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Biomarker, Journal: Serum biomarker analysis at the protein level on pulmonary hypertension secondary to old anterior myocardial infarction. (Pubmed Central) - Dec 8, 2020
Receiver operating characteristic curve analysis revealed haptoglobin (cut-off value: 78.295, sensitivity: 62.8%, specificity: 94.4%), annexin A5 (cut-off value: 151.925, sensitivity: 41.9%, specificity: 82.4%), and Ig mu chain C region (cut-off value: 168.885, sensitivity: 86.0%, specificity: 79.6%) (P<0.01). Three circulating serum proteins can be useful for the categorization of OAMI patients with and without PH.
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Journal: Proteomic Analysis of Porcine-Derived Collagen Membrane and Matrix. (Pubmed Central) - Nov 22, 2020
Examples of membrane-only proteins are COL12A1 and COL14A1, and, of matrix only proteins, elastin (ELN). The proteomic signature thus revealed the similarities between but also some individual proteins of collagen membrane and matrix.
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Biomarker, Journal: Proteomic identification of early urinary-biomarkers of acute kidney injury in preterm infants. (Pubmed Central) - Nov 14, 2020
Finally, enzyme-linked immunosorbent assays revealed that the levels of annexin A5, neutrophil gelatinase-associated lipocalin (NGAL), and protein S100-P were significantly higher in the samples of the first urine from patients with AKI than in those from patients without AKI. In conclusion, urinary annexin A5, NGAL and protein S100-P levels are promising biomarkers for early, accurate prediction of AKI in preterm infants.
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Journal: Analysis of Gene Expression of Pseudoarthrosis Tissue in Congenital Short Femur, Initial Study and First Exprerience. (Pubmed Central) - Nov 11, 2020
Some genes were considered over-expressed in PFFD tissue compared with the control sample (e.g. the gene for calcitonin receptor, collagen XII, I alpha 2, collagen II, IX, FGFR2, fibronectin, integrin) and other genes under-expressed (e.g. the gene for annexin A5, collagen XVIII alpha1, collagen I alpha1, cathepsin K, FGFR1, FGFR3, IGF2, VEGFB)...So far, the results cannot be generalized; this is the first step for follow-up experiments to confirm the suggested information and to integrate it with clinical findings, such as the alternative blood supply of affected extremity in some patients. Key words: proximal femoral focal deficiency, gene expression, microarray analysis, angiogenesis, osteogenesis.
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Journal: Chemical Proteomic Profiling of Lysophosphatidic Acid-binding Proteins. (Pubmed Central) - Oct 10, 2020
Together, we developed a novel LPA probe for the identification and characterizations of LPA-binding proteins from the entire human proteome. The method should be adaptable for the identification of other lipid-binding proteins.
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Journal, IO Biomarker: Calcium relieves fluoride-induced bone damage through the PI3K/AKT pathway. (Pubmed Central) - Oct 10, 2020
On the other hand, CaCO3 supplementation reversed the histopathological injury along with the levels of ALP, StrACP and serum Ca, alleviating the gene expression levels of PI3K/AKT pathway-related markers. Altogether, we can conclude that CaCO3 supplementation mitigated F-induced bone damage via the PI3K/AKT signaling pathway.
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Journal: Differential Effects of Human SP-A1 and SP-A2 on the BAL Proteome and Signaling Pathways in Response to Klebsiella pneumoniae and Ozone Exposure. (Pubmed Central) - Sep 26, 2020
(5) Eleven out of the 36 proteins, including annexin A5, glutathione S-transferase A4, SP-A1/SP-A2, and 14-3-3 zeta protein, exhibited significant differences among SP-A genotypes...These associated with SP-A genotype, sex, and ozone-induced oxidative stress in response to infection. We concluded that human SP-A2 and SP-A1 exhibit differential genotype-and sex-dependent innate immune responses to microbial pathogens and/or ozone-induced oxidative stress by modulating proteomic patterns and signaling pathways in the lung.
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Clinical, Journal: Predicting the risk of portal vein thrombosis in patients with liver cirrhosis and hepatocellular carcinoma. (Pubmed Central) - Sep 10, 2020
In our hypothesis, As phosphatidylserine exposure increase due to increased level of circulating microparticles in cirrhotics with and without HCC, anenxin-A5 may be secreted by platelets and endothelial cells into the circulation as a physiological response to inactivate the elevated levels of PS bearing MPs produced in these patients but the increase in anenxin-A5 level isn't equivalent to the increase in PS bearing MPs levels. The equilibrium between plasma annexin A5 and PS bearing MPs levels is defected.
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Journal: A scaffold for signaling of Tim-4-mediated efferocytosis is formed by fibronectin. (Pubmed Central) - Sep 2, 2020
By comparing efferocytosis induced by Tim-4 with that by Anxa5-GPI, an artificial tethering receptor, we found that Tim-4 possesses auxiliary machinery to induce a higher level of efferocytosis than Anxa5-GPI...In addition, Tim-4 depletion attenuated integrin signaling activation and perturbation of integrin signaling suppressed Tim-4-promoted efferocytosis. Taken together, the data suggest that Fn1 locates Tim-4 and integrins in close proximity by acting as a scaffold, resulting in synergistic cooperation of Tim-4 with integrins for efficient efferocytosis.
