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Review, Journal: Acute Effects of Nutritive and Non-Nutritive Sweeteners on Postprandial Blood Pressure. (Pubmed Central) - Jan 21, 2020
The limited studies investigating other nutritive and non-nutritive sweeteners have demonstrated minimal or no effect on postprandial BP. Dietary modification by replacing high nutritive sweeteners (glucose, fructose, and sucrose) with low nutritive (d-xylose, xylitol, erythritol, maltose, maltodextrin, and tagatose) and non-nutritive sweeteners may be a simple and effective management strategy for PPH.
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Review, Journal: Polyol dehydrogenases: intermediate role in the bioconversion of rare sugars and alcohols. (Pubmed Central) - Jan 1, 2020
In addition to MDH, the other PDHs including RDH, GDH, ArDH, and XDH are also important tools for the production of rare sugars including D-tagatose, allitol, D-xylulose, and L-xylulose. Concerning the intermediate function of PDH in the linkage of rare sugar and sugar alcohols, this review attempts to conclude their catalytic properties and potential applications.
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Journal: Bioconversion of Xylose to Ethylene Glycol and Glycolate in Engineered Corynebacterium glutamicum. (Pubmed Central) - Dec 25, 2019
A synthetic ribulose 1-phosphate (Ru1P) pathway involving heterologous d-tagatose 3-epimerase and l-fuculose kinase/aldolase reactions was introduced in C. glutamicum...In addition, the bioconversion of xylose to glycolate was achieved, and the almost maximum molar yield was 0.99 mol per mol xylose in C. glutamicum via the Ru1P pathway. Thus, the synthetic Ru1P pathway in C. glutamicum led bioconversion of xylose to either ethylene glycol or glycolate with high molar yields.
- |||||||||| Ac-225 rosopatamab tetraxetan (CONV01-?) / Dominari Holdings
Trial completion date, Metastases: 7R01CA207645-03: Phase I Trial of 225Ac-J591 in Patients With mCRPC (clinicaltrials.gov) - Nov 29, 2019
P1, N=42, Recruiting,
This is the first report on characteristic metabolic profiles and potential metabolite biomarkers in serum for reliable diagnosis of BD using GC/TOF-MS. Trial completion date: Dec 2020 --> Jul 2021
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Journal: In search for an alternative to sugar to reduce obesity. (Pubmed Central) - Oct 8, 2019
They do have the bulk and the mouth fill of table sugar with reduced caloric content (0.2 kcal/g for D-allulose). Additional randomized controlled trials are necessary to define the long term safety and efficacy of these sugars.
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Review, Journal: An overview on biological production of functional lactose derivatives. (Pubmed Central) - Aug 15, 2019
These lactose derivatives show a range of prominent physiological features and effects, such as prebiotic properties, indigestibility, and obesity prevention, which can be utilized in the pharmaceutical, health, and food industries. In this review, we present the properties and physiological effects of lactose derivatives, detailing their biological production by various enzymes and their applications in dairy products, especially directly in the milk industry.
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Journal, HEOR: Functionalized polyhydroxyalkanoate nano-beads as a stable biocatalyst for cost-effective production of the rare sugar d-allulose. (Pubmed Central) - Aug 15, 2019
...The functionalized polyhydroxyalkanoate (PHA) nano-beads decorated with d-tagatose 3-epimerase (DTE) was produced in recombinant endotoxin-free ClearColi, whereby the expression, purification, and immobilization of the active DTE were efficiently combined into one step...Moreover, the d-allulose/d-fructose binary mixture can be simply separated by a single cation exchange resin-equipped chromatography. Taken together, DTE-PHA beads are promising and robust nano-biocatalysts that will remarkably simplify the production procedures of d-allulose, contributing to its cost-effective production.
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Biomarker, Journal: Metabolomics identifies metabolite biomarkers associated with acute rejection after heart transplantation in rats. (Pubmed Central) - Jul 26, 2019
We assessed International Society for Heart and Lung Transplantation (ISHLT) grades 0, 3B, 4, 1 and 1 rejection in groups A to E. There were 15 differential metabolites between groups A and B, 14 differential metabolites between groups A and C, and 10 differential metabolites between groups B and C. In addition, four common differential metabolites, including D-tagatose, choline, C16 sphinganine and D-glutamine, were identified between on days 5 and 7 post-transplantation. Our findings demonstrate that the panel of D-tagatose, choline, C16 sphinganine and D-glutamine exhibits a high sensitivity and specificity for the early diagnosis of acute rejection after heart transplantation, and LC-MS-based metabolomics approach has a potential value for screening post-transplantation biomarkers.
