exploration. This indicates that the behavioral effects of POE manifest differently if combined with early life stress.; Grant Support: NIH Grant K99 DA049908
- |||||||||| morphine sulphate / Generic mfg.
Repeated morphine exposure alters prelimbic cortex activity and induces risk-taking behavior in rats (Virtual Only) - Dec 20, 2021 - Abstract #Neuroscience2021Neuroscience_6622;
Taken together, our results demonstrate that opioid-induced CPP is associated with reduced inhibition of PL activity following repeated administrations of morphine. In addition, after repeated opioid exposure, PL neurons failed to respond to threat stimuli, and this pattern of neuronal activity occurred alongside increased risk-taking behavior during approach-avoidance conflict.; Grant Support: NIH R00-MH105549; Rising STARs Award from the University of Texas System; NIH-R01-MH120136; NARSAD Young Investigator
- |||||||||| morphine sulphate / Generic mfg.
Clinical features correlated with comethylation modules in mexican teenagers with eating disorders (Virtual Only) - Dec 20, 2021 - Abstract #Neuroscience2021Neuroscience_6597;
Black module was non-significant enriched in morphine addiction synaptic vesicle cycle, apoptosis, and mTOR signaling pathway. Meanwhile, yellow module was non-significant enriched in alpha-Linolenic acid metabolism, VEGF signaling pathway, and hepatitis C. These data suggest that perturbations in DNA methylation could correlate with somatometric measures and comorbidities in individuals with ED.
- |||||||||| morphine sulphate / Generic mfg.
Differential Effects of Mitragynine and Morphine on Unconditioned Food and Water Intake (Virtual Only) - Dec 20, 2021 - Abstract #Neuroscience2021Neuroscience_6289;
These data suggest that opioidergic signaling mediates the hyperphagic effects of mitragynine but hypophagia induced by the high dose of mitragynine might recruit other receptors populations. Naltrexone was ineffective reversing the adipsic effects of morphine and mitragynine, which suggests that a non-opioid mechanism is involved for fluid intake.Supported by National Institute on Drug Abuse grants UH3 DA048353 and R01 DA047855.; Grant Support: UG3 DA048353; R01 DA25267
- |||||||||| morphine sulphate / Generic mfg.
A translational rodent model of gestational buprenorphine or morphine exposure: offspring outcomes (Virtual Only) - Dec 20, 2021 - Abstract #Neuroscience2021Neuroscience_6036;
Offspring brains collected at postnatal day 2 will be analyzed to determine the effects of gestational opioid exposure on brain-derived neurotrophic factor (BDNF) concentration in the hippocampus and prefrontal cortex as a measure of neurodevelopment. More research is needed to understand how buprenorphine affects pregcy outcomes and subsequent offspring well-being in order to avoid negative consequences in human mothers undergoing opioid maintece treatment.; Grant Support: Betty Neitzel Research Award; ReBUILDetroit Bridge Award
- |||||||||| morphine sulphate / Generic mfg.
Coordinated neural dynamics of breathing across the medulla and pons (Virtual Only) - Dec 20, 2021 - Abstract #Neuroscience2021Neuroscience_5693;
In addition, we record from these distributed neural populations during administration of opiates (morphine) and observe that the respiratory network as a whole slows, but does not drastically reconfigure, in response to opiates. Together, these data provide an integrated view of the dynamic coordination of neural activity driving breathing in the intact animal.; Grant Support: R01 HL144801; R01 HL151389; R01 HL126523
- |||||||||| morphine sulphate / Generic mfg., baclofen / Generic mfg.
Inhibition of Heat shock protein 90 in the Spinal Cord Enhances Opioid Anti-Nociception by Disabling an Inhibitory GABA Circuit (Virtual Only) - Dec 20, 2021 - Abstract #Neuroscience2021Neuroscience_4874;
These results taken together suggest that IT 17-AAG potentiates morphine-induced antinociception via upregulation of GAT-2, relieving an inhibitory GABAergic brake on opioid antinociception. This study provides mechanistic insight into how Hsp90 inhibitors can be used to boost opioid antinociception and may have identified a novel inhibitory circuit of opioid antinociception.; Grant Support: Institutional funds from the University of Arizona; Arizona Biomedical Research Commission New Investigator Award #ADHS18-198875
- |||||||||| morphine sulphate / Generic mfg.
