A1CF inhib 
Welcome,         Profile    Billing    Logout  
 0 Companies  0 Products   0 Products   0 Diseases   0 Trials   0 News 


1234»
  • ||||||||||  Journal:  Engineered CBEs based on Macaca fascicularis A3A with improved properties for precise genome editing. (Pubmed Central) -  Mar 3, 2024   
    In contrast, a chimeric BE4-hA3A-SVR+ shows editing efficiency increased by about 50%, with other properties unaffected. Our findings demonstrate that mA3A-based CBEs could provide prototype options with advantages over rA1- and hA3A-based CBEs for further optimization, highlighting the importance of the SVR motif in defining CBE intrinsic properties.
  • ||||||||||  Journal:  Improvement of C-to-U RNA editing using an artificial MS2-APOBEC system. (Pubmed Central) -  Jan 30, 2024   
    Moreover, the editing efficiency of APOBEC3G can be enhanced by site-specific mutation to alter the base preference. Overall, our results demonstrate that the MS2 system can fuse and catalyze reactions with different enzymes, suggesting that it holds an even greater potential for RNA editing than is utilized currently.
  • ||||||||||  Journal:  Unraveling C-to-U RNA editing events from direct RNA sequencing. (Pubmed Central) -  Dec 17, 2023   
    Using in vitro synthesized and human ONT reads, our model optimizes the signal-to-noise ratio improving the detection of C-to-U editing sites with high accuracy, over 90% in all samples tested. Our results suggest that iForest, known for its rapid implementation and minimal memory requirements, is a promising tool to denoise ONT reads and reliably identify RNA modifications.
  • ||||||||||  Journal:  RNA molecular recording with an engineered RNA deaminase. (Pubmed Central) -  Dec 17, 2023   
    Furthermore, we identify transcript isoform-specific RNA-protein interactions driven by isoform changes distal to the binding site. The genetically encodable RNA deaminase rABE enables single-molecule identification of RNA-protein interactions with cell type specificity.
  • ||||||||||  cisplatin / Generic mfg.
    Journal:  APOBEC-1 deletion enhances cisplatin-induced acute kidney injury. (Pubmed Central) -  Dec 14, 2023   
    Overexpression of Apobec-1 in mouse proximal tubule cells protected against CP-induced cytotoxicity. These findings suggest that Apobec-1 mediates critical pro-survival responses to renal injury and increasing Apobec-1 expression could be an effective strategy to mitigate AKI.
  • ||||||||||  Journal:  Inflammation-induced TRIM21 represses hepatic steatosis by promoting the ubiquitination of lipogenic regulators. (Pubmed Central) -  Nov 8, 2023   
    Our study identifies TRIM21 as a negative regulator of liver steatosis in NASH and provides mechanistic insights into an immunometabolic crosstalk that limits fatty acid synthesis and fructose metabolism during metabolic stress. Thus, enhancing this natural counteracting force of steatosis through inhibition of key lipogenic activators via TRIM21-mediated ubiquitination may provide a therapeutic opportunity to treat NASH.
  • ||||||||||  m6a-Son-CCL5 Axis Controls HSC Fate and Inflammation (SDCC - Halls G-H) -  Nov 3, 2023 - Abstract #ASH2023ASH_1697;    
    Moreover, co-culturing WT HSCs with CCL5 resulted in a symmetric commitment defect similar to that observed with m 6 A loss. In summary, our study delineates the critical m 6 A-SON-CCL5 axis governing HSC symmetric commitment fate and inflammation control.
  • ||||||||||  Journal:  Cytosine base editors optimized for genome editing in potato protoplasts. (Pubmed Central) -  Sep 18, 2023   
    The CBEs were overall highly efficient, with A3A having the best overall base editing activity, with an average 34.5%, 34.5%, and 27% C-to-T conversion at C4, C5, and C12, respectively, whereas CDA1 showed an average base editing activity of 34.5%, 34%, and 14.25% C-to-T conversion at C4, C5, and C12, respectively. rA1 exhibited an average base editing activity of 18.75% and 19% at C4 and C5 and was the only base editor to show no C-to-T conversion at C12.
  • ||||||||||  Journal, Machine learning:  Recapitulation of patient-specific 3D chromatin conformation using machine learning. (Pubmed Central) -  Sep 8, 2023   
    Application of our model on 371 samples across 22 cancer types revealed 1,780 enhancer-gene connections for 602 cancer genes. Using CRISPR interference (CRISPRi), we validated enhancers predicted to regulate ESR1 in estrogen receptor (ER)+ breast cancer and A1CF in liver hepatocellular carcinoma.
  • ||||||||||  Transcriptional consequences of driver mutations in chronic liver disease (Poster and Exhibition Hall) -  Jun 13, 2023 - Abstract #EACR2023EACR_462;    
    We describe transcriptional signatures associated with driver mutations.ConclusionHere we demonstrate that clonal expansions promoted in the context of chronic lipid overload can dominate the clonal architecture of the entire organ, without malignant transformation. We show how metabolism is reprogrammed in mutant clones.
