- |||||||||| Journal: Redox modulator iron complexes trigger intrinsic apoptosis pathway in cancer cells. (Pubmed Central) - May 27, 2024
Subsequent leakage of mitochondrial cytochrome c activates the caspase cascade to trigger the intrinsic apoptosis pathway in cancer cells. This strategy could be applied to leverage the inherent iron overload in cancer cells to selectively promote intrinsic cellular apoptosis for the development of unique iron-complex-based anticancer therapeutics.
- |||||||||| Journal: Dopamine?iron homeostasis interaction rescues mitochondrial fitness in Parkinson's disease. (Pubmed Central) - May 18, 2024
Importantly, dopamine treatment of PD neurons promoted a rescue effect by increasing mitochondrial respiration, activating antioxidant stress response, and normalizing altered metabolite levels linked to mitochondrial function. These observations provide evidence that dopamine affects iron homeostasis, intracellular stress responses and mitochondrial function in healthy cells, while dopamine supplementation can restore the disturbed regulatory network in PD cells.
- |||||||||| Journal: AFG3L2 and ACO2-linked Dominant Optic Atrophy: genotype-phenotype characterization compared to OPA1 patients. (Pubmed Central) - May 16, 2024
Clinically, DOA remains a fairly homogeneous entity despite the growing genetic heterogeneity. ACO2 seems to be associated with an overall better preservation of retinal ganglion cells, probably depending on the different pathogenic mechanism involving mtDNA maintenance, as opposed to AFG3L2, which is involved in OPA1 processing and is virtually indistinguishable from classic OPA1-DOA.
- |||||||||| Journal: MEMO1 binds iron and modulates iron homeostasis in cancer cells. (Pubmed Central) - May 9, 2024
Our work reveals that the iron coordination mode in MEMO1 is very similar to that of iron-containing extradiol dioxygenases, which also display a similar structural fold. We conclude that MEMO1 is an iron-binding protein that modulates iron homeostasis in cancer cells.
- |||||||||| A Pilot Study: The Possible Roles Of Exercise-induced Frataxin Expression In Skeletal Muscle (327; Hynes CC-Hall A) - May 4, 2024 - Abstract #ACSM2024ACSM_3072;
This pilot study suggests that exercise training may contribute to heme biosynthesis in skeletal muscle via frataxin. Nevertheless, follow-up studies are warranted to further understand the roles of frataxin on the heme system, as well as mitochondrial capacity in exercise-induced muscle adaptations.
- |||||||||| Journal: Influences of Ipomoea batatas Anti-Cancer Peptide on Tomato Defense Genes. (Pubmed Central) - May 3, 2024
Nevertheless, follow-up studies are warranted to further understand the roles of frataxin on the heme system, as well as mitochondrial capacity in exercise-induced muscle adaptations. IbACP enhances the synthesis of defense hormones and up-regulates downstream defense genes, improving the plant's resistance to biotic stresses.
- |||||||||| Review, Journal: HFE Mutations in Neurodegenerative Disease as a Model of Hormesis. (Pubmed Central) - Apr 1, 2024
This review describes the current research regarding the contribution of HFE variants to neurodegenerative disease prognosis in the context of a hormetic model. To our knowledge, this is the first time that a hormetic model for neurodegenerative disease has been presented.
- |||||||||| Journal: Application of L-Cysteine Hydrochloride Delays the Ripening of Harvested Tomato Fruit. (Pubmed Central) - Mar 28, 2024
Furthermore, transcriptome analysis revealed that a substantial number of genes related to ethylene biosynthesis (ACS2, ACS4, ACO1, ACO3), carotenoid biosynthesis (PSY, PDS, ZDS, CRTISO), cell wall degradation (PG1/2, PL, TBG4, XTH4), and ripening-related regulators (RIN, NOR, AP2a, DML2) were downregulated by LCH, resulting in delayed ripening. These findings suggest that the application of LCH delays the ripening of harvested tomato fruit by modulating the redox balance and suppressing the expression of ripening-related genes.
