- |||||||||| cyclosporin A microemulsion / Generic mfg.
Mapping natural killer cell contributions to antibody-mediated rejection of kidney allografts and responsiveness to immunosuppression (Grand Georgian) - Mar 10, 2023 - Abstract #ITS2023ITS_32;
To assess the impact of immunosuppression, we are systematically examining effects of mycophenolic acid, cyclosporine A, and prednisolone alone or in combination on NK cell proliferation, cytotoxicity, and cytokine production...Conclusions : Collectively these studies are mapping NK cells in ABMR and identifying mechanisms by which NK cells contribute to pathology. Ongoing studies seek to identify novel approaches to limit NK cell activity in ABMR, informed by patient-based scRNAseq studies.
- |||||||||| Identification of Novel Drug Targets and Exploring Drug Repurposing Possibilities for IgA Nephropathy () - Mar 9, 2023 - Abstract #ISPOR2023ISPOR_1585;
To date, there are only few effective treatments that help in the management of the progression of IgA nephropathy and its complications. Therefore, this hypothesis could serve as the basis for further in-vitro and in-vivo studies revealing newer drug options for IgA nephropathy and potentially bypassing the extensive work, time and expense involved in the initial stages of de-novo drug design.
- |||||||||| Journal: The Genetic and Immunologic Landscape Underlying the Risk of Malignant Progression in Laryngeal Dysplasia. (Pubmed Central) - Feb 26, 2023
TILs showed a highly positive correlation with CCR6, CD83, HLA-DPB1, MX1 and SNAI1, and they were inversely correlated with CD48, CIITA, CXCR4, FCER1G, IL1B, LST1 and TLR8. (4) TILs have a great potential to identify high-risk progression dysplasia and thus to define surveillance protocols and prevention programs.
- |||||||||| Journal: Dysfunctional network of hub genes in hypertrophic cardiomyopathy patients. (Pubmed Central) - Jan 12, 2023
Our study revealed that ten hub genes (CD14, ITGB2, C1QB, CD163, HCLS1, ALOX5AP, PLEK, C1QC, FCER1G, and TYROBP) are involved in the development and progression of HCM. These genes can potentially be used as biomarkers and therapeutic targets for HCM patients.
- |||||||||| Journal: Detailed phenotypic and functional characterization of CMV-associated adaptive NK cells in rhesus macaques. (Pubmed Central) - Dec 16, 2022
These data demonstrate the evolutionary conservation of the CMV-driven development of NKG2C-positive adaptive NK cells with particular molecular signatures in primates and with changes in gene copy numbers and ligand-binding strength of NKG2C isotypes. Thus, rhesus macaques represent a suitable and valuable nonhuman primate animal model to study the CMV-NKG2C liaison in vivo.
- |||||||||| Journal, IO biomarker, Pan tumor: Prognostic and immunological role of FCER1G in pan-cancer. (Pubmed Central) - Nov 5, 2022
FCER1G can be used to predict the efficacy of immunological checkpoint therapy among these types of tumors patients. Our study also provides an important basis for the clinical use of FCER1G to assess the patient immune status and the selection of individualized immunotherapy options.
- |||||||||| Opdivo (nivolumab) / Ono Pharma, BMS
ZNF683 (Hobit) Marks a CD8+ T Cell Population Associated with Anti-Tumor Immunity Following Anti-PD-1 Therapy for Richter Syndrome (ENMCC - La Nouvelle Orleans Ballroom AB) - Nov 4, 2022 - Abstract #ASH2022ASH_5101;
We again identified ZNF683 among the top upregulated genes in RS-R (Log2 fold change = 2.13, p=0.037), along with other genes enriched in our ZNF683high CD8 E/EM signature (BATF, CORO1A, CD38, ITGB2, GZMM), while RS-NR were enriched in NK-like genes (KLRC1, FCGR3A, KLRG1, KLRF1 and FCER1G). In conclusion, we identify ZNF683 as marking a CD8 T cell population associated with CPB response in RS blood and bone marrow and provide evidence that this transcription factor regulates key cellular pathways involved in immune anti-tumor response, with implications for understanding CPB response in RS and other malignancies.
- |||||||||| metformin / Generic mfg.
Preclinical, Journal: Microarray analysis of mRNA expression profiles in liver of ob/ob mice with real-time atmospheric PM exposure. (Pubmed Central) - Oct 22, 2022
Our study was aimed to explore the impact of PM on the transcriptome level in the liver of ob/ob mice by atmosphere PM whole-body dynamic exposure system, and meanwhile preliminarily investigated the effects of metformin intervention in this process...They were related to insulin resistance, glucose and lipid metabolism and other liver metabolism, and also neurodegenerative diseases. This study provided valuable clues and possible protective measures to the liver damage in ob/ob mice caused by PM exposure, and further research is needed to explore the related mechanism in detail.