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Journal: Converting Galactose into the Rare Sugar Talose with Cellobiose 2-Epimerase as Biocatalyst. (Pubmed Central) - Jul 19, 2019
...As the enzyme also catalyzes the keto-aldo isomerization of galactose to tagatose as a minor side reaction, the purity of talose was found to decrease over time...First, a mutant library of RmCE was created to try and increase the activity on monosaccharide substrates. Next, two residues from RmCE were introduced in the cellobiose 2-epimerase from Caldicellulosiruptor saccharolyticus (CsCE) (S99M/Q371F), increasing the k twofold.
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Journal: Microbial production of poly (glycolate-co-lactate-co-3-hydroxybutyrate) from glucose and xylose by Escherichia coli (Pubmed Central) - Jun 15, 2019
...The combinatorial biosynthetic route was constructed by the overexpression of a series of enzymes including D-tagatose 3-epimerase, L-fuculokinase, L-fuculose-phosphate aldolase, aldehyde dehydrogenase, propionyl-CoA transferase, β-ketothiolase, acetoacetyl-CoA reductase, and polyhydroxyalkanoate synthase...A co-culture engineering strategy was developed to produce biopolymer from a mixture of glucose and xylose, achieving 4.01 g/L cell dry weight containing 21.54% (W/W) biopolymer. The results of this work offer an approach for simultaneously utilizing glucose and xylose and indicate the potential for future biopolymer production from lignocellulosic biomass.
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Journal: One-Step Immobilization and Stabilization of a Recombinant Enterococcus faecium DBFIQ E36 L-Arabinose Isomerase for D-Tagatose Synthesis. (Pubmed Central) - May 29, 2019
The ionic interaction established between agarose gels containing monoaminoethyl-N-aminoethyl structures (MANAE) and the enzyme was the most suitable strategy for L-AI immobilization in comparison to the chelate-activated agarose. In addition, the immobilized biocatalysts by ionic interaction in MANAE showed to be the most stable, retaining up to 100% of enzyme activity for 60 min at 60 °C and with K values of 28 and 218 mM for MANAE-N-His-L-AI and MANAE-C-His-L-AI, respectively.
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Journal: Metabolic engineering pathways for rare sugars biosynthesis, physiological functionalities, and applications - A review. (Pubmed Central) - May 29, 2019
This article presents a comprehensive review of physiological functions and biotechnological applications of rare ketohexoses and aldohexoses, including D-psicose, D-tagatose, L-tagatose, D-sorbose, L-fructose, D-allose, L-glucose, D-gulose, L-talose, L-galactose, and L-fucose. Novel in-vivo recombination pathways based on aldolase and phosphatase for the biosynthesis of rare sugars, particularly D-psicose and D-sorbose using robust microbial strains are also deliberated.
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Journal: Effect of Respiratory Growth on the Metabolite Production and Stress Robustness of Lactobacillus casei N87 Cultivated in Cheese Whey Permeate Medium. (Pubmed Central) - May 10, 2019
...Genomic information suggested that membrane PTS system and tagatose-6-P pathway mediated the metabolism of lactose and galactose in L. casei N87...This study confirmed that the respiration-based technology coupled with cultivation on low-cost medium may be effectively exploited to produce competitive and functional starter and/or adjunct cultures. Our results, additionally, provided further information on the activation and regulation of metabolic pathways in homofermentative LAB grown under respiratory promoting conditions.
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Preclinical, Journal: Effect of the Lactobacillus rhamnosus strain GG and tagatose as synbiotic combination in a dextran sulfate sodium-induced colitis murine model. (Pubmed Central) - Apr 28, 2019
In particular, the abundance of Bacteroides, Lactobacillus, and Akkermansia was significantly associated with probiotic, prebiotic, and synbiotic treatments. Taken together, our results suggest that LGG and tagatose as synbiotics can alleviate colitis, and synbiotics could be applied as dietary supplements in dairy foods such as yogurt and cheese.
- |||||||||| Ac-225 rosopatamab tetraxetan (CONV01-?) / Dominari Holdings
Enrollment open, Metastases: 7R01CA207645-03: Phase I Trial of 225Ac-J591 in Patients With mCRPC (clinicaltrials.gov) - Oct 18, 2017
P1, N=42, Recruiting,
These results suggest that in vivo oxidoreaductase reactions can be employed to replace isomerases in vitro for biotransformation. Not yet recruiting --> Recruiting
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