- |||||||||| TCA Cycle Metabolons in Cardiac Mitochondria (Convention Center Exhibit Hall) - Mar 16, 2024 - Abstract #PAS2024PAS_2993;
Bands with signals of aconitase (ACON), isocitrate (IDH) or malate dehydrogenase (MDH) activity were excised from the native gel and the proteins were separated by SDS electrophoresis/immunoblotting. A protein complex of about 1000 kD contained MDH, citrate synthase (CS), IDH, and ACON but not alpha-ketoglutarate dehydrogenase or succinate dehydrogenase.
- |||||||||| Journal, IO biomarker: Attenuation of HOIL-1L ligase activity promotes systemic autoimmune disorders by augmenting linear ubiquitin signaling. (Pubmed Central) - Feb 8, 2024
We found that the R464H variant, which is encoded by rs774507518 within the RBCK1/HOIL-1L gene, attenuated HOIL-1L ligase activity and augmented LUBAC-mediated immune signaling, including that mediated by Toll-like receptors. We also found that rs774507518 was enriched significantly in patients with SLE, strongly suggesting that RBCK1/HOIL-1L is an SLE susceptibility gene and that augmented linear ubiquitin signaling generated specifically by LUBAC underlies the pathogenesis of this prototype systemic autoimmune disease.
- |||||||||| Journal: Epidermal turnover and iron metabolism in senile lentigo. (Pubmed Central) - Jan 13, 2024
These changes in the expression of molecules involved in iron uptake/export/utilization might reflect the altered iron utilization state in SL, resulting in disruption of keratinocyte differentiation and disturbing epidermal turnover. Our results suggest that the metabolism of iron in keratinocytes in SL differs from that in the normal epidermis, and these changes could be associated with the abnormal epidermal turnover and decreased melanin excretion in SL.
- |||||||||| Journal: Small extracellular vesicle-based human melanocyte and melanoma signature. (Pubmed Central) - Jan 2, 2024
Results reveal the presence of unique protein signatures for melanocytes and melanoma cells that reflect cellular transformation-related profound modifications. Melanocyte-derived sEVs are enriched in oxidative metabolism (e.g., aconitase 2, ACO2) or pigmentation (e.g., tyrosinase, TYR) related proteins while melanoma-derived sEVs reflect a generalized decrease in mature melanocytic markers (e.g., melanoma antigen recognized by T-cells 1, MART-1, also known as MLANA) and an increase in epithelial to mesenchymal transition (EMT)-related adhesion molecules such as tenascin C (TNC).
- |||||||||| Journal: Metagenomic and metaproteomic analyses of microbial amino acid metabolism during Cantonese soy sauce fermentation. (Pubmed Central) - Nov 30, 2023
Kyoto Encyclopedia of Genes and Genomes analysis revealed that the main enzymes involved in the metabolism of umami amino acids were aspartate aminotransferase, citrate synthase, aconitase, and isocitrate dehydrogenase, which were produced by Z. rouxii and A. oryzae during early fermentation (0-15 d) and the middle fermentation stage (60-90 d). This study constructed a regulatory network of enzymes potentially involved in the metabolism of flavor amino acids, which provided a theoretical basis for studying the amino acid metabolism of Cantonese soy sauce.
- |||||||||| Journal: Investigation of the activity of a novel tropolone in osteosarcoma. (Pubmed Central) - Nov 14, 2023
Collectively, we demonstrate that MO-OH-Nap-induced cytotoxic effects in OS cells are dependent on the tropolone's ability to alter cellular iron availability and that this agent exploits key metabolic pathways. These studies support further evaluation of MO-OH-Nap as a novel treatment for OS.
- |||||||||| GENOME-WIDE MUTAGENESIS SCREENS IDENTIFY REGULATORS OF CELLULAR IRON METABOLISM AND FERROPTOSIS (Windsor, 5th floor) - Nov 11, 2023 - Abstract #BTSWM2023BTS_WM_186;
Conclusions We establish a robust reporting system for intracellular iron levels in patient-derived lung cancer cell lines, definitively map the cellular pathways of iron metabolism, identify novel regulators of iron homeostasis, and determine their impact on the induction of ferroptosis. SETD2 loss of function mutations, which are common in cancer, may promote resistance to ferroptosis, with potentially important implications for future therapies targeting this cell death pathway.
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