- |||||||||| Biomarker, Journal: Identification of the Immune Status of Hypertrophic Cardiomyopathy by Integrated Analysis of Bulk- and Single-Cell RNA Sequencing Data. (Pubmed Central) - Oct 21, 2022
S100A9, TYROBP, FCER1G, CD14, and S100A8 were identified as potential biomarkers to discriminate HCM from the control group. S100a9, TYROBP, FCER1G, and S100a8 were mainly expressed by infiltrated M1 proinflammatory cells, especially Ccr2-M1 proinflammatory cells in the heart immune microenvironment while Cd14 was expressed by M2 macrophages in transverse aortic constriction (TAC) mice at 1 week.
- |||||||||| Strengths and Limitations of Integrating Single Cell and Spatial Transcriptomics to Study T Cells in Normal and AKI Kidneys (Exhibit Hall, Orange County Convention Center, West Building) - Oct 13, 2022 - Abstract #KIDNEYWEEK2022KIDNEY_WEEK_1019;
Conclusion Single cell RNAseq is a useful discovery technique to study T cell gene expression in normal and AKI kidneys, and can be validated in human samples. However, spatial transcriptomics at the current technology has resolution limitations to study kidney T cells, but is useful for epithelial cell, macrophage, neutrophil, cytokine and chemokine studies.
- |||||||||| In vivo programming of myeloid cells by mRNA mediated delivery of novel Fca fusion receptor activates anti-tumor immunity (Hall C) - Oct 6, 2022 - Abstract #SITC2022SITC_1545;
Furthermore, in the B16 syngeneicinc melanoms melanoma model, treatment with the melanoma antigen GP75 targeted Fca Receptor Fusion Constructs was also associated with the the initiation intiatation of broad systemic immune responses, characterized by tumoral accumulation of activated CD8+ T cells, reduced tumor associated Tregs and activation of antigen presenting cells in spleen. Together these studies highlight the potential of Fca Receptor Fusion Construct delivered directly in vivo to program myeloid cells to recognize and kill cancer.
- |||||||||| Journal: Phosphorylation on Y342 Syk is important for both ITAM and hemITAM signaling in platelets. (Pubmed Central) - Sep 10, 2022
Hemostasis, as measured by tail bleeding time, was not altered in Syk Y342F mice, but thrombus formation in response to FeCl injury was prolonged in Syk Y342F mice. These data demonstrate that phosphorylation of Y342 on Syk following stimulation of either GPVI or CLEC-2 receptors is important for the ability of Syk to transduce a signal.
- |||||||||| Journal: Spatial transcriptomics identifies enriched gene expression and cell types in human liver fibrosis. (Pubmed Central) - Sep 3, 2022
Deconvolution and enumeration of parenchymal and fibrotic gene content as measured by spatial transcriptomics into distinct cell states revealed significantly higher frequencies of ACTA2+ FABP4+ and COL3A1+ mesenchymal cells, IL17RA+ S100A8+ and FCER1G+ tissue monocytes, VCAM1+ SDC3+ Kupffer cells, CCL4+ CCL5+ KLRB1+ and GZMA+ IL17RA+ T cells and HLA-DR+, CD37+ CXCR4+ and IGHM+ IGHG+ B cells in fibrotic liver regions compared with parenchymal areas of cirrhotic explants. Our findings indicate that spatial transcriptomes of parenchymal and fibrotic liver regions express unique gene content within cirrhotic liver and demonstrate proof of concept that spatial transcriptomes combined with additional RNA sequencing methodologies can refine the localization of gene content and cell lineages in the search for antifibrotic targets.
- |||||||||| Journal: FCER1G Gene Hypomethylation in Patients with Rheumatoid Arthritis. (Pubmed Central) - Aug 27, 2022
We observed that RA patients have lower levels of methylation of the FCER1G gene compared to controls, but we did not find any difference in the methylation status of this gene between patients with high disease activity and remission. The results of this study suggest that FCER1G gene methylation may be a new potential epigenetic marker of RA that is independent of disease activity.
- |||||||||| Pretransplant kidney transcriptome captures immune pathways and predicts 24-month outcomes (Hall E) - Aug 4, 2022 - Abstract #TTS2022TTS_47;
A predictive risk score was developed, which combines donor age, race, BMI, and donor quality gene markers, and can be calculated prior to transplantation to predict graft function. Differential pretransplant transcriptional profiles between kidneys with low and high function at 24-months were also identified, providing a deeper insight into the early biological processes leading to graft dysfunction.
- |||||||||| Review: RNAseq of Osteoarthritic Synovial Tissues: Systematic Literary Review. (Pubmed Central) - Jul 14, 2022
These differentially expressed microbes have also been linked to the inflammatory pathway. Further investigation with more clinical gene profiling in synovial tissue of OA subjects is required to reveal the causation and progression, as well as aid in the development of new treatments.
- |||||||||| Journal: A Self-reactive Innate-like T-cell Program Was Identified in Cancer Immunity. (Pubmed Central) - Jul 9, 2022
Further investigation with more clinical gene profiling in synovial tissue of OA subjects is required to reveal the causation and progression, as well as aid in the development of new treatments. A class of αβ T-cell receptor-positive, FCER1G-expressing T cells with high cytotoxic potential was identified.