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Journal: Human amyloid-β enriched extracts: evaluation of in vitro and in vivo internalization and molecular characterization. (Pubmed Central) - Jul 18, 2020
Major improvement is needed before topical bevacizumab delivery can be considered clinically feasible. Identification and characterization of the unique features of these extracts, in terms of amyloid-β enrichment, identification of the components, in vitro and in vivo cell internalization, and tissue distribution, constitute the best initial tool to further investigate the seeding and transmissibility proposed in the prion-like hypothesis of Alzheimer's disease.
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Journal: Annexin A5 regulates surface αvβ5 integrin for retinal clearance phagocytosis. (Pubmed Central) - Jul 18, 2020
The effect of ANXA5 on surface αvβ5 and on particle binding requires the C-terminal ANXA5 annexin repeat but not its unique N-terminus. These results identify a novel role for ANXA5 specifically in the recognition/binding step of clearance phagocytosis that is essential to retinal physiology.
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Journal: GCA links TRAF6-ULK1-dependent autophagy activation in resistant chronic myeloid leukemia. (Pubmed Central) - Jul 16, 2020
Our findings represent the basis for novel therapeutic strategies against CML. Abbreviation: ACTB/β-actin: actin beta; ADM: adrenomedullin; AMBRA1: autophagy and beclin 1 regulator 1; AMPK: AMP-activated protein kinase; ANXA5: annexin A5; CP: cytogenetic response; CML: chronic myeloid leukemia; CUL3: cullin 3; GAPDH: glyceraldehyde-3-phosphate dehydrogenase; GCA: grancalcin; Dx: at diagnosis; E-64-d: (2S,3S)-trans-Epoxysuccinyl-L-leucylamido-3-methylbutane ethyl ester; IMres: Imatinib resistance; KLHL20: Kelch-like protein 20; LRMP: lymphoid-restricted membrane protein; MAP1LC3/LC3: microtubule associated protein 1 light chain 3; MMR: major molecular response; NHCl: ammonium chloride; PBMCs: peripheral blood mononuclear cells; PTPRC: protein tyrosine phosphatase, receptor type, C; SQSTM1/p62: sequestosome 1; SYK: spleen associated tyrosine kinase; TAP1: transporter 1, ATP binding cassette subfamily B member; TKIs: ABL-specific tyrosine kinase inhibitors; TLR9: toll- like receptor 9; TRAF6: TNF receptor associated factor 6; ULK1: unc-51 like autophagy activating kinase 1.
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Journal: Proteomic analysis of uterine fluid of fertile and subfertile hens before and after insemination. (Pubmed Central) - Jul 16, 2020
Immunohistochemistry was used to identify high (ANXA4/ANXA5/OCX32) and low (HSPA8/PIGR) fertility markers in the uterovaginal junction...In conclusion, sperm induced significant changes in the UF proteomic content. This study also provides evidence that the SST immune system plays a major role in regulating sperm storage.
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[VIRTUAL] Regulation of airways smooth muscle contraction through RG-1 in asthma: a proteomic approach (Pre-congress content) - Jul 15, 2020 - Abstract #ERS2020ERS_3634;
Of these, protein phosphatase 1 glycogen-associated regulatory subunit (RG1), protein FAM3A, desmin, actin-binding LIM protein-2, filamin-A, collagen type I alpha-1 chain, spastic paraplegia-7 protein, annexin-A5, tropomyosin, semaphorin-3F, testis-expressed protein-15, DNA-dependent protein kinase catalytic subunit, neuroepithelial cell-transforming gene-1 protein and calponin-1 were detected in both groups but showed an increase in asthma model...In conclusion, it is possible that during asthma the regulation ASM relaxation through MLCP might depend on RG1 instead MYPT1. SEP-CONACyT (# 256197)
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Journal, IO Biomarker: Mitophagy regulates mitochondrial network signaling, oxidative stress, and apoptosis during myoblast differentiation. (Pubmed Central) - Jul 7, 2020
Importantly, administration of a chemical inhibitor of CASP9 (Ac-LEHD-CHO) or dominant-negative CASP9 (ad-DNCASP9) partially recovered differentiation and myogenesis in shAtg7 myoblasts. Together, these data demonstrate an essential role for autophagy in protecting myoblasts from mitochondrial oxidative stress and apoptotic signaling during differentiation, as well as in the regulation of mitochondrial network remodelling and myogenesis.