Heat shock protein 90 inhibition in spinal cord enhances opioid anti- nociception by suppressing EGFR and AMPK signaling (Virtual Only) - Dec 20, 2021 - Abstract #Neuroscience2021Neuroscience_4873;
When 17-AAG, an Hsp90 inhibitor, is administered to the spinal cord (intrathecally, IT), there is an augmentation in morphine induced antinociception in mice...This suggests that these molecules act as negative feedback breaks on opioid anti-nociception in the spinal cord which leads to the enhanced opioid antinociception. This can be reversed when either an EGFR or AMPK agonist is administered, thus elucidating key molecular mechanisms to establish the basic science foundation by which Hsp90 is regulating opioid signaling.; Grant Support: Arizona Biomedical Research Commission New Investigator Award
- |||||||||| morphine sulphate / Generic mfg.
Advanced age and sex modulate multiple mu opioid receptor signaling mechanisms in the rat midbrain periaqueductal gray: Implications for analgesia (Virtual Only) - Dec 20, 2021 - Abstract #Neuroscience2021Neuroscience_4872;
We are currently assessing age and sex differences in vlPAG MOR phosphorylation using immunoprecipitation of MOR phosphorylated at serine 375 and assessing opioid-induced cAMP inhibition using TR-FRET cAMP immunoassay to elucidate the mechanisms whereby morphine potency is reduced in aged animals. These novel findings of age-induced attenuation in MOR signaling within the PAG provide potential therapeutic targets to improve pain management in the elderly.; Grant Support: NIH R01DA041529
- |||||||||| morphine sulphate / Generic mfg., pramipexole IR / Generic mfg.
Preference for morphine is attenuated in the presence of the dopamine 3 receptor agonist pramipexole (Virtual Only) - Dec 20, 2021 - Abstract #Neuroscience2021Neuroscience_4871;
In contrast, 33% of rats (both male and female) increased consumption from the bottle of morphine alone. Animals exposed to the morphine/PPX combination displayed no changes in locomotor activity, while animals exposed to morphine alone showed increased locomotor activity at 1 week (males) or 4 weeks (females).In conjunction with previous studies showing that PPX enhances the analgesic effects of low dose morphine, these results suggest that the morphine/PPX drug combination represents a potential pain therapy that can be used long-term without producing addictive behaviors.; Grant Support: East Carolina University Human Behavior Research Core
- |||||||||| morphine sulphate / Generic mfg.
Treatment with a novel Aha1 inhibitor, KU177, improves opioid therapy (Virtual Only) - Dec 20, 2021 - Abstract #Neuroscience2021Neuroscience_4870;
Our previous studies demonstrated that treatment with Hsp90 inhibitors in the spinal cord enhanced the morphine antinociception and reduced the tolerance in different pain models...Furthermore, KU-177 dose-dependently increased the Nrf2 activation in the BV-2 cells with or without lipopolysaccharides (LPS) stimulation. These findings demonstrate that Aha1 inhibitor could be a potential approach for improving opioid therapy for pain management.; Grant Support: SC INBRE 21-4275
- |||||||||| fentanyl citrate / Generic mfg., methadone / Generic mfg.
Truncating intracellular C-termini encoded by exon 7 of mu opioid receptor gene, Oprm1, in C57BL/6J mice attenuates fentanyl and methadone tolerance and reward (Virtual Only) - Dec 20, 2021 - Abstract #Neuroscience2021Neuroscience_4868;
Fentanyl and methadone-induced β-arrestin2 recruitment in mMOR-1O, a full length E7-associated 7TM variant, was investigated in Be(2)C cells using with a Nanobit-based β-arrestin2 recruitment assay. Together, these data further support the functional relevance of E7-associated splice variants in opioid pharmacology.; Grant Support: NIH NIDA grant DA007242; The Peter F. McManus Trust; NIH NIDA grant DA042888; NIH CA08748; The Mayday Foundation; Rutgers Brain Health Institute; Rutgers New Jersey Medical School
- |||||||||| Vumerity (diroximel fumarate) / Biogen
Opioid-sparing effects of the Nrf2 activator diroximel fumarate in mice (Virtual Only) - Dec 20, 2021 - Abstract #Neuroscience2021Neuroscience_4867;
Future studies will investigate the effects of DRF on other adverse effects of opioids, including abuse liability, respiratory depression, and withdrawal. These promising results suggest that DRF has opioid-sparing properties.; Grant Support: NIH 1RF1NS113840-01
- |||||||||| ketamine / Generic mfg.