  • ||||||||||  Journal:  Perspective for Studying the Relationship of miRNAs with Transposable Elements. (Pubmed Central) -  May 15, 2023   
    In addition, the created database could become the basis for clarifying the mechanisms of pathogenesis of various diseases (imbalance in the activity of transposable elements, reflected in changes in the expression of miRNAs) and designing their targeted therapy using specific miRNAs as targets. This article provides examples of the detection of transposable elements-derived miRNAs involved in the development of specific malignant neoplasms, aging, and idiopathic pulmonary fibrosis.
  • ||||||||||  CHRONIC KIDNEY DISEASE-RELATED SINGLE NUCLEOTIDE POLYMORPHISMS (SNPs) AMONG ADULT WEST AFRICANS WITH AND WITHOUT HIV () -  Mar 28, 2023 - Abstract #ISNWCN2023ISN_WCN_1172;    
    For Model 2, rs11959928 ( DAB2 ) was associated with CKD [OR 0.21 (95% CI 0.04-1.00; p=0.05) while in Model 3, rs10994860 ( A1CF ) was associated with increased risk of CKD [OR 3.49 (95% CI 1.30-9.33); p=0.01]. Conclusions The SNPs rs4293393 ( UMOD), rs11959928 ( DAB2 ) and rs10994860 ( A1CF ) should be investigated further as candidate SNPs that are associated with CKD in the Black population of West Africa.
  • ||||||||||  Preclinical, Journal:  Introduction of Genetic Mutations Into Mice by Base Editor and Target-AID. (Pubmed Central) -  Feb 11, 2023   
    Base editing is a modified CRISPR/Cas system that induces base substitution at targeted genomic regions. Here I describe a basic protocol to introduce disease-relevant pathogenic mutations into mice utilizing two representative base editing tools, Base Editor and Target-AID.
  • ||||||||||  Journal:  APOBEC mutagenesis is a common process in normal human small intestine. (Pubmed Central) -  Jan 27, 2023   
    APOBEC1 mRNA levels were very high in the small intestine epithelium, but low in the large intestine epithelium and other tissues. The results suggest that the high levels of SBS2/SBS13 in the small intestine are collateral damage from APOBEC1 fulfilling its physiological function of editing APOB mRNA.
  • ||||||||||  ampicillin / Generic mfg.
    Journal:  CRISPR-based engineering of phages for in situ bacterial base editing. (Pubmed Central) -  Nov 15, 2022   
    Furthermore, using a multigenera synthetic soil community, we employ phage-assisted base editing to induce host-specific phenotypic alterations in a community context both in vitro and within the EcoFAB, observing editing efficiencies from 10 to 28% across the bacterial population. The concurrent use of a synthetic microbial community, soil matrix, and EcoFAB device provides a controlled and reproducible model to more closely approximate in situ editing of the soil microbiome.
  • ||||||||||  Journal:  Efficient multi-nucleotide deletions using deaminase-Cas9 fusions in human cells. (Pubmed Central) -  Oct 25, 2022   
    In addition, the C-DEL system generates a considerable fraction of predictable multi-nucleotide deletions from 5'-deaminated C bases to the Cas9-cleavage site and increases the proportion of larger deletions at the target loci. Taken together, the C-DEL and A-DEL systems provide a practical strategy for producing efficient multi-nucleotide deletions, expanding the CRISPR/Cas9 toolsets for gene modifications in human cells.
  • ||||||||||  Revealing Novel Signaling Pathways Affected in Glomeruli During Salt-Sensitive Hypertension (Exhibit Hall, Orange County Convention Center, West Building) -  Oct 13, 2022 - Abstract #KIDNEYWEEK2022KIDNEY_WEEK_3160;    
    Conclusion We revealed here novel gene networks affected in glomeruli during SS-HTN. Alignment of this data with other existing ‘omics analyses is necessary to provide further insight into the development of hypertensive glomerulosclerosis.
  • ||||||||||  cisplatin / Generic mfg.
    Apobec-1 Limits Cisplatin and Ischemia-Reperfusion-Induced AKI (Exhibit Hall, Orange County Convention Center, West Building) -  Oct 13, 2022 - Abstract #KIDNEYWEEK2022KIDNEY_WEEK_994;    
    The absence of Apobec1 in macrophages prevented the transition of a cell death promoting phenotype to one supporting repair and regeneration. Apobec1 is a critical pro-survival response to renal injury and increasing Apobec-1 activity could be an effective strategy to reduce or prevent AKI.
  • ||||||||||  Ribo-stamp - a new method for measuring translation in neurons at the single-cell level (SDCC Halls B-H) -  Oct 10, 2022 - Abstract #Neuroscience2022NEUROSCIENCE_2485;    
    Results from this study indicate that Ribo-STAMP can be used for cell-type identity mapping throughout the brain. Understanding the translational profiles of different neuronal subtypes will facilitate to provide insights into how the brain functions in a healthy state and how it may go awry during disease.
  • ||||||||||  Journal:  The roles of APOBEC-mediated RNA editing in SARS-CoV-2 mutations, replication and fitness. (Pubmed Central) -  Sep 16, 2022   
    Instead, expression of wild-type APOBEC3 greatly promotes viral replication/propagation, suggesting that SARS-CoV-2 utilizes the APOBEC-mediated mutations for fitness and evolution. Unlike the random mutations, this study suggests the predictability of all possible viral genome mutations by these APOBECs based on the UC/AC motifs and the viral genomic RNA structure.