- |||||||||| Biomarker, Journal: Immune Cell Infiltration Characteristics of Pigmented Villous Nodular Synovitis and Prediction of Potential Diagnostic Markers Based on Bioinformatics. (Pubmed Central) - Jun 22, 2022
Furthermore, our correlation analysis of diagnostic markers and infiltrating immune cells found that TYROBP, FCER1G, LAPTM5, and HLA-DPB1 were positively correlated with M2 macrophage infiltration and that neutrophils, TYROBP, FCER1G, and LAPTM5 were negatively correlated with plasma cell infiltration. We identified TYROBP, FCER1G, LAPTM5, and HLA-DPB1 as potential diagnostic markers for PVNS and concluded that immune cell infiltration plays an important role in the genesis and progression of PVNS.
- |||||||||| Single cell atlas of cd45+ cells in angiotensin II-induced hypertension (Research Gateway 5) - Jun 15, 2022 - Abstract #ESC2022ESC_2963;
Comprehensive single-cell RNA sequencing identifies tissue-resident macrophages, monocyte-derived dendritic cells, and NK cells as most affected leukocyte subpopulations in hypertensive vasculature. Differentially expressed genes support the role of these cells in vascular remodelling and propagation of inflammation, further supporting the identification of these cells as potential future targets for therapeutic interventions in hypertension.
- |||||||||| Journal: Key Genes Associated With Non-Alcoholic Fatty Liver Disease and Polycystic Ovary Syndrome. (Pubmed Central) - Jun 14, 2022
Then, four miRNAs, including miR-20a-5p, miR-129-2-3p, miR-124-3p, and miR-101-3p, were predicted as possibly the key miRNAs through the miRNA-gene network construction. In summary, we firstly constructed a miRNA-gene regulatory network depicting interactions between the predicted miRNA and the hub genes in NAFLD and PCOS, which provides novel insights into the identification of potential biomarkers and valuable therapeutic leads for PCOS and NAFLD.
- |||||||||| Biomarker, Journal: Exploration of Potential Biomarker Genes and Pathways in Kawasaki Disease: An Integrated in-Silico Approach. (Pubmed Central) - May 29, 2022
In addition, the pathway analysis also indicated that the majority of the DEGs in KD were enriched in systemic lupus erythematosus, suggesting a strong interplay between immunological and genetic factors in the pathogenesis of KD. These findings could significantly aid in identifying therapeutic targets and understanding KD biosignatures to design a biomarker panel for early diagnosis and severity of the disease.
- |||||||||| Journal, IO biomarker: Programme of self-reactive innate-like T cell-mediated cancer immunity. (Pubmed Central) - May 7, 2022
Notably, expansion and effector differentiation of intratumoural ILTCKs depended on interleukin-15 (IL-15) expression in cancer cells, and inducible activation of IL-15 signalling in adoptively transferred ILTCK progenitors suppressed tumour growth. Thus, the antigen receptor self-reactivity, unique ontogeny, and distinct cancer cell-sensing mechanism distinguish ILTCKs from conventional cytotoxic T cells, and define a new class of tumour-elicited immune response.
- |||||||||| Clinical, Journal: Transcriptome analysis reveals intrinsic pro-inflammatory signaling in healthy African American skin. (Pubmed Central) - Apr 27, 2022
Finally, AA-specific DEGs in skin and HSE significantly overlapped with molecular signatures of skin in AD and psoriasis patients. Overall, these findings suggest the existence of intrinsic pro-inflammatory circuits in AA keratinocytes/skin that may account for disease disparities and will help to build a foundation for the development of targeted skin disease prevention.
- |||||||||| Journal: Allele-specific analysis reveals exon- and cell-type-specific regulatory effects of Alzheimer's disease-associated genetic variants. (Pubmed Central) - Apr 26, 2022
In an attempt to pinpoint the cell types responsible for the observed tissue-level aseQTLs using the snRNA-seq data, we detected many aseQTLs in microglia or monocytes associated with immune-related genes, including HLA-DQB1, HLA-DQA2, CD33, FCER1G, MS4A6A, SPI1, and BIN1, highlighting the regulatory role of AD-associated variants in the immune response. These findings provide further insights into potential causal pathways and cell types mediating the effects of the AD-associated variants.
- |||||||||| Journal: Integrated Bioinformatics Analysis and Verification of Gene Targets for Myocardial Ischemia-Reperfusion Injury. (Pubmed Central) - Apr 26, 2022
Emr1, Tyrobp, Itgb2, Fcgr2b, Cybb, and Fcer1g might be critical in the pathological process of MIRI, and the natural products (araloside C, glycyrrhizic acid, ophiopogonin D, polyphyllin I, and punicalagin) targeting these hub genes exhibited potential therapeutic efficacy on MIRI. Our findings provided new insights to explore the mechanism and treatments for MIRI and revealed new therapeutic targets for natural products with protective properties against MIRI.
|