Strategically-substituted agmatines prevent the development of oxycodone self-administration (Virtual Only) - Dec 20, 2021 - Abstract #Neuroscience2021Neuroscience_4796;
While morphine and ketamine developed symptoms consistent with dependence associated with their respective receptor classes, the SSA compounds showed no indication of overt dependence behaviors representative of either class of compound. These data indicate that a strategically-substituted agmatine compound is capable of significantly reducing opioid-seeking behavior with a wide therapeutic window, avoiding the motor impairment and cardiovascular impairment typical of drugs of this class.; Grant Support: MR141337 and PR181740 from the Department of Defense; College of Pharmacy of the University of Minnesota
- |||||||||| morphine sulphate / Generic mfg., haloperidol / Generic mfg.
Sigma 1 antagonist haloperidol reduces tolerance induced by morphine in rats with neuropathic pain (Virtual Only) - Dec 20, 2021 - Abstract #Neuroscience2021Neuroscience_4705;
The interaction between sigma-1 receptor and the opioid system opens a window in neuropathic pain treatment. This study showed that haloperidol potentiates the antinociceptive effects of morphine and, in addition, modulates the tolerance induced by the opioid.
- |||||||||| morphine sulphate / Generic mfg.
The pathway of cMyc-EZH2-SIRT3 plays an important role in HIV-related neuropathic pain model (Virtual Only) - Dec 20, 2021 - Abstract #Neuroscience2021Neuroscience_4703;
Morphine increases c-Myc expression...ChIP assay showed that the enrichment of H3K27me3 binding to sirt3 gene promoter region was increased in gp120/M treated rats compared to that in control group. These results demonstrated that spinal cMyc-EZH2-H3K27me3-Sirt3 pathway played an important role in the HIV gp120-related neuropathic pain state, and provided a new insight into novel therapy for HIV/opioid-related neuropathic pain.; Grant Support: NIH Grant R01DA047157-03S1; NIH Grant R01DA047089; NIH Grant R01DA047157; NIH Grant R01DA34749
- |||||||||| morphine sulphate / Generic mfg.
Mrgprc11+ drg sensory neurons mediate glabrous skin itch (Virtual Only) - Dec 20, 2021 - Abstract #Neuroscience2021Neuroscience_4689;
To confirm this specificity, we also used morphine as an analgesic...These results suggest that MrgprC11+ neurons are a major mediator of glabrous skin itch. In summary, our findings reveal new avenues for future glabrous skin itch study and the development of glabrous-skin specific anti-itch therapies.; Grant Support: Pfizer Aspire Dermatology Award; NIH Grant HL141269; NIH Grant NS087088
- |||||||||| morphine sulphate / Generic mfg.
Systematic Isoform-Selective Heat Shock Protein 90 Inhibitors Improve the Therapeutic Index of Morphine (Virtual Only) - Dec 20, 2021 - Abstract #Neuroscience2021Neuroscience_4222;
Additionally, we found that KUNG65 and KUNB106 injection could rescue established morphine tolerance in the tail flick assay, again as we found for direct spinal cord injection. These results support our hypothesis that isoform-selective Hsp90 inhibitors can engage Hsp90 in the spinal cord when given systemically, resulting in improved opioid antinociception and side effects; strongly suggesting that Hsp90 isoform-selective inhibitors could be a powerful new tool to improve opioid therapy through a dose-reduction strategy, and further show that this effect can be achieved through a translationally relevant dosing route.; Grant Support: University of Arizona - institutional funds; Arizona Biomedical Research Commission New Investigator Award #ADHS18-198875
- |||||||||| morphine sulphate / Generic mfg.
Inhibition of Spinal Cord Hsp90 Enhances Src Kinase and Protein Kinase C signaling to Increase Opioid Anti-Nociception (Virtual Only) - Dec 20, 2021 - Abstract #Neuroscience2021Neuroscience_4217;
When inhibiting Hsp90 in the spinal cord through intrathecal (IT) administration of 17-N-allylamino-17-demethoxygeldanamycin (17-AAG) in mice we observed a significant increase in morphine anti-nociception, which could enable an opioid dose-reduction strategy...CRISPR knockdown of the Src Kinase also blocked enhanced antinociception in tail flick behavior. These findings have helped elucidate two key molecular mechanisms by which Hsp90 regulates opioid signaling and enhances opioid antinociception, creating potential targets to improve opioid treatment.; Grant Support: UA Institutional Funds; Arizona Biomedical Research Commission New Investigator Award #ADHS18-198875
- |||||||||| morphine sulphate / Generic mfg.