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Journal: Whole-exome sequencing in multiplex preeclampsia families identifies novel candidate susceptibility genes. (Pubmed Central) - Jun 19, 2020
The study represents the first to use WES in multiplex families for preeclampsia and identifies two novel genes (QRFPR and C1orf35) not previously associated with preeclampsia and find nominal association of rs34270076 with protein levels, a key clinical feature of preeclampsia. We find further support for ANXA5 previously associated with pregnancy complications, including preeclampsia.
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Journal: Calcium Regulates the Nuclear Localization of Protein Arginine Deiminase 2. (Pubmed Central) - Jun 4, 2020
We hypothesize that this latter interaction promotes the translocation of PAD2 into the nucleus. These findings highlight a critical role for ANXA5 in regulating PAD2 and identify an unusual mechanism whereby proteins translocate between the cytosol and nucleus.
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Journal: Embryonic exposure to pentabromobenzene inhibited the inflation of posterior swim bladder in zebrafish larvae. (Pubmed Central) - May 30, 2020
In addition, the mRNA levels of anxa5, prlra, prlrb, atp1b2 and slc12a10 were all significantly changed at 120 hpf...The observed changes of thyroid and adrenocortical parameters might be indirect effects evoked by PBB exposure. Overall, our results provide important data and indications for future toxicological study and risk assessment of the emerging flame retardants PBB.
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Journal, Checkpoint inhibition: Annexin A5 as an immune checkpoint inhibitor and tumor-homing molecule for cancer treatment. (Pubmed Central) - May 27, 2020
Also, the therapeutic antitumor effect of an AnxA5-peptide fusion can be further enhanced by administration of other immune checkpoint inhibitors. Our findings support the administration of AnxA5 following chemotherapy as a promising immune checkpoint inhibitor for cancer treatment.
- |||||||||| chloroquine phosphate / Generic mfg., hydroxychloroquine / Generic mfg.
Review, Journal: The possible mechanisms of action of 4-aminoquinolines (chloroquine/hydroxychloroquine) against Sars-Cov-2 infection (COVID-19): A role for iron homeostasis? (Pubmed Central) - May 16, 2020
Here we review what is currently known on the mechanisms of action of CQ and HCQ as anti-viral, anti-inflammatory and anti-thrombotic drugs and discuss the up-to-date experimental evidence on the potential mechanisms of action of CQ/HCQ in Sars-Cov2 infection and the current clinical knowledge on their efficacy in the treatment of COVID-19 patients. Given the role of iron in several human viral infections, we also propose a different insight into a number of CQ and HCQ pharmacological effects, suggesting a potential involvement of iron homeostasis in Sars-Cov-2 infection and COVID-19 clinical course.
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Journal: Association between ALS and retroviruses: evidence from bioinformatics analysis. (Pubmed Central) - Mar 26, 2020
The neuroinflammation and apoptotic regulation pathways are specifically implicated. The continuation and further analysis of large scale genome studies may prove useful in exploring genes important in retroviral activation and ALS, which may help discover new drug targets.
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Journal: The role of ANXA5 in DBP-induced oxidative stress through ERK/Nrf2 pathway. (Pubmed Central) - Mar 18, 2020
Ultimately, we demonstrated ANXA5 could increase ERK phosphorylated level and the activated role of ANXA5 on ERK/Nrf2 pathway could be reversed by ERK inhibitor. Overall, this study illuminated that ANXA5 could defend testicle Leydig and Sertoli cells against DBP-induced oxidative stress injury through ERK/Nrf2 pathway.
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Single Cell Transcriptomic Profiling of Alveolar Epithelial Type 2 Cells in Sftpa1 G231V Mice (PENNSYLVANIA CONVENTION CENTER, Room 122 A-B (100 Level)) - Mar 15, 2020 - Abstract #ATS2020ATS_4148;
Single cell RNA sequencing demonstrated enrichment of ER stress genes in AT2 cells isolated from mutant CRISPR-Cas9-generated mouse model of familial pulmonary fibrosis as compared to wild type. Subtypes of AT2 cells were distinguished as having roles in wound healing and cell division.
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Objective Assessment of DARC in Human Eyes: Manual Observers versus Automated AI Algorithm (Exhibit Hall: Posterboard# A0350) - Mar 9, 2020 - Abstract #ARVO2020ARVO_749;
Purpose DARC (Detection of Apoptosing Retinal Cells) is a novel technique enabling the visualization of sick and apoptosing retinal cells using intravenous Anx776 - a fluorescently labelled annexin A5...In comparison, the CNN algorithm had an AUC of 0.89 with sensitivity of 0.86 and specificity of 0.92 to glaucoma progression.Conclusions Comparison of manual observer counts shows poor agreement between the 5 observers, and this was highlighted by the ROC parameters. In comparison, the CNN-aided algorithm appeared better, and has promise as an automated and objective biomarker.Acknowledgements: Melanie Almonte, Paolo Bonetti, Jessica Bonetti, Benjamin Davis, Serge Miodragovic, Tim Yap, Eduardo Normando, Philip Bloom, Saad Younis, Madelein Walpert, Richard Nicholas, and all our Phase 2 subjects.
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