Adenylyl cyclase 1 involvement in chronic pain and morphine tolerance in mice (Virtual Only) - Dec 20, 2021 - Abstract #Neuroscience2021Neuroscience_4216;
Morphine induced hypersensitivity was attenuated after Adcy1 shRNA or pharmaceutical inhibition of AC1. Loss of Adcy1 activity decreases morphine tolerance and opioid-induced hypersensitivity, which could form the basis for novel therapeutics in the future.; Grant Support: K01 DA042902; Purdue University College of Pharmacy
- |||||||||| morphine sulphate / Generic mfg.
Assessing the synergistic effects of morphine and MP-III-024 co-administration: enhanced antinociception with reduced side effects (Virtual Only) - Dec 20, 2021 - Abstract #Neuroscience2021Neuroscience_4214;
Ongoing studies are evaluating the effects of MP-III-024/morphine co-administration on tolerance in the hot plate test and in conditioned place preference. These experiments are the first comprehensive preclinical analyses of a dual MOR-α2/α3GABAA pharmacotherapy strategy which may increase the therapeutic window between desirable opioid analgesic effects and side effects.; Grant Support: NIH DA043204; NIH NS076517; Milwaukee Institute for Drug Discovery; NIH DA041560; Camille and Henry Dreyfus Foundation (BL-19-004); internal funds from Rowan University and Cooper Medical School of Rowan University; University of Wisconsin-Milwaukee’s Shimadzu Laboratory for Advanced and Applied Analytical Chemistry; NSF CHE-1625735
- |||||||||| morphine sulphate / Generic mfg.
Resolving Delta Opioid Receptor Distribution and Function in Pain Neural Circuits (Virtual Only) - Dec 20, 2021 - Abstract #Neuroscience2021Neuroscience_4213;
Resolving Delta Opioid Receptor Distribution and Function in Pain Neural Circuits The delta opioid receptor (DOP) is an attractive drug target to develop novel opioid analgesics with fewer side effects, compared to mu opioid receptor (MOP) agonists such as morphine...Thus, in the adult DRG, Cre activity is largely restricted to mechanosensory neurons, as evidenced by the presence of tdTomato+ and GFP+ large diameter DRG neurons with axons that project both to spinal laminae III-IV and to medullary cuneate and gracile nuclei through the dorsal column pathway. Collectively, our data establish and validate the utility of Oprd1 Cre mice to investigate and manipulate DOP-expressing neurons, and begin to define the temporal and spatial characteristics of Oprd1 and Oprm1 (co)-expression for elucidating the functional organization of the opioid system.; Grant Support: T32DA035165; DoD Neurosensory Research Award W81XWH-15-1-0076; HHMI medical research fellowship; New York Stem Cell Foundation – Robertson Investigator Award; NIH fellowships T32GM089626; by NIH grant R01DA044481
- |||||||||| morphine sulphate / Generic mfg.
Mapping CNS correlates of pain processing, opioid tolerance, and pain processing in the tolerance setting (Virtual Only) - Dec 20, 2021 - Abstract #Neuroscience2021Neuroscience_4212;
This study aims to assess alterations in the patterns of neuronal activity in opioid tolerance and after acute noxious stimulation in morphine-tolerant mice...Preliminary results show unique patterns of activity in response to formalin in tolerant compared to non-tolerant mice, in several areas of the brain involved in pain modulation, notably the periaqueductal gray. Ongoing studies are also comparing the patterns of neuronal activity manifest in the opioid tolerant CNS with patterns generated in different chronic pain conditions, with the eventual goal of mapping anatomical regions and circuits that contribute to opioid- and opioid tolerance-dependent mechanisms of chronic pain development.; Grant Support: NIH R35 #NS097396 (AIB); Open Philanthropy
- |||||||||| morphine sulphate / Generic mfg.
Hiv-1 tat influences morphine reward and voluntary oral morphine consumption among ovariectomized female mice (Virtual Only) - Dec 20, 2021 - Abstract #Neuroscience2021Neuroscience_3590;
Differences in the neurocircuitry of brain regions involved in drug of abuse reward and reinforcement will be sought. Thus, these data demonstrate that HIV-1 Tat protein enhances the rewarding properties of morphine while reducing the demonstration of reinforcing behavior.; Grant Support: NIH Grant R00 DA039791; The University of Mississippi School of Pharmacy; NIH Grant R01 DA052851
- |||||||||| morphine sulphate / Generic mfg.
Targeting regulatory T cells to treat nerve injury-induced dynamic mechanical allodynia (Virtual Only) - Dec 20, 2021 - Abstract #Neuroscience2021Neuroscience_3573;
Delaying the initiation of ld-IL2 treatment to 7 days post-injury significantly attenuated the morphine-resistant punctate and dynamic allodynia at 3-5 weeks post-SNI...In contrast, ld-IL2 significantly increased the frequency of Treg cells among total CD3+ T cells in the injured sciatic nerves but not in the uninjured nerves or the DRG at 30 days post-SNI, suggesting the injured nerve as ld-IL2's site of action. Collectively, the present study identifies Treg cell as a promising target and ld-IL2 as a potential therapy for nerve injury-induced persistent punctate and dynamic mechanical allodynia that are highly debilitating and are largely unresponsive to available treatments.; Grant Support: NS103350- 02S1; Xiangya Scholarship Fund
- |||||||||| morphine sulphate / Generic mfg.
Tlr4 antagonism ameliorates persistent post-operative cognitive dysfunction (Virtual Only) - Dec 20, 2021 - Abstract #Neuroscience2021Neuroscience_3490;
Treating the rats with morphine, often administered post-operatively for pain management, exaggerated and prolonged this surgery-induced inflammatory response, leading to persistent POCD lasting at least 8 weeks...These results indicate that TLR4 plays a mediating role in the development of persistent POCD, although the specific mechanisms of this process remain to be elucidated. These data also suggest that blocking central TLR4 activation may be a promising therapeutic to treat POCD.; Grant Support: NIH Grant RF1AG028271
- |||||||||| morphine sulphate / Generic mfg.
Effects of photobiomodulation light therapy (PBM) on traumatic brain injury-induced opioid seeking (Virtual Only) - Dec 20, 2021 - Abstract #Neuroscience2021Neuroscience_2753;
At 30 days post-TBI, voluntary consumption was measured in a two-bottle choice, 24-hour free access model with initial access (four daily sessions) to morphine (0.1 mg/ml in 0.2% sucrose) opposed to a quinine (0.02 mg/ml in 0.2% sucrose) solution, during which average morphine consumption was comparable across injury groups (sham: 16.4 ± 2.1 mg/kg; TBI: 15.4 ± 1.8 mg/kg)...Together, these data suggest that PBM is effective in reducing post-TBI induced opioid seeking behavior and may represent an effective treatment for opioid misuse after TBI in the clinical population. Supported by facilities at the Detroit VA Medical Center, VA awards RX-003198 (KEB) and RX-003267 (ACC), the Richard Barber Interdisciplinary Research Program (JIM, ACC) and the Wayne State University Undergraduate Research Opportunities Program (NA, ACC).; Grant Support: Richard Barber Interdisciplinary Research Program (JIM and ACC); Detroit VA Medical Center, VA award RX-003198 (KEB); Detroit VA Medical Center, VA award RX-003267 (ACC); Wayne State University Undergraduate Research Opportunities Program UROP (NA, ACC)
- |||||||||| morphine sulphate / Generic mfg.
Role of Divalent Metal Transporter 1 in Morphine-Mediated Upregulation of Neuronal Ferritin Heavy Chain (Virtual Only) - Dec 20, 2021 - Abstract #Neuroscience2021Neuroscience_2615;
To further validate the contribution of DMT1, we are currently testing the effect of a specific genetic inhibition through viral-mediated silencing of DMT1 in cortical neurons. Overall, these studies suggest that DMT1 is involved in morphine-mediated regulation of CNS iron metabolism and that therapeutic interventions targeting the brain iron metabolism might be beneficial for individuals affected by neurocognitive disorders.; Grant Support: DA32444; DA15014
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